Restriction map for
pF1KB8965
(Linear) (Six-base) MAPSORT of: KIBB8965.seq Check: 5046 from: 1 to: 1062
KIBB8965.orf
With 182 enzymes: SgfI *
March 3, 2008 11:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 578 1062
Size: 578 484
AccI GT'mk_AC
Cuts at: 0 710 1062
Size: 710 352
AhdI GACnn_n'nnGTC
Cuts at: 0 9 1062
Size: 9 1053
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 562 1062
Size: 562 500
AlwNI CAG_nnn'CTG
Cuts at: 0 156 792 1062
Size: 156 636 270
Fragments arranged by size:
636 270 156
AvaI C'yCGr_G
Cuts at: 0 714 1062
Size: 714 348
BanI G'GyrC_C
Cuts at: 0 56 561 651 679 766 1062
Size: 56 505 90 28 87 296
Fragments arranged by size:
505 296 90 87 56 28
BanII G_rGCy'C
Cuts at: 0 187 331 407 448 721 1012 1062
Size: 187 144 76 41 273 291 50
Fragments arranged by size:
291 273 187 144 76 50 41
BbeI G_GCGC'C
Cuts at: 0 60 683 770 1062
Size: 60 623 87 292
Fragments arranged by size:
623 292 87 60
BbsI GAAGACnn'nnnn_
Cuts at: 0 43 951 1062
Size: 43 908 111
Fragments arranged by size:
908 111 43
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 347 381 1062
Size: 347 34 681
Fragments arranged by size:
681 347 34
BglII A'GATC_T
Cuts at: 0 143 1062
Size: 143 919
BlpI GC'TnA_GC
Cuts at: 0 136 1062
Size: 136 926
Bme1580I G_kGCm'C
Cuts at: 0 469 566 656 1062
Size: 469 97 90 406
Fragments arranged by size:
469 406 97 90
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 345 1062
Size: 345 717
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 508 871 1062
Size: 508 363 191
Fragments arranged by size:
508 363 191
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 361 1062
Size: 361 701
BsaHI Gr'CG_yC
Cuts at: 0 57 680 745 767 979 1062
Size: 57 623 65 22 212 83
Fragments arranged by size:
623 212 83 65 57 22
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 844 874 1062
Size: 844 30 188
Fragments arranged by size:
844 188 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 337 1062
Size: 337 725
BseYI C'CCAG_C
Cuts at: 0 550 888 931 1062
Size: 550 338 43 131
Fragments arranged by size:
550 338 131 43
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 850 1062
Size: 850 212
BsiEI CG_ry'CG
Cuts at: 0 346 452 623 1001 1062
Size: 346 106 171 378 61
Fragments arranged by size:
378 346 171 106 61
Bsp1286I G_dGCh'C
Cuts at: 0 187 331 407 448 469 566 656 721
Size: 187 144 76 41 21 97 90 65
Cuts at: 721 1012 1062
Size: 291 50
Fragments arranged by size:
291 187 144 97 90 76 65 50
41 21
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 578 1017 1062
Size: 578 439 45
Fragments arranged by size:
578 439 45
BsrFI r'CCGG_y
Cuts at: 0 46 451 622 676 682 1000 1062
Size: 46 405 171 54 6 318 62
Fragments arranged by size:
405 318 171 62 54 46 6
BsrGI T'GTAC_A
Cuts at: 0 386 1062
Size: 386 676
BssSI C'ACGA_G
Cuts at: 0 462 1062
Size: 462 600
BstEII G'GTnAC_C
Cuts at: 0 1029 1062
Size: 1029 33
BstXI CCAn_nnnn'nTGG
Cuts at: 0 480 1062
Size: 480 582
BstYI r'GATC_y
Cuts at: 0 143 1062
Size: 143 919
BtgI C'CryG_G
Cuts at: 0 749 809 910 974 1062
Size: 749 60 101 64 88
Fragments arranged by size:
749 101 88 64 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 69 1062
Size: 69 993
DraIII CAC_nnn'GTG
Cuts at: 0 561 1062
Size: 561 501
EaeI y'GGCC_r
Cuts at: 0 101 302 343 449 513 585 620 733
Size: 101 201 41 106 64 72 35 113
Cuts at: 733 812 998 1062
Size: 79 186 64
Fragments arranged by size:
201 186 113 106 101 79 72 64
64 41 35
EagI C'GGCC_G
Cuts at: 0 343 449 620 998 1062
Size: 343 106 171 378 64
