Restriction map for
pF1KB8982
(Linear) (Six-base) MAPSORT of: KIBB8982.seq Check: 6784 from: 1 to: 1134
KIBB8982.orf
With 182 enzymes: SgfI *
March 3, 2008 11:09 ..
AleI CACnn'nnGTG
Cuts at: 0 995 1134
Size: 995 139
ApaI G_GGCC'C
Cuts at: 0 203 1134
Size: 203 931
ApoI r'AATT_y
Cuts at: 0 630 1134
Size: 630 504
AvaI C'yCGr_G
Cuts at: 0 76 570 1134
Size: 76 494 564
Fragments arranged by size:
564 494 76
BanI G'GyrC_C
Cuts at: 0 21 414 549 775 820 1123 1134
Size: 21 393 135 226 45 303 11
Fragments arranged by size:
393 303 226 135 45 21 11
BanII G_rGCy'C
Cuts at: 0 7 74 203 880 916 923 1134
Size: 7 67 129 677 36 7 211
Fragments arranged by size:
677 211 129 67 36 7 7
BbeI G_GCGC'C
Cuts at: 0 25 824 1134
Size: 25 799 310
Fragments arranged by size:
799 310 25
BbsI GAAGACnn'nnnn_
Cuts at: 0 703 1134
Size: 703 431
BbvCI CC'TCA_GC
Cuts at: 0 131 982 1134
Size: 131 851 152
Fragments arranged by size:
851 152 131
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1002 1036 1134
Size: 1002 34 98
Fragments arranged by size:
1002 98 34
BglI GCCn_nnn'nGGC
Cuts at: 0 685 1134
Size: 685 449
BglII A'GATC_T
Cuts at: 0 443 1134
Size: 443 691
BlpI GC'TnA_GC
Cuts at: 0 1036 1057 1134
Size: 1036 21 77
Fragments arranged by size:
1036 77 21
Bme1580I G_kGCm'C
Cuts at: 0 125 203 417 554 778 1126 1134
Size: 125 78 214 137 224 348 8
Fragments arranged by size:
348 224 214 137 125 78 8
BmrI ACTGGGnnnn_n'
Cuts at: 0 42 917 1017 1134
Size: 42 875 100 117
Fragments arranged by size:
875 117 100 42
Bpu10I CC'TnA_GC
Cuts at: 0 131 917 982 1134
Size: 131 786 65 152
Fragments arranged by size:
786 152 131 65
BsaAI yAC'GTr
Cuts at: 0 456 1134
Size: 456 678
BsaHI Gr'CG_yC
Cuts at: 0 22 821 1134
Size: 22 799 313
Fragments arranged by size:
799 313 22
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 949 979 1037 1067 1134
Size: 949 30 58 30 67
Fragments arranged by size:
949 67 58 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 361 1134
Size: 361 773
BseYI C'CCAG_C
Cuts at: 0 55 124 289 1134
Size: 55 69 165 845
Fragments arranged by size:
845 165 69 55
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 292 1134
Size: 292 842
BsiHKAI G_wGCw'C
Cuts at: 0 7 1058 1134
Size: 7 1051 76
Fragments arranged by size:
1051 76 7
Bsp1286I G_dGCh'C
Cuts at: 0 7 74 125 203 417 554 778 880
Size: 7 67 51 78 214 137 224 102
Cuts at: 880 916 923 1058 1126 1134
Size: 36 7 135 68 8
Fragments arranged by size:
224 214 137 135 102 78 68 67
51 36 8 7 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 23 233 1134
Size: 23 210 901
Fragments arranged by size:
901 210 23
BsrBI CCG'CTC
Cuts at: 0 869 972 1134
Size: 869 103 162
Fragments arranged by size:
869 162 103
BsrFI r'CCGG_y
Cuts at: 0 18 493 1134
Size: 18 475 641
Fragments arranged by size:
641 475 18
BssSI C'ACGA_G
Cuts at: 0 882 1134
Size: 882 252
BstXI CCAn_nnnn'nTGG
Cuts at: 0 265 997 1134
Size: 265 732 137
Fragments arranged by size:
732 265 137
BstYI r'GATC_y
Cuts at: 0 443 1048 1134
Size: 443 605 86
Fragments arranged by size:
605 443 86
Bsu36I CC'TnA_GG
Cuts at: 0 743 1134
Size: 743 391
BtgI C'CryG_G
Cuts at: 0 398 408 734 1134
Size: 398 10 326 400
Fragments arranged by size:
400 398 326 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 378 1134
Size: 378 756
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1080 1115 1134
Size: 1080 35 19
Fragments arranged by size:
1080 35 19
EaeI y'GGCC_r
Cuts at: 0 63 367 401 458 1134
Size: 63 304 34 57 676
Fragments arranged by size:
676 304 63 57 34
EarI CTCTTCn'nnn_
Cuts at: 0 643 1134
Size: 643 491
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 502 1134
Size: 502 632
EcoICRI GAG'CTC
Cuts at: 0 5 1134
Size: 5 1129
EcoNI CCTnn'n_nnAGG
Cuts at: 0 256 1134
Size: 256 878
HaeII r_GCGC'y
Cuts at: 0 25 429 824 1134
Size: 25 404 395 310
Fragments arranged by size:
404 395 310 25
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 652 684 748 780 1040 1072 1134
Size: 652 32 64 32 260 32 62
Fragments arranged by size:
652 260 64 62 32 32 32
HincII GTy'rAC
Cuts at: 0 1092 1134
Size: 1092 42
KasI G'GCGC_C
Cuts at: 0 21 820 1134
Size: 21 799 314
Fragments arranged by size:
799 314 21
MscI TGG'CCA
Cuts at: 0 403 1134
Size: 403 731
MslI CAynn'nnrTG
Cuts at: 0 397 995 1030 1134
Size: 397 598 35 104
Fragments arranged by size:
598 397 104 35
MspA1I CmG'CkG
Cuts at: 0 135 282 995 1134
Size: 135 147 713 139
Fragments arranged by size:
713 147 139 135
NaeI GCC'GGC
Cuts at: 0 20 495 1134
Size: 20 475 639
Fragments arranged by size:
639 475 20
NarI GG'CG_CC
Cuts at: 0 22 821 1134
Size: 22 799 313
Fragments arranged by size:
799 313 22
NcoI C'CATG_G
Cuts at: 0 398 1134
Size: 398 736
NgoMIV G'CCGG_C
Cuts at: 0 18 493 1134
Size: 18 475 641
Fragments arranged by size:
641 475 18
NspI r_CATG'y
Cuts at: 0 1035 1134
Size: 1035 99
PasI CC'CwG_GG
Cuts at: 0 1115 1134
Size: 1115 19
PflMI CCAn_nnn'nTGG
Cuts at: 0 456 894 1134
Size: 456 438 240
Fragments arranged by size:
456 438 240
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 601 633 1134
Size: 601 32 501
Fragments arranged by size:
601 501 32
PspOMI G'GGCC_C
Cuts at: 0 199 1134
Size: 199 935
PstI C_TGCA'G
Cuts at: 0 43 1134
Size: 43 1091
SacI G_AGCT'C
Cuts at: 0 7 1134
Size: 7 1127
SfcI C'TryA_G
Cuts at: 0 39 1134
Size: 39 1095
SfoI GGC'GCC
Cuts at: 0 23 822 1134
Size: 23 799 312
Fragments arranged by size:
799 312 23
SmaI CCC'GGG
Cuts at: 0 572 1134
Size: 572 562
StuI AGG'CCT
Cuts at: 0 262 1134
Size: 262 872
StyI C'CwwG_G
Cuts at: 0 398 683 893 1134
Size: 398 285 210 241
Fragments arranged by size:
398 285 241 210
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 125 1134
Size: 125 1009
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1037 1069 1134
Size: 1037 32 65
Fragments arranged by size:
1037 65 32
XmaI C'CCGG_G
Cuts at: 0 570 1134
Size: 570 564
XmnI GAAnn'nnTTC
Cuts at: 0 103 616 1134
Size: 103 513 518
Fragments arranged by size:
518 513 103
Enzymes that do cut:
AleI ApaI ApoI AvaI BanI BanII BbeI BbsI
BbvCI BcgI BglI BglII BlpI Bme1580I BmrI Bpu10I
BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I
BspMI BsrBI BsrFI BssSI BstXI BstYI Bsu36I BtgI
BtgZI CspCI EaeI EarI EciI EcoICRI EcoNI HaeII
Hin4I HincII KasI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PflMI PpiI PspOMI PstI
SacI SfcI SfoI SmaI StuI StyI TaqII TstI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AlfI AloI AlwNI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BciVI BclI BfrBI
BmgBI BmtI BplI BpmI BpuEI BsaI BsaBI BsaWI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrGI
BssHII BstAPI BstBI BstEII BstZ17I BtsI ClaI DraI
DraIII DrdI EagI Eco57MI EcoO109I EcoRI EcoRV FalI
FseI FspI FspAI HindIII HpaI KpnI MfeI MluI
MmeI NdeI NheI NotI NruI NsiI PacI PciI
PfoI PmeI PmlI PpuMI PshAI PsiI PspXI PsrI
PvuI PvuII RsrII SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SgfI SgrAI SmlI SnaBI SpeI
SphI SrfI SspI SwaI TatI Tth111I XbaI XcmI
XhoI ZraI