Restriction map for
pF1KB8980
(Linear) (Six-base) MAPSORT of: KIBB8980.seq Check: 5030 from: 1 to: 1128
KIBB8980.orf
With 182 enzymes: SgfI *
March 3, 2008 11:09 ..
AfeI AGC'GCT
Cuts at: 0 533 1128
Size: 533 595
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 268 1128
Size: 268 860
AlwNI CAG_nnn'CTG
Cuts at: 0 1119 1128
Size: 1119 9
ApaI G_GGCC'C
Cuts at: 0 726 1128
Size: 726 402
ApoI r'AATT_y
Cuts at: 0 10 481 1128
Size: 10 471 647
Fragments arranged by size:
647 471 10
AvaI C'yCGr_G
Cuts at: 0 277 1128
Size: 277 851
BanI G'GyrC_C
Cuts at: 0 252 262 431 1128
Size: 252 10 169 697
Fragments arranged by size:
697 252 169 10
BanII G_rGCy'C
Cuts at: 0 229 726 1128
Size: 229 497 402
Fragments arranged by size:
497 402 229
BbeI G_GCGC'C
Cuts at: 0 266 435 1128
Size: 266 169 693
Fragments arranged by size:
693 266 169
BbsI GAAGACnn'nnnn_
Cuts at: 0 694 1128
Size: 694 434
BbvCI CC'TCA_GC
Cuts at: 0 379 1128
Size: 379 749
BglI GCCn_nnn'nGGC
Cuts at: 0 233 1128
Size: 233 895
Bme1580I G_kGCm'C
Cuts at: 0 257 726 1128
Size: 257 469 402
Fragments arranged by size:
469 402 257
BmrI ACTGGGnnnn_n'
Cuts at: 0 600 811 1019 1128
Size: 600 211 208 109
Fragments arranged by size:
600 211 208 109
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 975 1128
Size: 975 153
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 751 1128
Size: 751 377
Bpu10I CC'TnA_GC
Cuts at: 0 379 709 1128
Size: 379 330 419
Fragments arranged by size:
419 379 330
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 670 970 1128
Size: 670 300 158
Fragments arranged by size:
670 300 158
BsaHI Gr'CG_yC
Cuts at: 0 263 432 1128
Size: 263 169 696
Fragments arranged by size:
696 263 169
BsaWI w'CCGG_w
Cuts at: 0 578 1128
Size: 578 550
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 137 685 1128
Size: 137 548 443
Fragments arranged by size:
548 443 137
BseYI C'CCAG_C
Cuts at: 0 169 1128
Size: 169 959
BsiEI CG_ry'CG
Cuts at: 0 213 335 1128
Size: 213 122 793
Fragments arranged by size:
793 213 122
BsmBI CGTCTCn'nnnn_
Cuts at: 0 720 1128
Size: 720 408
Bsp1286I G_dGCh'C
Cuts at: 0 229 257 726 1128
Size: 229 28 469 402
Fragments arranged by size:
469 402 229 28
BsrDI GCAATG_nn'
Cuts at: 0 843 1128
Size: 843 285
BsrFI r'CCGG_y
Cuts at: 0 259 330 364 1128
Size: 259 71 34 764
Fragments arranged by size:
764 259 71 34
BstXI CCAn_nnnn'nTGG
Cuts at: 0 205 913 1128
Size: 205 708 215
Fragments arranged by size:
708 215 205
Bsu36I CC'TnA_GG
Cuts at: 0 941 1128
Size: 941 187
BtgI C'CryG_G
Cuts at: 0 198 230 240 1128
Size: 198 32 10 888
Fragments arranged by size:
888 198 32 10
EaeI y'GGCC_r
Cuts at: 0 210 308 332 1128
Size: 210 98 24 796
Fragments arranged by size:
796 210 98 24
EagI C'GGCC_G
Cuts at: 0 210 332 1128
Size: 210 122 796
Fragments arranged by size:
796 210 122
EarI CTCTTCn'nnn_
Cuts at: 0 677 1128
Size: 677 451
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 751 1128
Size: 751 377
EcoO109I rG'GnC_Cy
Cuts at: 0 722 723 945 954 1128
Size: 722 1 222 9 174
Fragments arranged by size:
722 222 174 9 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 711 1128
Size: 711 417
HaeII r_GCGC'y
Cuts at: 0 206 266 435 535 1128
Size: 206 60 169 100 593
Fragments arranged by size:
593 206 169 100 60
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 132 164 1128
Size: 132 32 964
Fragments arranged by size:
964 132 32
HincII GTy'rAC
Cuts at: 0 896 1128
Size: 896 232
KasI G'GCGC_C
Cuts at: 0 262 431 1128
Size: 262 169 697
Fragments arranged by size:
697 262 169
MspA1I CmG'CkG
Cuts at: 0 232 242 416 1066 1128
Size: 232 10 174 650 62
Fragments arranged by size:
650 232 174 62 10
NaeI GCC'GGC
Cuts at: 0 261 332 1128
Size: 261 71 796
Fragments arranged by size:
796 261 71
NarI GG'CG_CC
Cuts at: 0 263 432 1128
Size: 263 169 696
Fragments arranged by size:
696 263 169
NgoMIV G'CCGG_C
Cuts at: 0 259 330 1128
Size: 259 71 798
Fragments arranged by size:
798 259 71
NotI GC'GGCC_GC
Cuts at: 0 210 1128
Size: 210 918
PpuMI rG'GwC_Cy
Cuts at: 0 954 1128
Size: 954 174
PspOMI G'GGCC_C
Cuts at: 0 722 1128
Size: 722 406
PstI C_TGCA'G
Cuts at: 0 272 1004 1128
Size: 272 732 124
Fragments arranged by size:
732 272 124
PvuII CAG'CTG
Cuts at: 0 1066 1128
Size: 1066 62
SacII CC_GC'GG
Cuts at: 0 233 243 1128
Size: 233 10 885
Fragments arranged by size:
885 233 10
SfcI C'TryA_G
Cuts at: 0 268 1000 1128
Size: 268 732 128
Fragments arranged by size:
732 268 128
SfoI GGC'GCC
Cuts at: 0 264 433 1128
Size: 264 169 695
Fragments arranged by size:
695 264 169
SgrAI Cr'CCGG_yG
Cuts at: 0 259 1128
Size: 259 869
SmlI C'TyrA_G
Cuts at: 0 649 949 1128
Size: 649 300 179
Fragments arranged by size:
649 300 179
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 888 1038 1128
Size: 888 150 90
Fragments arranged by size:
888 150 90
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 376 408 1128
Size: 376 32 720
Fragments arranged by size:
720 376 32
XbaI T'CTAG_A
Cuts at: 0 465 1128
Size: 465 663
Enzymes that do cut:
AfeI AlfI AlwNI ApaI ApoI AvaI BanI BanII
BbeI BbsI BbvCI BglI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaHI BsaWI BseRI BseYI BsiEI BsmBI
Bsp1286I BsrDI BsrFI BstXI Bsu36I BtgI EaeI EagI
EarI Eco57MI EcoO109I FalI HaeII Hin4I HincII KasI
MspA1I NaeI NarI NgoMIV NotI PpuMI PspOMI PstI
PvuII SacII SfcI SfoI SgrAI SmlI TaqII TstI
XbaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AloI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BcgI BciVI BclI BfrBI BglII
BlpI BmgBI BmtI BsaI BsaAI BsaBI BsaXI BsgI
BsiHKAI BsiWI BsmI BspEI BspHI BspMI BsrBI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EciI EcoICRI
EcoNI EcoRI EcoRV FseI FspI FspAI HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI NcoI NdeI
NheI NruI NsiI NspI PacI PasI PciI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PvuI RsrII SacI SalI SanDI SapI SbfI ScaI
SexAI SfiI SgfI SmaI SnaBI SpeI SphI SrfI
SspI StuI StyI SwaI TatI Tth111I XcmI XhoI
XmaI XmnI ZraI