Fragments arranged by size:
378 343 171 106 64
EarI CTCTTCn'nnn_
Cuts at: 0 990 1062
Size: 990 72
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 508 871 1062
Size: 508 363 191
Fragments arranged by size:
508 363 191
EcoO109I rG'GnC_Cy
Cuts at: 0 258 1062
Size: 258 804
HaeII r_GCGC'y
Cuts at: 0 60 129 376 487 683 757 770 1062
Size: 60 69 247 111 196 74 13 292
Fragments arranged by size:
292 247 196 111 74 69 60 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 27 1062
Size: 27 1035
KasI G'GCGC_C
Cuts at: 0 56 679 766 1062
Size: 56 623 87 296
Fragments arranged by size:
623 296 87 56
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 602 1062
Size: 602 460
MscI TGG'CCA
Cuts at: 0 103 1062
Size: 103 959
MslI CAynn'nnrTG
Cuts at: 0 97 1062
Size: 97 965
MspA1I CmG'CkG
Cuts at: 0 666 751 764 811 912 1062
Size: 666 85 13 47 101 150
Fragments arranged by size:
666 150 101 85 47 13
NaeI GCC'GGC
Cuts at: 0 48 453 624 1002 1062
Size: 48 405 171 378 60
Fragments arranged by size:
405 378 171 60 48
NarI GG'CG_CC
Cuts at: 0 57 680 767 1062
Size: 57 623 87 295
Fragments arranged by size:
623 295 87 57
NcoI C'CATG_G
Cuts at: 0 974 1062
Size: 974 88
NgoMIV G'CCGG_C
Cuts at: 0 46 451 622 1000 1062
Size: 46 405 171 378 62
Fragments arranged by size:
405 378 171 62 46
NotI GC'GGCC_GC
Cuts at: 0 343 1062
Size: 343 719
NspI r_CATG'y
Cuts at: 0 939 1062
Size: 939 123
PfoI T'CCnGG_A
Cuts at: 0 422 1062
Size: 422 640
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 301 333 1062
Size: 301 32 729
Fragments arranged by size:
729 301 32
PstI C_TGCA'G
Cuts at: 0 161 1062
Size: 161 901
SacII CC_GC'GG
Cuts at: 0 752 812 913 1062
Size: 752 60 101 149
Fragments arranged by size:
752 149 101 60
SfcI C'TryA_G
Cuts at: 0 157 1062
Size: 157 905
SfoI GGC'GCC
Cuts at: 0 58 681 768 1062
Size: 58 623 87 294
Fragments arranged by size:
623 294 87 58
SgrAI Cr'CCGG_yG
Cuts at: 0 676 682 1062
Size: 676 6 380
Fragments arranged by size:
676 380 6
SmlI C'TyrA_G
Cuts at: 0 376 1062
Size: 376 686
StyI C'CwwG_G
Cuts at: 0 26 254 261 974 1062
Size: 26 228 7 713 88
Fragments arranged by size:
713 228 88 26 7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 132 959 1062
Size: 132 827 103
Fragments arranged by size:
827 132 103
TatI w'GTAC_w
Cuts at: 0 386 1062
Size: 386 676
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 559 591 1062
Size: 559 32 471
Fragments arranged by size:
559 471 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 846 1062
Size: 846 216
Enzymes that do cut:
AarI AccI AhdI AlfI AlwNI AvaI BanI BanII
BbeI BbsI BcgI BglII BlpI Bme1580I BplI BpmI
BpuEI BsaHI BsaXI BseRI BseYI BsgI BsiEI Bsp1286I
BspMI BsrFI BsrGI BssSI BstEII BstXI BstYI BtgI
BtgZI DraIII EaeI EagI EarI Eco57MI EcoO109I HaeII
Hin4I KasI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NotI NspI PfoI PpiI PstI SacII
SfcI SfoI SgrAI SmlI StyI TaqII TatI TstI
XcmI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AflIII AgeI
AleI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbvCI BciVI BclI BfrBI BglI
BmgBI BmrI BmtI Bpu10I BsaI BsaAI BsaBI BsaWI
BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI
BssHII BstAPI BstBI BstZ17I Bsu36I BtsI ClaI CspCI
DraI DrdI EciI EcoICRI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI HincII HindIII HpaI KpnI MfeI
MluI NdeI NheI NruI NsiI PacI PasI PciI
PflMI PmeI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfiI SgfI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI Tth111I XbaI XhoI
XmaI XmnI ZraI