Restriction map for pF1KB8985
 (Linear) (Six-base) MAPSORT of: KIBB8985.seq  Check: 1849  from: 1  to: 1161



KIBB8985.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:08  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     893    1161 
   Size:        893     268
 
AatII G_ACGT'C

Cuts at:      0     373    1161 
   Size:        373     788
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     332     924    1161 
   Size:        332     592     237

  Fragments arranged by size: 

                 592     332     237
 
AflIII A'CryG_T

Cuts at:      0     824    1161 
   Size:        824     337
 
ApaLI G'TGCA_C

Cuts at:      0    1100    1161 
   Size:       1100      61
 
AvaI C'yCGr_G

Cuts at:      0      76     499     720    1161 
   Size:         76     423     221     441

  Fragments arranged by size: 

                 441     423     221      76
 
BanI G'GyrC_C

Cuts at:      0     290     336     881    1119    1161 
   Size:        290      46     545     238      42

  Fragments arranged by size: 

                 545     290     238      46      42
 
BanII G_rGCy'C

Cuts at:      0     470     506     613    1161 
   Size:        470      36     107     548

  Fragments arranged by size: 

                 548     470     107      36
 
BbeI G_GCGC'C

Cuts at:      0    1123    1161 
   Size:       1123      38
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     431    1161 
   Size:        431     730
 
BglI GCCn_nnn'nGGC

Cuts at:      0     541     768    1161 
   Size:        541     227     393

  Fragments arranged by size: 

                 541     393     227
 
BlpI GC'TnA_GC

Cuts at:      0     358    1161 
   Size:        358     803
 
Bme1580I G_kGCm'C

Cuts at:      0     341     832    1104    1161 
   Size:        341     491     272      57

  Fragments arranged by size: 

                 491     341     272      57
 
BmgBI CAC'GTC

Cuts at:      0     408    1161 
   Size:        408     753
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     703    1161 
   Size:        703     458
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     742    1051    1161 
   Size:        742     309     110

  Fragments arranged by size: 

                 742     309     110
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     679    1161 
   Size:        679     482
 
BsaHI Gr'CG_yC

Cuts at:      0     370    1120    1161 
   Size:        370     750      41

  Fragments arranged by size: 

                 750     370      41
 
BsaWI w'CCGG_w

Cuts at:      0     618    1161 
   Size:        618     543
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      91     121     199     229     403     433     595     625 
   Size:         91      30      78      30     174      30     162      30

Cuts at:    625    1161 
   Size:        536

  Fragments arranged by size: 

                 536     174     162      91      78      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      94    1161 
   Size:         94    1067
 
BseYI C'CCAG_C

Cuts at:      0      88     740    1161 
   Size:         88     652     421

  Fragments arranged by size: 

                 652     421      88
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     409    1161 
   Size:        409     752
 
BsiEI CG_ry'CG

Cuts at:      0     836     849    1161 
   Size:        836      13     312

  Fragments arranged by size: 

                 836     312      13
 
BsiHKAI G_wGCw'C

Cuts at:      0     626    1104    1161 
   Size:        626     478      57

  Fragments arranged by size: 

                 626     478      57
 
BsmI GAATG_Cn'

Cuts at:      0     697    1161 
   Size:        697     464
 
Bsp1286I G_dGCh'C

Cuts at:      0     341     470     506     613     626     832    1104    1161 
   Size:        341     129      36     107      13     206     272      57

  Fragments arranged by size: 

                 341     272     206     129     107      57      36      13
            
 
BspEI T'CCGG_A

Cuts at:      0     618    1161 
   Size:        618     543
 
BspHI T'CATG_A

Cuts at:      0     314    1161 
   Size:        314     847
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     893    1161 
   Size:        893     268
 
BsrBI CCG'CTC

Cuts at:      0     750     807    1161 
   Size:        750      57     354

  Fragments arranged by size: 

                 750     354      57
 
BsrFI r'CCGG_y

Cuts at:      0       4      27     322     540     788    1161 
   Size:          4      23     295     218     248     373

  Fragments arranged by size: 

                 373     295     248     218      23       4
 
BssHII G'CGCG_C

Cuts at:      0      92    1027    1161 
   Size:         92     935     134

  Fragments arranged by size: 

                 935     134      92
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1108    1161 
   Size:       1108      53
 
BstEII G'GTnAC_C

Cuts at:      0      30    1146    1161 
   Size:         30    1116      15

  Fragments arranged by size: 

                1116      30      15
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     606    1161 
   Size:        606     555
 
BstYI r'GATC_y

Cuts at:      0      83     713     923    1058    1161 
   Size:         83     630     210     135     103

  Fragments arranged by size: 

                 630     210     135     103      83
 
BtgI C'CryG_G

Cuts at:      0     374    1142    1161 
   Size:        374     768      19

  Fragments arranged by size: 

                 768     374      19
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     127    1161 
   Size:        127    1034
 
EaeI y'GGCC_r

Cuts at:      0     377     533     833    1161 
   Size:        377     156     300     328

  Fragments arranged by size: 

                 377     328     300     156
 
EagI C'GGCC_G

Cuts at:      0     833    1161 
   Size:        833     328
 
EarI CTCTTCn'nnn_

Cuts at:      0     109     565    1161 
   Size:        109     456     596

  Fragments arranged by size: 

                 596     456     109
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1102    1161 
   Size:       1102      59
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     332     703     924    1161 
   Size:        332     371     221     237

  Fragments arranged by size: 

                 371     332     237     221
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     285    1161 
   Size:        285     876
 
FspI TGC'GCA

Cuts at:      0     889    1161 
   Size:        889     272
 
HaeII r_GCGC'y

Cuts at:      0     323     358    1050    1123    1161 
   Size:        323      35     692      73      38

  Fragments arranged by size: 

                 692     323      73      38      35
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     445     457     477     489     514     546     595     627 
   Size:        445      12      20      12      25      32      49      32

Cuts at:    627    1161 
   Size:        534

  Fragments arranged by size: 

                 534     445      49      32      32      25      20      12
                  12
 
HincII GTy'rAC

Cuts at:      0     660    1161 
   Size:        660     501
 
KasI G'GCGC_C

Cuts at:      0    1119    1161 
   Size:       1119      42
 
MluI A'CGCG_T

Cuts at:      0     824    1161 
   Size:        824     337
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     308     467     527    1161 
   Size:        308     159      60     634

  Fragments arranged by size: 

                 634     308     159      60
 
NaeI GCC'GGC

Cuts at:      0     324     542    1161 
   Size:        324     218     619

  Fragments arranged by size: 

                 619     324     218
 
NarI GG'CG_CC

Cuts at:      0    1120    1161 
   Size:       1120      41
 
NgoMIV G'CCGG_C

Cuts at:      0     322     540    1161 
   Size:        322     218     621

  Fragments arranged by size: 

                 621     322     218
 
NspI r_CATG'y

Cuts at:      0     933    1161 
   Size:        933     228
 
PasI CC'CwG_GG

Cuts at:      0     489    1161 
   Size:        489     672
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     642    1161 
   Size:        642     519
 
PshAI GACnn'nnGTC

Cuts at:      0     170    1161 
   Size:        170     991
 
SfcI C'TryA_G

Cuts at:      0     149    1161 
   Size:        149    1012
 
SfoI GGC'GCC

Cuts at:      0    1121    1161 
   Size:       1121      40
 
SmaI CCC'GGG

Cuts at:      0     501     722    1161 
   Size:        501     221     439

  Fragments arranged by size: 

                 501     439     221
 
SmlI C'TyrA_G

Cuts at:      0     757    1066    1161 
   Size:        757     309      95

  Fragments arranged by size: 

                 757     309      95
 
SphI G_CATG'C

Cuts at:      0     933    1161 
   Size:        933     228
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     160    1161 
   Size:        160    1001
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     381     543     724    1161 
   Size:        381     162     181     437

  Fragments arranged by size: 

                 437     381     181     162
 
XmaI C'CCGG_G

Cuts at:      0     499     720    1161 
   Size:        499     221     441

  Fragments arranged by size: 

                 499     441     221
 
ZraI GAC'GTC

Cuts at:      0     371    1161 
   Size:        371     790
 

 Enzymes that do cut:

     AarI    AatII     AcuI   AflIII    ApaLI     AvaI     BanI    BanII
     BbeI    BciVI     BglI     BlpI Bme1580I    BmgBI     BpmI    BpuEI
     BsaI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrFI
   BssHII   BstAPI   BstEII    BstXI    BstYI     BtgI     DrdI     EaeI
     EagI     EarI     EciI  Eco57MI     FalI     FspI    HaeII    Hin4I
   HincII     KasI     MluI     MmeI     NaeI     NarI   NgoMIV     NspI
     PasI    PflMI    PshAI     SfcI     SfoI     SmaI     SmlI     SphI
    TaqII     XcmI     XmaI     ZraI

 Enzymes that do not cut: 

     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI     AleI
     AlfI     AloI    AlwNI     ApaI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BbsI    BbvCI     BcgI     BclI    BfrBI
    BglII     BmrI     BmtI     BplI   Bpu10I    BsaAI    BsaBI    BsiWI
    BsmBI    BsrDI    BsrGI    BssSI    BstBI  BstZ17I   Bsu36I    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII  EcoICRI    EcoNI EcoO109I
    EcoRI    EcoRV     FseI    FspAI  HindIII     HpaI     KpnI     MfeI
     MscI     MslI   MspA1I     NcoI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SgfI    SgrAI    SnaBI     SpeI     SrfI     SspI     StuI
     StyI     SwaI     TatI     TstI  Tth111I     XbaI     XhoI     XmnI
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