Restriction map for
pF1KB8974
(Linear) (Six-base) MAPSORT of: KIBB8974.seq Check: 4993 from: 1 to: 1107
KIBB8974.orf
With 182 enzymes: SgfI *
March 3, 2008 11:09 ..
AccI GT'mk_AC
Cuts at: 0 215 1107
Size: 215 892
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 649 670 1015 1107
Size: 649 21 345 92
Fragments arranged by size:
649 345 92 21
AflIII A'CryG_T
Cuts at: 0 251 1107
Size: 251 856
AleI CACnn'nnGTG
Cuts at: 0 232 1107
Size: 232 875
ApoI r'AATT_y
Cuts at: 0 264 1107
Size: 264 843
BanI G'GyrC_C
Cuts at: 0 184 1107
Size: 184 923
BbsI GAAGACnn'nnnn_
Cuts at: 0 83 244 731 1107
Size: 83 161 487 376
Fragments arranged by size:
487 376 161 83
BclI T'GATC_A
Cuts at: 0 407 1107
Size: 407 700
BmrI ACTGGGnnnn_n'
Cuts at: 0 1017 1107
Size: 1017 90
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1104 1107
Size: 1104 3
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1099 1107
Size: 1099 8
BsaHI Gr'CG_yC
Cuts at: 0 839 1107
Size: 839 268
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 274 1107
Size: 274 833
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 210 576 847 1107
Size: 210 366 271 260
Fragments arranged by size:
366 271 260 210
BsiHKAI G_wGCw'C
Cuts at: 0 462 622 1098 1107
Size: 462 160 476 9
Fragments arranged by size:
476 462 160 9
Bsp1286I G_dGCh'C
Cuts at: 0 462 622 1098 1107
Size: 462 160 476 9
Fragments arranged by size:
476 462 160 9
BsrDI GCAATG_nn'
Cuts at: 0 314 434 440 581 1107
Size: 314 120 6 141 526
Fragments arranged by size:
526 314 141 120 6
BsrGI T'GTAC_A
Cuts at: 0 374 488 1107
Size: 374 114 619
Fragments arranged by size:
619 374 114
BssSI C'ACGA_G
Cuts at: 0 169 982 1107
Size: 169 813 125
Fragments arranged by size:
813 169 125
BstEII G'GTnAC_C
Cuts at: 0 900 1107
Size: 900 207
BstYI r'GATC_y
Cuts at: 0 978 1107
Size: 978 129
Bsu36I CC'TnA_GG
Cuts at: 0 1089 1107
Size: 1089 18
BtgI C'CryG_G
Cuts at: 0 71 596 1107
Size: 71 525 511
Fragments arranged by size:
525 511 71
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 626 897 1107
Size: 626 271 210
Fragments arranged by size:
626 271 210
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 864 899 1107
Size: 864 35 208
Fragments arranged by size:
864 208 35
EarI CTCTTCn'nnn_
Cuts at: 0 305 1107
Size: 305 802
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 961 1107
Size: 961 146
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 649 670 1015 1099 1107
Size: 649 21 345 84 8
Fragments arranged by size:
649 345 84 21 8
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 402 1107
Size: 402 705
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 37 69 1107
Size: 37 32 1038
Fragments arranged by size:
1038 37 32
HincII GTy'rAC
Cuts at: 0 87 366 1107
Size: 87 279 741
Fragments arranged by size:
741 279 87
HindIII A'AGCT_T
Cuts at: 0 1053 1107
Size: 1053 54
HpaI GTT'AAC
Cuts at: 0 87 1107
Size: 87 1020
MfeI C'AATT_G
Cuts at: 0 272 1107
Size: 272 835
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 884 1107
Size: 884 223
MslI CAynn'nnrTG
Cuts at: 0 232 421 1107
Size: 232 189 686
Fragments arranged by size:
686 232 189
MspA1I CmG'CkG
Cuts at: 0 395 529 637 1107
Size: 395 134 108 470
Fragments arranged by size:
470 395 134 108
NcoI C'CATG_G
Cuts at: 0 71 596 1107
Size: 71 525 511
Fragments arranged by size:
525 511 71
NspI r_CATG'y
Cuts at: 0 255 375 1107
Size: 255 120 732
Fragments arranged by size:
732 255 120
PciI A'CATG_T
Cuts at: 0 251 1107
Size: 251 856
PvuII CAG'CTG
Cuts at: 0 395 529 637 1107
Size: 395 134 108 470
Fragments arranged by size:
470 395 134 108
SfcI C'TryA_G
Cuts at: 0 390 1107
Size: 390 717
StyI C'CwwG_G
Cuts at: 0 71 188 596 1107
Size: 71 117 408 511
Fragments arranged by size:
511 408 117 71
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 21 172 262 919 949 1107
Size: 21 151 90 657 30 158
Fragments arranged by size:
657 158 151 90 30 21
TatI w'GTAC_w
Cuts at: 0 161 374 488 1107
Size: 161 213 114 619
Fragments arranged by size:
619 213 161 114
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 69 161 519 1107
Size: 69 92 358 588
Fragments arranged by size:
588 358 92 69
Enzymes that do cut:
AccI AcuI AflIII AleI ApoI BanI BbsI BclI
BmrI BplI BpmI BsaHI BseRI BsgI BsiHKAI Bsp1286I
BsrDI BsrGI BssSI BstEII BstYI Bsu36I BtgI BtgZI
CspCI EarI EciI Eco57MI FalI Hin4I HincII HindIII
HpaI MfeI MmeI MslI MspA1I NcoI NspI PciI
PvuII SfcI StyI TaqII TatI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanII BbeI BbvCI BcgI
BciVI BfrBI BglI BglII BlpI Bme1580I BmgBI BmtI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BseYI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI
BsrFI BssHII BstAPI BstBI BstXI BstZ17I BtsI ClaI
DraI DraIII DrdI EaeI EagI EcoICRI EcoNI EcoO109I
EcoRI EcoRV FseI FspI FspAI HaeII KasI KpnI
MluI MscI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI SwaI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI