Restriction map for
pF1KB8976
(Linear) (Six-base) MAPSORT of: KIBB8976.seq Check: 1058 from: 1 to: 1116
KIBB8976.orf
With 182 enzymes: SgfI *
March 3, 2008 11:09 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 880 1116
Size: 880 236
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 427 1116
Size: 427 689
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 34 66 1116
Size: 34 32 1050
Fragments arranged by size:
1050 34 32
ApaI G_GGCC'C
Cuts at: 0 131 1116
Size: 131 985
ApaLI G'TGCA_C
Cuts at: 0 724 1116
Size: 724 392
AvaI C'yCGr_G
Cuts at: 0 31 1116
Size: 31 1085
BanI G'GyrC_C
Cuts at: 0 233 749 1116
Size: 233 516 367
Fragments arranged by size:
516 367 233
BanII G_rGCy'C
Cuts at: 0 131 334 1116
Size: 131 203 782
Fragments arranged by size:
782 203 131
BbeI G_GCGC'C
Cuts at: 0 753 1116
Size: 753 363
BbsI GAAGACnn'nnnn_
Cuts at: 0 294 1116
Size: 294 822
BbvCI CC'TCA_GC
Cuts at: 0 755 1116
Size: 755 361
Bme1580I G_kGCm'C
Cuts at: 0 131 164 728 1116
Size: 131 33 564 388
Fragments arranged by size:
564 388 131 33
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 247 1116
Size: 61 186 869
Fragments arranged by size:
869 186 61
Bpu10I CC'TnA_GC
Cuts at: 0 755 1116
Size: 755 361
BsaI GGTCTCn'nnnn_
Cuts at: 0 253 1116
Size: 253 863
BsaAI yAC'GTr
Cuts at: 0 714 1116
Size: 714 402
BsaHI Gr'CG_yC
Cuts at: 0 750 1116
Size: 750 366
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 34 64 73 103 574 604 1116
Size: 34 30 9 30 471 30 512
Fragments arranged by size:
512 471 34 30 30 30 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 478 1116
Size: 478 638
BseYI C'CCAG_C
Cuts at: 0 341 743 1017 1116
Size: 341 402 274 99
Fragments arranged by size:
402 341 274 99
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 367 1116
Size: 367 749
BsiHKAI G_wGCw'C
Cuts at: 0 728 1116
Size: 728 388
BsmI GAATG_Cn'
Cuts at: 0 1098 1116
Size: 1098 18
Bsp1286I G_dGCh'C
Cuts at: 0 131 164 334 728 1116
Size: 131 33 170 394 388
Fragments arranged by size:
394 388 170 131 33
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 886 1116
Size: 886 230
BsrBI CCG'CTC
Cuts at: 0 231 1116
Size: 231 885
BsrFI r'CCGG_y
Cuts at: 0 236 973 1027 1116
Size: 236 737 54 89
Fragments arranged by size:
737 236 89 54
BstAPI GCAn_nnn'nTGC
Cuts at: 0 732 1116
Size: 732 384
BstEII G'GTnAC_C
Cuts at: 0 815 1116
Size: 815 301
BstXI CCAn_nnnn'nTGG
Cuts at: 0 522 1116
Size: 522 594
BtgI C'CryG_G
Cuts at: 0 164 254 281 320 431 866 902 1116
Size: 164 90 27 39 111 435 36 214
Fragments arranged by size:
435 214 164 111 90 39 36 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 411 1116
Size: 411 705
BtsI GCAGTG_nn'
Cuts at: 0 939 996 1116
Size: 939 57 120
Fragments arranged by size:
939 120 57
DraIII CAC_nnn'GTG
Cuts at: 0 917 1116
Size: 917 199
EaeI y'GGCC_r
Cuts at: 0 278 284 323 446 779 905 1116
Size: 278 6 39 123 333 126 211
Fragments arranged by size:
333 278 211 126 123 39 6
EarI CTCTTCn'nnn_
Cuts at: 0 562 1057 1116
Size: 562 495 59
Fragments arranged by size:
562 495 59
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 247 880 1116
Size: 61 186 633 236
Fragments arranged by size:
633 236 186 61
EcoO109I rG'GnC_Cy
Cuts at: 0 45 127 128 345 353 1116
Size: 45 82 1 217 8 763
Fragments arranged by size:
763 217 82 45 8 1
HaeII r_GCGC'y
Cuts at: 0 753 1116
Size: 753 363
HincII GTy'rAC
Cuts at: 0 393 1116
Size: 393 723
KasI G'GCGC_C
Cuts at: 0 749 1116
Size: 749 367
MscI TGG'CCA
Cuts at: 0 448 781 907 1116
Size: 448 333 126 209
Fragments arranged by size:
448 333 209 126
MslI CAynn'nnrTG
Cuts at: 0 430 694 787 913 1116
Size: 430 264 93 126 203
Fragments arranged by size:
430 264 203 126 93
MspA1I CmG'CkG
Cuts at: 0 747 759 1116
Size: 747 12 357
Fragments arranged by size:
747 357 12
NaeI GCC'GGC
Cuts at: 0 975 1116
Size: 975 141
NarI GG'CG_CC
Cuts at: 0 750 1116
Size: 750 366
NgoMIV G'CCGG_C
Cuts at: 0 973 1116
Size: 973 143
NspI r_CATG'y
Cuts at: 0 640 1116
Size: 640 476
PfoI T'CCnGG_A
Cuts at: 0 224 839 1116
Size: 224 615 277
Fragments arranged by size:
615 277 224
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 34 66 1116
Size: 34 32 1050
Fragments arranged by size:
1050 34 32
PpuMI rG'GwC_Cy
Cuts at: 0 45 345 353 1116
Size: 45 300 8 763
Fragments arranged by size:
763 300 45 8
PspOMI G'GGCC_C
Cuts at: 0 127 1116
Size: 127 989
PstI C_TGCA'G
Cuts at: 0 406 1116
Size: 406 710
SapI GCTCTTCn'nnn_
Cuts at: 0 1057 1116
Size: 1057 59
SfcI C'TryA_G
Cuts at: 0 171 402 1116
Size: 171 231 714
Fragments arranged by size:
714 231 171
SfoI GGC'GCC
Cuts at: 0 751 1116
Size: 751 365
SgrAI Cr'CCGG_yG
Cuts at: 0 973 1116
Size: 973 143
SmlI C'TyrA_G
Cuts at: 0 31 1116
Size: 31 1085
SphI G_CATG'C
Cuts at: 0 640 1116
Size: 640 476
StyI C'CwwG_G
Cuts at: 0 573 1116
Size: 573 543
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 574 606 1116
Size: 574 32 510
Fragments arranged by size:
574 510 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 505 522 696 1116
Size: 505 17 174 420
Fragments arranged by size:
505 420 174 17
XhoI C'TCGA_G
Cuts at: 0 31 1116
Size: 31 1085
Enzymes that do cut:
AcuI AlfI AloI ApaI ApaLI AvaI BanI BanII
BbeI BbsI BbvCI Bme1580I BpmI Bpu10I BsaI BsaAI
BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspMI BsrBI BsrFI BstAPI BstEII BstXI BtgI BtgZI
BtsI DraIII EaeI EarI Eco57MI EcoO109I HaeII HincII
KasI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PfoI PpiI PpuMI PspOMI PstI SapI SfcI SfoI
SgrAI SmlI SphI StyI TstI XcmI XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlwNI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BcgI BciVI BclI BfrBI BglI
BglII BlpI BmgBI BmrI BmtI BplI BpuEI BsaBI
BsaWI BsiEI BsiWI BsmBI BspEI BspHI BsrDI BsrGI
BssHII BssSI BstBI BstYI BstZ17I Bsu36I ClaI CspCI
DraI DrdI EagI EciI EcoICRI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI Hin4I HindIII HpaI KpnI
MfeI MluI MmeI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PciI PflMI PmeI PmlI PshAI
PsiI PspXI PsrI PvuI PvuII RsrII SacI SacII
SalI SanDI SbfI ScaI SexAI SfiI SgfI SmaI
SnaBI SpeI SrfI SspI StuI SwaI TaqII TatI
Tth111I XbaI XmaI XmnI ZraI