Restriction map for
pF1KB8970
(Linear) (Six-base) MAPSORT of: KIBB8970.seq Check: 7185 from: 1 to: 1077
KIBB8970.orf
With 182 enzymes: SgfI *
March 3, 2008 11:09 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 63 920 1077
Size: 63 857 157
Fragments arranged by size:
857 157 63
AleI CACnn'nnGTG
Cuts at: 0 37 384 1077
Size: 37 347 693
Fragments arranged by size:
693 347 37
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 402 1077
Size: 402 675
AlwNI CAG_nnn'CTG
Cuts at: 0 725 771 1077
Size: 725 46 306
Fragments arranged by size:
725 306 46
AvaI C'yCGr_G
Cuts at: 0 129 1077
Size: 129 948
AvrII C'CTAG_G
Cuts at: 0 399 1077
Size: 399 678
BanI G'GyrC_C
Cuts at: 0 404 538 658 701 1077
Size: 404 134 120 43 376
Fragments arranged by size:
404 376 134 120 43
BanII G_rGCy'C
Cuts at: 0 350 577 1077
Size: 350 227 500
Fragments arranged by size:
500 350 227
BbeI G_GCGC'C
Cuts at: 0 662 1077
Size: 662 415
BbsI GAAGACnn'nnnn_
Cuts at: 0 171 917 1077
Size: 171 746 160
Fragments arranged by size:
746 171 160
BbvCI CC'TCA_GC
Cuts at: 0 764 1077
Size: 764 313
BglI GCCn_nnn'nGGC
Cuts at: 0 95 1077
Size: 95 982
BglII A'GATC_T
Cuts at: 0 979 1077
Size: 979 98
Bme1580I G_kGCm'C
Cuts at: 0 407 1077
Size: 407 670
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 591 1077
Size: 591 486
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 403 594 793 968 1077
Size: 403 191 199 175 109
Fragments arranged by size:
403 199 191 175 109
Bpu10I CC'TnA_GC
Cuts at: 0 235 764 1077
Size: 235 529 313
Fragments arranged by size:
529 313 235
BsaBI GATnn'nnATC
Cuts at: 0 933 967 1077
Size: 933 34 110
Fragments arranged by size:
933 110 34
BsaHI Gr'CG_yC
Cuts at: 0 153 659 1077
Size: 153 506 418
Fragments arranged by size:
506 418 153
BsaWI w'CCGG_w
Cuts at: 0 269 1077
Size: 269 808
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 350 380 694 724 1077
Size: 350 30 314 30 353
Fragments arranged by size:
353 350 314 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 342 583 790 1077
Size: 342 241 207 287
Fragments arranged by size:
342 287 241 207
BseYI C'CCAG_C
Cuts at: 0 135 219 588 1077
Size: 135 84 369 489
Fragments arranged by size:
489 369 135 84
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 544 699 1065 1077
Size: 544 155 366 12
Fragments arranged by size:
544 366 155 12
BsiHKAI G_wGCw'C
Cuts at: 0 77 148 605 818 1077
Size: 77 71 457 213 259
Fragments arranged by size:
457 259 213 77 71
Bsp1286I G_dGCh'C
Cuts at: 0 77 148 350 407 577 605 818 1077
Size: 77 71 202 57 170 28 213 259
Fragments arranged by size:
259 213 202 170 77 71 57 28
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 110 1077
Size: 110 967
BsrFI r'CCGG_y
Cuts at: 0 85 686 1040 1077
Size: 85 601 354 37
Fragments arranged by size:
601 354 85 37
BssHII G'CGCG_C
Cuts at: 0 124 491 738 1077
Size: 124 367 247 339
Fragments arranged by size:
367 339 247 124
BssSI C'ACGA_G
Cuts at: 0 565 1077
Size: 565 512
BstAPI GCAn_nnn'nTGC
Cuts at: 0 545 1077
Size: 545 532
BstEII G'GTnAC_C
Cuts at: 0 207 1077
Size: 207 870
BstXI CCAn_nnnn'nTGG
Cuts at: 0 39 381 618 1062 1077
Size: 39 342 237 444 15
Fragments arranged by size:
444 342 237 39 15
BstYI r'GATC_y
Cuts at: 0 979 1077
Size: 979 98
BtgI C'CryG_G
Cuts at: 0 374 1077
Size: 374 703
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 694 1077
Size: 694 383
BtsI GCAGTG_nn'
