(Linear) (Six-base) MAPSORT of: KIBB8985.seq Check: 1849 from: 1 to: 1161 KIBB8985.orf With 182 enzymes: SgfI * April 24, 2009 13:08 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 893 1161 Size: 893 268 AatII G_ACGT'C Cuts at: 0 373 1161 Size: 373 788 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 332 924 1161 Size: 332 592 237 Fragments arranged by size: 592 332 237 AflIII A'CryG_T Cuts at: 0 824 1161 Size: 824 337 ApaLI G'TGCA_C Cuts at: 0 1100 1161 Size: 1100 61 AvaI C'yCGr_G Cuts at: 0 76 499 720 1161 Size: 76 423 221 441 Fragments arranged by size: 441 423 221 76 BanI G'GyrC_C Cuts at: 0 290 336 881 1119 1161 Size: 290 46 545 238 42 Fragments arranged by size: 545 290 238 46 42 BanII G_rGCy'C Cuts at: 0 470 506 613 1161 Size: 470 36 107 548 Fragments arranged by size: 548 470 107 36 BbeI G_GCGC'C Cuts at: 0 1123 1161 Size: 1123 38 BciVI GTATCCnnnnn_n' Cuts at: 0 431 1161 Size: 431 730 BglI GCCn_nnn'nGGC Cuts at: 0 541 768 1161 Size: 541 227 393 Fragments arranged by size: 541 393 227 BlpI GC'TnA_GC Cuts at: 0 358 1161 Size: 358 803 Bme1580I G_kGCm'C Cuts at: 0 341 832 1104 1161 Size: 341 491 272 57 Fragments arranged by size: 491 341 272 57 BmgBI CAC'GTC Cuts at: 0 408 1161 Size: 408 753 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 703 1161 Size: 703 458 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 742 1051 1161 Size: 742 309 110 Fragments arranged by size: 742 309 110 BsaI GGTCTCn'nnnn_ Cuts at: 0 679 1161 Size: 679 482 BsaHI Gr'CG_yC Cuts at: 0 370 1120 1161 Size: 370 750 41 Fragments arranged by size: 750 370 41 BsaWI w'CCGG_w Cuts at: 0 618 1161 Size: 618 543 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 91 121 199 229 403 433 595 625 Size: 91 30 78 30 174 30 162 30 Cuts at: 625 1161 Size: 536 Fragments arranged by size: 536 174 162 91 78 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 94 1161 Size: 94 1067 BseYI C'CCAG_C Cuts at: 0 88 740 1161 Size: 88 652 421 Fragments arranged by size: 652 421 88 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 409 1161 Size: 409 752 BsiEI CG_ry'CG Cuts at: 0 836 849 1161 Size: 836 13 312 Fragments arranged by size: 836 312 13 BsiHKAI G_wGCw'C Cuts at: 0 626 1104 1161 Size: 626 478 57 Fragments arranged by size: 626 478 57 BsmI GAATG_Cn' Cuts at: 0 697 1161 Size: 697 464 Bsp1286I G_dGCh'C Cuts at: 0 341 470 506 613 626 832 1104 1161 Size: 341 129 36 107 13 206 272 57 Fragments arranged by size: 341 272 206 129 107 57 36 13 BspEI T'CCGG_A Cuts at: 0 618 1161 Size: 618 543 BspHI T'CATG_A Cuts at: 0 314 1161 Size: 314 847 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 893 1161 Size: 893 268 BsrBI CCG'CTC Cuts at: 0 750 807 1161 Size: 750 57 354 Fragments arranged by size: 750 354 57 BsrFI r'CCGG_y Cuts at: 0 4 27 322 540 788 1161 Size: 4 23 295 218 248 373 Fragments arranged by size: 373 295 248 218 23 4 BssHII G'CGCG_C Cuts at: 0 92 1027 1161 Size: 92 935 134 Fragments arranged by size: 935 134 92 BstAPI GCAn_nnn'nTGC Cuts at: 0 1108 1161 Size: 1108 53 BstEII G'GTnAC_C Cuts at: 0 30 1146 1161 Size: 30 1116 15 Fragments arranged by size: 1116 30 15 BstXI CCAn_nnnn'nTGG Cuts at: 0 606 1161 Size: 606 555 BstYI r'GATC_y Cuts at: 0 83 713 923 1058 1161 Size: 83 630 210 135 103 Fragments arranged by size: 630 210 135 103 83 BtgI C'CryG_G Cuts at: 0 374 1142 1161 Size: 374 768 19 Fragments arranged by size: 768 374 19 DrdI GACnn_nn'nnGTC Cuts at: 0 127 1161 Size: 127 1034 EaeI y'GGCC_r Cuts at: 0 377 533 833 1161 Size: 377 156 300 328 Fragments arranged by size: 377 328 300 156 EagI C'GGCC_G Cuts at: 0 833 1161 Size: 833 328 EarI CTCTTCn'nnn_ Cuts at: 0 109 565 1161 Size: 109 456 596 Fragments arranged by size: 596 456 109 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1102 1161 Size: 1102 59 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 332 703 924 1161 Size: 332 371 221 237 Fragments arranged by size: 371 332 237 221 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 285 1161 Size: 285 876 FspI TGC'GCA Cuts at: 0 889 1161 Size: 889 272 HaeII r_GCGC'y Cuts at: 0 323 358 1050 1123 1161 Size: 323 35 692 73 38 Fragments arranged by size: 692 323 73 38 35 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 445 457 477 489 514 546 595 627 Size: 445 12 20 12 25 32 49 32 Cuts at: 627 1161 Size: 534 Fragments arranged by size: 534 445 49 32 32 25 20 12 12 HincII GTy'rAC Cuts at: 0 660 1161 Size: 660 501 KasI G'GCGC_C Cuts at: 0 1119 1161 Size: 1119 42 MluI A'CGCG_T Cuts at: 0 824 1161 Size: 824 337 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 308 467 527 1161 Size: 308 159 60 634 Fragments arranged by size: 634 308 159 60 NaeI GCC'GGC Cuts at: 0 324 542 1161 Size: 324 218 619 Fragments arranged by size: 619 324 218 NarI GG'CG_CC Cuts at: 0 1120 1161 Size: 1120 41 NgoMIV G'CCGG_C Cuts at: 0 322 540 1161 Size: 322 218 621 Fragments arranged by size: 621 322 218 NspI r_CATG'y Cuts at: 0 933 1161 Size: 933 228 PasI CC'CwG_GG Cuts at: 0 489 1161 Size: 489 672 PflMI CCAn_nnn'nTGG Cuts at: 0 642 1161 Size: 642 519 PshAI GACnn'nnGTC Cuts at: 0 170 1161 Size: 170 991 SfcI C'TryA_G Cuts at: 0 149 1161 Size: 149 1012 SfoI GGC'GCC Cuts at: 0 1121 1161 Size: 1121 40 SmaI CCC'GGG Cuts at: 0 501 722 1161 Size: 501 221 439 Fragments arranged by size: 501 439 221 SmlI C'TyrA_G Cuts at: 0 757 1066 1161 Size: 757 309 95 Fragments arranged by size: 757 309 95 SphI G_CATG'C Cuts at: 0 933 1161 Size: 933 228 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 160 1161 Size: 160 1001 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 381 543 724 1161 Size: 381 162 181 437 Fragments arranged by size: 437 381 181 162 XmaI C'CCGG_G Cuts at: 0 499 720 1161 Size: 499 221 441 Fragments arranged by size: 499 441 221 ZraI GAC'GTC Cuts at: 0 371 1161 Size: 371 790 Enzymes that do cut: AarI AatII AcuI AflIII ApaLI AvaI BanI BanII BbeI BciVI BglI BlpI Bme1580I BmgBI BpmI BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI BssHII BstAPI BstEII BstXI BstYI BtgI DrdI EaeI EagI EarI EciI Eco57MI FalI FspI HaeII Hin4I HincII KasI MluI MmeI NaeI NarI NgoMIV NspI PasI PflMI PshAI SfcI SfoI SmaI SmlI SphI TaqII XcmI XmaI ZraI Enzymes that do not cut: AccI Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI BclI BfrBI BglII BmrI BmtI BplI Bpu10I BsaAI BsaBI BsiWI BsmBI BsrDI BsrGI BssSI BstBI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII EcoICRI EcoNI EcoO109I EcoRI EcoRV FseI FspAI HindIII HpaI KpnI MfeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NsiI PacI PciI PfoI PmeI PmlI PpiI PpuMI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SrfI SspI StuI StyI SwaI TatI TstI Tth111I XbaI XhoI XmnI