Restriction map for
pF1KB8978
(Linear) (Six-base) MAPSORT of: KIBB8978.seq Check: 60 from: 1 to: 1122
KIBB8978.orf
With 182 enzymes: SgfI *
March 3, 2008 11:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 852 1122
Size: 852 270
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 238 1087 1112 1122
Size: 238 849 25 10
Fragments arranged by size:
849 238 25 10
AleI CACnn'nnGTG
Cuts at: 0 862 1122
Size: 862 260
ApaI G_GGCC'C
Cuts at: 0 126 1122
Size: 126 996
ApoI r'AATT_y
Cuts at: 0 100 808 1122
Size: 100 708 314
Fragments arranged by size:
708 314 100
AvaI C'yCGr_G
Cuts at: 0 666 1122
Size: 666 456
BanII G_rGCy'C
Cuts at: 0 126 297 1089 1122
Size: 126 171 792 33
Fragments arranged by size:
792 171 126 33
BbsI GAAGACnn'nnnn_
Cuts at: 0 1093 1122
Size: 1093 29
BclI T'GATC_A
Cuts at: 0 485 1122
Size: 485 637
Bme1580I G_kGCm'C
Cuts at: 0 126 1122
Size: 126 996
BmgBI CAC'GTC
Cuts at: 0 108 1122
Size: 108 1014
BsaI GGTCTCn'nnnn_
Cuts at: 0 1005 1122
Size: 1005 117
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1031 1061 1122
Size: 1031 30 61
Fragments arranged by size:
1031 61 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1039 1122
Size: 1039 83
BsiHKAI G_wGCw'C
Cuts at: 0 90 1122
Size: 90 1032
BsmI GAATG_Cn'
Cuts at: 0 333 1122
Size: 333 789
Bsp1286I G_dGCh'C
Cuts at: 0 90 126 297 1089 1122
Size: 90 36 171 792 33
Fragments arranged by size:
792 171 90 36 33
BspHI T'CATG_A
Cuts at: 0 743 1122
Size: 743 379
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 248 852 1122
Size: 248 604 270
Fragments arranged by size:
604 270 248
BsrBI CCG'CTC
Cuts at: 0 47 141 1122
Size: 47 94 981
Fragments arranged by size:
981 94 47
BsrDI GCAATG_nn'
Cuts at: 0 338 1122
Size: 338 784
BssSI C'ACGA_G
Cuts at: 0 584 1122
Size: 584 538
BstEII G'GTnAC_C
Cuts at: 0 459 1122
Size: 459 663
BstXI CCAn_nnnn'nTGG
Cuts at: 0 590 846 1122
Size: 590 256 276
Fragments arranged by size:
590 276 256
BtsI GCAGTG_nn'
Cuts at: 0 728 888 1122
Size: 728 160 234
Fragments arranged by size:
728 234 160
ClaI AT'CG_AT
Cuts at: 0 407 1122
Size: 407 715
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 51 86 1122
Size: 51 35 1036
Fragments arranged by size:
1036 51 35
DraI TTT'AAA
Cuts at: 0 446 1122
Size: 446 676
EaeI y'GGCC_r
Cuts at: 0 251 1122
Size: 251 871
EarI CTCTTCn'nnn_
Cuts at: 0 703 766 1122
Size: 703 63 356
Fragments arranged by size:
703 356 63
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 392 1122
Size: 392 730
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 238 1087 1112 1122
Size: 238 849 25 10
Fragments arranged by size:
849 238 25 10
EcoO109I rG'GnC_Cy
Cuts at: 0 122 1122
Size: 122 1000
EcoRI G'AATT_C
Cuts at: 0 808 1122
Size: 808 314
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1117 1122
Size: 1117 5
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 858 890 1122
Size: 858 32 232
Fragments arranged by size:
858 232 32
HincII GTy'rAC
Cuts at: 0 844 1122
Size: 844 278
MfeI C'AATT_G
Cuts at: 0 1076 1122
Size: 1076 46
MscI TGG'CCA
Cuts at: 0 253 1122
Size: 253 869
MslI CAynn'nnrTG
Cuts at: 0 588 610 742 862 1122
Size: 588 22 132 120 260
Fragments arranged by size:
588 260 132 120 22
MspA1I CmG'CkG
Cuts at: 0 642 1122
Size: 642 480
NspI r_CATG'y
Cuts at: 0 183 1122
Size: 183 939
PflMI CCAn_nnn'nTGG
Cuts at: 0 994 1122
Size: 994 128
PfoI T'CCnGG_A
Cuts at: 0 803 1002 1122
Size: 803 199 120
Fragments arranged by size:
803 199 120
PspOMI G'GGCC_C
Cuts at: 0 122 1122
Size: 122 1000
SexAI A'CCwGG_T
Cuts at: 0 490 1122
Size: 490 632
SfcI C'TryA_G
Cuts at: 0 963 1122
Size: 963 159
SspI AAT'ATT
Cuts at: 0 780 1122
Size: 780 342
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1010 1031 1042 1063 1122
Size: 1010 21 11 21 59
Fragments arranged by size:
1010 59 21 21 11
XmnI GAAnn'nnTTC
Cuts at: 0 598 799 1122
Size: 598 201 323
Fragments arranged by size:
598 323 201
Enzymes that do cut:
AarI AcuI AleI ApaI ApoI AvaI BanII BbsI
BclI Bme1580I BmgBI BsaI BsaXI BseRI BsiHKAI BsmI
Bsp1286I BspHI BspMI BsrBI BsrDI BssSI BstEII BstXI
BtsI ClaI CspCI DraI EaeI EarI EciI Eco57MI
EcoO109I EcoRI FalI Hin4I HincII MfeI MscI MslI
MspA1I NspI PflMI PfoI PspOMI SexAI SfcI SspI
TstI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI AlwNI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BanI BbeI BbvCI BcgI BciVI
BfrBI BglI BglII BlpI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BseYI BsgI
BsiEI BsiWI BsmBI BspEI BsrFI BsrGI BssHII BstAPI
BstBI BstYI BstZ17I Bsu36I BtgI BtgZI DraIII DrdI
EagI EcoICRI EcoNI EcoRV FseI FspI FspAI HaeII
HindIII HpaI KasI KpnI MluI MmeI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PmeI PmlI PpiI PpuMI PshAI PsiI
PspXI PsrI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI StuI
StyI SwaI TaqII TatI Tth111I XbaI XcmI XhoI
XmaI ZraI