Restriction map for
pF1KB8963
(Linear) (Six-base) MAPSORT of: KIBB8963.seq Check: 6336 from: 1 to: 1050
KIBB8963.orf
With 182 enzymes: SgfI *
August 7, 2008 14:13 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 684 1050
Size: 684 366
AleI CACnn'nnGTG
Cuts at: 0 112 1050
Size: 112 938
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 37 1050
Size: 37 1013
ApaLI G'TGCA_C
Cuts at: 0 528 1050
Size: 528 522
ApoI r'AATT_y
Cuts at: 0 705 900 1050
Size: 705 195 150
Fragments arranged by size:
705 195 150
AvaI C'yCGr_G
Cuts at: 0 513 1050
Size: 513 537
AvrII C'CTAG_G
Cuts at: 0 808 1050
Size: 808 242
BamHI G'GATC_C
Cuts at: 0 632 812 1050
Size: 632 180 238
Fragments arranged by size:
632 238 180
BanI G'GyrC_C
Cuts at: 0 804 1050
Size: 804 246
BanII G_rGCy'C
Cuts at: 0 756 1050
Size: 756 294
BclI T'GATC_A
Cuts at: 0 599 1050
Size: 599 451
BglI GCCn_nnn'nGGC
Cuts at: 0 72 1050
Size: 72 978
BlpI GC'TnA_GC
Cuts at: 0 204 1050
Size: 204 846
Bme1580I G_kGCm'C
Cuts at: 0 532 1050
Size: 532 518
BmgBI CAC'GTC
Cuts at: 0 978 1050
Size: 978 72
BmrI ACTGGGnnnn_n'
Cuts at: 0 534 1050
Size: 534 516
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 487 628 1050
Size: 487 141 422
Fragments arranged by size:
487 422 141
Bpu10I CC'TnA_GC
Cuts at: 0 172 325 1050
Size: 172 153 725
Fragments arranged by size:
725 172 153
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 382 1050
Size: 382 668
BsaAI yAC'GTr
Cuts at: 0 441 1050
Size: 441 609
BsaHI Gr'CG_yC
Cuts at: 0 79 1050
Size: 79 971
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 436 746 1050
Size: 436 310 304
Fragments arranged by size:
436 310 304
BseYI C'CCAG_C
Cuts at: 0 35 749 1050
Size: 35 714 301
Fragments arranged by size:
714 301 35
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 277 512 1050
Size: 277 235 538
Fragments arranged by size:
538 277 235
BsiEI CG_ry'CG
Cuts at: 0 473 1050
Size: 473 577
BsiHKAI G_wGCw'C
Cuts at: 0 47 446 532 1050
Size: 47 399 86 518
Fragments arranged by size:
518 399 86 47
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1010 1050
Size: 1010 40
Bsp1286I G_dGCh'C
Cuts at: 0 47 446 532 756 1050
Size: 47 399 86 224 294
Fragments arranged by size:
399 294 224 86 47
BsrFI r'CCGG_y
Cuts at: 0 355 1050
Size: 355 695
BsrGI T'GTAC_A
Cuts at: 0 413 940 1050
Size: 413 527 110
Fragments arranged by size:
527 413 110
BssHII G'CGCG_C
Cuts at: 0 17 59 1050
Size: 17 42 991
Fragments arranged by size:
991 42 17
BssSI C'ACGA_G
Cuts at: 0 69 525 1050
Size: 69 456 525
Fragments arranged by size:
525 456 69
BstXI CCAn_nnnn'nTGG
Cuts at: 0 117 307 570 723 1050
Size: 117 190 263 153 327
Fragments arranged by size:
327 263 190 153 117
BstYI r'GATC_y
Cuts at: 0 518 632 812 1050
Size: 518 114 180 238
Fragments arranged by size:
518 238 180 114
BtgI C'CryG_G
Cuts at: 0 48 569 779 1050
Size: 48 521 210 271
Fragments arranged by size:
521 271 210 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 345 549 1050
Size: 345 204 501
Fragments arranged by size:
501 345 204
BtsI GCAGTG_nn'
Cuts at: 0 294 1050
Size: 294 756
EaeI y'GGCC_r
Cuts at: 0 227 470 776 833 980 1050
Size: 227 243 306 57 147 70
Fragments arranged by size:
306 243 227 147 70 57
EagI C'GGCC_G
Cuts at: 0 470 1050
Size: 470 580
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 72 543 1050
Size: 72 471 507
Fragments arranged by size:
507 471 72
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 487 628 684 1050
Size: 487 141 56 366
Fragments arranged by size:
487 366 141 56
MscI TGG'CCA
Cuts at: 0 229 835 1050
Size: 229 606 215
Fragments arranged by size:
606 229 215
MslI CAynn'nnrTG
Cuts at: 0 112 115 193 568 721 1050
Size: 112 3 78 375 153 329
Fragments arranged by size:
375 329 153 112 78 3
MspA1I CmG'CkG
Cuts at: 0 50 863 1050
Size: 50 813 187
Fragments arranged by size:
813 187 50
NdeI CA'TA_TG
Cuts at: 0 451 1050
Size: 451 599
PflMI CCAn_nnn'nTGG
Cuts at: 0 749 1050
Size: 749 301
PvuII CAG'CTG
Cuts at: 0 863 1050
Size: 863 187
SacII CC_GC'GG
Cuts at: 0 51 1050
Size: 51 999
SexAI A'CCwGG_T
Cuts at: 0 218 1050
Size: 218 832
SmlI C'TyrA_G
Cuts at: 0 397 1050
Size: 397 653
StyI C'CwwG_G
Cuts at: 0 808 1050
Size: 808 242
TatI w'GTAC_w
Cuts at: 0 413 940 1050
Size: 413 527 110
Fragments arranged by size:
527 413 110
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 570 843 1050
Size: 570 273 207
Fragments arranged by size:
570 273 207
Enzymes that do cut:
AcuI AleI AlfI ApaLI ApoI AvaI AvrII BamHI
BanI BanII BclI BglI BlpI Bme1580I BmgBI BmrI
BpmI Bpu10I BpuEI BsaAI BsaHI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BsrFI BsrGI BssHII BssSI
BstXI BstYI BtgI BtgZI BtsI EaeI EagI EciI
Eco57MI MscI MslI MspA1I NdeI PflMI PvuII SacII
SexAI SmlI StyI TatI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AloI AlwNI ApaI AscI AseI AsiSI
BaeI BbeI BbsI BbvCI BcgI BciVI BfrBI BglII
BmtI BplI BsaI BsaBI BsaWI BsaXI BsiWI BsmI
BspEI BspHI BspMI BsrBI BsrDI BstAPI BstBI BstEII
BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EarI
EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI
FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI NaeI NarI NcoI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI RsrII SacI SalI SanDI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII
TstI Tth111I XbaI XhoI XmaI XmnI ZraI