Restriction map for
pF1KB8960
(Linear) (Six-base) MAPSORT of: KIBB8960.seq Check: 5520 from: 1 to: 1020
KIBB8960.orf
With 182 enzymes: SgfI *
March 3, 2008 11:09 ..
AccI GT'mk_AC
Cuts at: 0 233 1020
Size: 233 787
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 121 1020
Size: 121 899
AlwNI CAG_nnn'CTG
Cuts at: 0 62 399 1020
Size: 62 337 621
Fragments arranged by size:
621 337 62
AvaI C'yCGr_G
Cuts at: 0 655 1020
Size: 655 365
BamHI G'GATC_C
Cuts at: 0 986 1020
Size: 986 34
BanI G'GyrC_C
Cuts at: 0 809 1020
Size: 809 211
BanII G_rGCy'C
Cuts at: 0 611 629 655 1020
Size: 611 18 26 365
Fragments arranged by size:
611 365 26 18
BbvCI CC'TCA_GC
Cuts at: 0 698 1020
Size: 698 322
BclI T'GATC_A
Cuts at: 0 53 1020
Size: 53 967
BglI GCCn_nnn'nGGC
Cuts at: 0 512 1020
Size: 512 508
BglII A'GATC_T
Cuts at: 0 256 1020
Size: 256 764
Bme1580I G_kGCm'C
Cuts at: 0 814 1020
Size: 814 206
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 517 1020
Size: 517 503
Bpu10I CC'TnA_GC
Cuts at: 0 698 1020
Size: 698 322
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 212 946 1020
Size: 212 734 74
Fragments arranged by size:
734 212 74
BsaI GGTCTCn'nnnn_
Cuts at: 0 708 1020
Size: 708 312
BsaWI w'CCGG_w
Cuts at: 0 983 1020
Size: 983 37
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 60 90 1020
Size: 60 30 930
Fragments arranged by size:
930 60 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 699 1020
Size: 699 321
BseYI C'CCAG_C
Cuts at: 0 578 1020
Size: 578 442
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 745 1020
Size: 745 275
BsiEI CG_ry'CG
Cuts at: 0 377 1020
Size: 377 643
BsmI GAATG_Cn'
Cuts at: 0 146 1020
Size: 146 874
BsmBI CGTCTCn'nnnn_
Cuts at: 0 170 1020
Size: 170 850
Bsp1286I G_dGCh'C
Cuts at: 0 611 629 655 814 1020
Size: 611 18 26 159 206
Fragments arranged by size:
611 206 159 26 18
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 915 1020
Size: 915 105
BsrBI CCG'CTC
Cuts at: 0 617 1020
Size: 617 403
BsrGI T'GTAC_A
Cuts at: 0 101 1020
Size: 101 919
BssSI C'ACGA_G
Cuts at: 0 545 1020
Size: 545 475
BstYI r'GATC_y
Cuts at: 0 246 256 986 1020
Size: 246 10 730 34
Fragments arranged by size:
730 246 34 10
Bsu36I CC'TnA_GG
Cuts at: 0 431 1020
Size: 431 589
BtsI GCAGTG_nn'
Cuts at: 0 782 1020
Size: 782 238
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 528 563 1020
Size: 528 35 457
Fragments arranged by size:
528 457 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 925 1020
Size: 925 95
EaeI y'GGCC_r
Cuts at: 0 170 374 554 618 1020
Size: 170 204 180 64 402
Fragments arranged by size:
402 204 180 170 64
EagI C'GGCC_G
Cuts at: 0 374 1020
Size: 374 646
EarI CTCTTCn'nnn_
Cuts at: 0 674 799 1020
Size: 674 125 221
Fragments arranged by size:
674 221 125
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 517 1020
Size: 517 503
EcoNI CCTnn'n_nnAGG
Cuts at: 0 697 1020
Size: 697 323
EcoO109I rG'GnC_Cy
Cuts at: 0 131 381 1020
Size: 131 250 639
Fragments arranged by size:
639 250 131
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 688 1020
Size: 688 332
HaeII r_GCGC'y
Cuts at: 0 95 885 1020
Size: 95 790 135
Fragments arranged by size:
790 135 95
HincII GTy'rAC
Cuts at: 0 909 1020
Size: 909 111
HpaI GTT'AAC
Cuts at: 0 909 1020
Size: 909 111
MfeI C'AATT_G
Cuts at: 0 333 774 1020
Size: 333 441 246
Fragments arranged by size:
441 333 246
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 1020
Size: 220 800
MscI TGG'CCA
Cuts at: 0 172 1020
Size: 172 848
MslI CAynn'nnrTG
Cuts at: 0 103 526 1020
Size: 103 423 494
Fragments arranged by size:
494 423 103
MspA1I CmG'CkG
Cuts at: 0 574 582 1020
Size: 574 8 438
Fragments arranged by size:
574 438 8
NspI r_CATG'y
Cuts at: 0 102 108 345 1020
Size: 102 6 237 675
Fragments arranged by size:
675 237 102 6
PflMI CCAn_nnn'nTGG
Cuts at: 0 198 399 776 834 1020
Size: 198 201 377 58 186
Fragments arranged by size:
377 201 198 186 58
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 841 873 1020
Size: 841 32 147
Fragments arranged by size:
841 147 32
PpuMI rG'GwC_Cy
Cuts at: 0 131 381 1020
Size: 131 250 639
Fragments arranged by size:
639 250 131
PstI C_TGCA'G
Cuts at: 0 67 587 1020
Size: 67 520 433
Fragments arranged by size:
520 433 67
PvuII CAG'CTG
Cuts at: 0 582 1020
Size: 582 438
SbfI CC_TGCA'GG
Cuts at: 0 67 1020
Size: 67 953
SfcI C'TryA_G
Cuts at: 0 63 583 1020
Size: 63 520 437
Fragments arranged by size:
520 437 63
SmlI C'TyrA_G
Cuts at: 0 227 961 1020
Size: 227 734 59
Fragments arranged by size:
734 227 59
TatI w'GTAC_w
Cuts at: 0 101 494 1020
Size: 101 393 526
Fragments arranged by size:
526 393 101
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 275 307 1020
Size: 275 32 713
Fragments arranged by size:
713 275 32
Tth111I GACn'n_nGTC
Cuts at: 0 461 1020
Size: 461 559
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 773 1020
Size: 773 247
Enzymes that do cut:
AccI AlfI AlwNI AvaI BamHI BanI BanII BbvCI
BclI BglI BglII Bme1580I BpmI Bpu10I BpuEI BsaI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsmI BsmBI
Bsp1286I BspMI BsrBI BsrGI BssSI BstYI Bsu36I BtsI
CspCI DrdI EaeI EagI EarI Eco57MI EcoNI EcoO109I
FalI HaeII HincII HpaI MfeI MmeI MscI MslI
MspA1I NspI PflMI PpiI PpuMI PstI PvuII SbfI
SfcI SmlI TatI TstI Tth111I XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BbeI BbsI BcgI BciVI
BfrBI BlpI BmgBI BmrI BmtI BplI BsaAI BsaBI
BsaHI BsiHKAI BsiWI BspEI BspHI BsrDI BsrFI BssHII
BstAPI BstBI BstEII BstXI BstZ17I BtgI BtgZI ClaI
DraI DraIII EciI EcoICRI EcoRI EcoRV FseI FspI
FspAI Hin4I HindIII KasI KpnI MluI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PfoI PmeI PmlI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SapI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI StyI SwaI
TaqII XbaI XhoI XmaI XmnI ZraI