Cuts at: 0 157 358 1077
Size: 157 201 719
Fragments arranged by size:
719 201 157
DraIII CAC_nnn'GTG
Cuts at: 0 545 827 1077
Size: 545 282 250
Fragments arranged by size:
545 282 250
EaeI y'GGCC_r
Cuts at: 0 371 1077
Size: 371 706
EarI CTCTTCn'nnn_
Cuts at: 0 82 823 1077
Size: 82 741 254
Fragments arranged by size:
741 254 82
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 63 403 594 793 920 968 1077
Size: 63 340 191 199 127 48 109
Fragments arranged by size:
340 199 191 127 109 63 48
EcoNI CCTnn'n_nnAGG
Cuts at: 0 717 1077
Size: 717 360
EcoO109I rG'GnC_Cy
Cuts at: 0 893 1077
Size: 893 184
FspI TGC'GCA
Cuts at: 0 667 1077
Size: 667 410
HaeII r_GCGC'y
Cuts at: 0 473 625 662 805 1077
Size: 473 152 37 143 272
Fragments arranged by size:
473 272 152 143 37
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 413 445 1077
Size: 413 32 632
Fragments arranged by size:
632 413 32
KasI G'GCGC_C
Cuts at: 0 658 1077
Size: 658 419
MfeI C'AATT_G
Cuts at: 0 947 1077
Size: 947 130
MscI TGG'CCA
Cuts at: 0 373 1077
Size: 373 704
MslI CAynn'nnrTG
Cuts at: 0 37 190 384 1060 1077
Size: 37 153 194 676 17
Fragments arranged by size:
676 194 153 37 17
MspA1I CmG'CkG
Cuts at: 0 20 168 699 1077
Size: 20 148 531 378
Fragments arranged by size:
531 378 148 20
NarI GG'CG_CC
Cuts at: 0 659 1077
Size: 659 418
NcoI C'CATG_G
Cuts at: 0 374 1077
Size: 374 703
NspI r_CATG'y
Cuts at: 0 189 1077
Size: 189 888
PasI CC'CwG_GG
Cuts at: 0 111 1077
Size: 111 966
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 19 51 1077
Size: 19 32 1026
Fragments arranged by size:
1026 32 19
PpuMI rG'GwC_Cy
Cuts at: 0 893 1077
Size: 893 184
PstI C_TGCA'G
Cuts at: 0 698 1077
Size: 698 379
SapI GCTCTTCn'nnn_
Cuts at: 0 82 823 1077
Size: 82 741 254
Fragments arranged by size:
741 254 82
ScaI AGT'ACT
Cuts at: 0 529 1077
Size: 529 548
SexAI A'CCwGG_T
Cuts at: 0 541 1077
Size: 541 536
SfcI C'TryA_G
Cuts at: 0 694 1077
Size: 694 383
SfoI GGC'GCC
Cuts at: 0 660 1077
Size: 660 417
SgrAI Cr'CCGG_yG
Cuts at: 0 686 1077
Size: 686 391
StyI C'CwwG_G
Cuts at: 0 374 399 958 1077
Size: 374 25 559 119
Fragments arranged by size:
559 374 119 25
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 165 1077
Size: 165 912
TatI w'GTAC_w
Cuts at: 0 527 1077
Size: 527 550
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 350 382 694 726 1077
Size: 350 32 312 32 351
Fragments arranged by size:
351 350 312 32 32
Enzymes that do cut:
AcuI AleI AlfI AlwNI AvaI AvrII BanI BanII
BbeI BbsI BbvCI BglI BglII Bme1580I BplI BpmI
Bpu10I BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspMI BsrFI BssHII BssSI BstAPI BstEII
BstXI BstYI BtgI BtgZI BtsI DraIII EaeI EarI
Eco57MI EcoNI EcoO109I FspI HaeII Hin4I KasI MfeI
MscI MslI MspA1I NarI NcoI NspI PasI PpiI
PpuMI PstI SapI ScaI SexAI SfcI SfoI SgrAI
StyI TaqII TatI TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AloI ApaI ApaLI ApoI AscI AseI
AsiSI BaeI BamHI BcgI BciVI BclI BfrBI BlpI
BmgBI BmrI BmtI BpuEI BsaI BsaAI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrGI BstBI
BstZ17I Bsu36I ClaI CspCI DraI DrdI EagI EciI
EcoICRI EcoRI EcoRV FalI FseI FspAI HincII HindIII
HpaI KpnI MluI MmeI NaeI NdeI NgoMIV NheI
NotI NruI NsiI PacI PciI PflMI PfoI PmeI
PmlI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII
RsrII SacI SacII SalI SanDI SbfI SfiI SgfI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI