Restriction map for
pF1KB8959
(Linear) (Six-base) MAPSORT of: KIBB8959.seq Check: 2576 from: 1 to: 1014
KIBB8959.orf
With 182 enzymes: SgfI *
March 3, 2008 11:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 470 1014
Size: 470 544
AfeI AGC'GCT
Cuts at: 0 577 1014
Size: 577 437
AhdI GACnn_n'nnGTC
Cuts at: 0 540 1014
Size: 540 474
ApaLI G'TGCA_C
Cuts at: 0 331 1014
Size: 331 683
ApoI r'AATT_y
Cuts at: 0 58 1014
Size: 58 956
AvaI C'yCGr_G
Cuts at: 0 865 1014
Size: 865 149
BanI G'GyrC_C
Cuts at: 0 448 846 1014
Size: 448 398 168
Fragments arranged by size:
448 398 168
BanII G_rGCy'C
Cuts at: 0 664 803 1014
Size: 664 139 211
Fragments arranged by size:
664 211 139
BbeI G_GCGC'C
Cuts at: 0 452 850 1014
Size: 452 398 164
Fragments arranged by size:
452 398 164
BbvCI CC'TCA_GC
Cuts at: 0 529 1014
Size: 529 485
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 822 856 1014
Size: 822 34 158
Fragments arranged by size:
822 158 34
BglI GCCn_nnn'nGGC
Cuts at: 0 347 1014
Size: 347 667
BlpI GC'TnA_GC
Cuts at: 0 667 1014
Size: 667 347
Bme1580I G_kGCm'C
Cuts at: 0 260 287 335 1014
Size: 260 27 48 679
Fragments arranged by size:
679 260 48 27
BmgBI CAC'GTC
Cuts at: 0 266 537 621 836 892 1014
Size: 266 271 84 215 56 122
Fragments arranged by size:
271 266 215 122 84 56
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 121 384 1014
Size: 121 263 630
Fragments arranged by size:
630 263 121
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 48 379 727 1014
Size: 48 331 348 287
Fragments arranged by size:
348 331 287 48
Bpu10I CC'TnA_GC
Cuts at: 0 529 1014
Size: 529 485
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 628 715 1014
Size: 628 87 299
Fragments arranged by size:
628 299 87
BsaHI Gr'CG_yC
Cuts at: 0 230 449 847 1014
Size: 230 219 398 167
Fragments arranged by size:
398 230 219 167
BsaWI w'CCGG_w
Cuts at: 0 402 1014
Size: 402 612
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 64 94 1014
Size: 64 30 920
Fragments arranged by size:
920 64 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 772 1014
Size: 772 242
BseYI C'CCAG_C
Cuts at: 0 108 297 600 1014
Size: 108 189 303 414
Fragments arranged by size:
414 303 189 108
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 246 448 1014
Size: 55 191 202 566
Fragments arranged by size:
566 202 191 55
BsiHKAI G_wGCw'C
Cuts at: 0 335 365 743 803 1014
Size: 335 30 378 60 211
Fragments arranged by size:
378 335 211 60 30
BsmBI CGTCTCn'nnnn_
Cuts at: 0 201 1014
Size: 201 813
Bsp1286I G_dGCh'C
Cuts at: 0 260 287 335 365 664 743 803 1014
Size: 260 27 48 30 299 79 60 211
Fragments arranged by size:
299 260 211 79 60 48 30 27
BspEI T'CCGG_A
Cuts at: 0 402 1014
Size: 402 612
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 362 470 677 1014
Size: 362 108 207 337
Fragments arranged by size:
362 337 207 108
BsrBI CCG'CTC
Cuts at: 0 389 1014
Size: 389 625
BsrFI r'CCGG_y
Cuts at: 0 735 1014
Size: 735 279
BstAPI GCAn_nnn'nTGC
Cuts at: 0 42 683 1014
Size: 42 641 331
Fragments arranged by size:
641 331 42
BstEII G'GTnAC_C
Cuts at: 0 606 1014
Size: 606 408
BstYI r'GATC_y
Cuts at: 0 428 647 1014
Size: 428 219 367
Fragments arranged by size:
428 367 219
BtgI C'CryG_G
Cuts at: 0 201 452 473 497 1014
Size: 201 251 21 24 517
Fragments arranged by size:
517 251 201 24 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 825 903 1014
Size: 825 78 111
Fragments arranged by size:
825 111 78
DraIII CAC_nnn'GTG
Cuts at: 0 42 330 414 498 1014
Size: 42 288 84 84 516
Fragments arranged by size:
516 288 84 84 42
EaeI y'GGCC_r
Cuts at: 0 5 914 1014
Size: 5 909 100
Fragments arranged by size:
909 100 5
EarI CTCTTCn'nnn_
Cuts at: 0 808 1014
Size: 808 206
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 631 1014
Size: 631 383
EcoICRI GAG'CTC
Cuts at: 0 801 1014
Size: 801 213
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 48 379 727 1014
Size: 48 331 348 287
Fragments arranged by size:
348 331 287 48
EcoNI CCTnn'n_nnAGG
Cuts at: 0 466 1014
Size: 466 548
EcoO109I rG'GnC_Cy
Cuts at: 0 339 1014
Size: 339 675
EcoRV GAT'ATC
Cuts at: 0 192 1014
Size: 192 822
HaeII r_GCGC'y
Cuts at: 0 401 452 515 579 850 1014
Size: 401 51 63 64 271 164
Fragments arranged by size:
401 271 164 64 63 51
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 181 213 526 558 1014
Size: 181 32 313 32 456
Fragments arranged by size:
456 313 181 32 32
HindIII A'AGCT_T
Cuts at: 0 141 1014
Size: 141 873
KasI G'GCGC_C
Cuts at: 0 448 846 1014
Size: 448 398 168
Fragments arranged by size:
448 398 168
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 734 1014
Size: 734 280
MscI TGG'CCA
Cuts at: 0 7 1014
Size: 7 1007
MslI CAynn'nnrTG
Cuts at: 0 478 1014
Size: 478 536
MspA1I CmG'CkG
Cuts at: 0 120 301 588 1014
Size: 120 181 287 426
Fragments arranged by size:
426 287 181 120
NarI GG'CG_CC
Cuts at: 0 449 847 1014
Size: 449 398 167
Fragments arranged by size:
449 398 167
NcoI C'CATG_G
Cuts at: 0 452 473 1014
Size: 452 21 541
Fragments arranged by size:
541 452 21
NspI r_CATG'y
Cuts at: 0 36 527 1014
Size: 36 491 487
Fragments arranged by size:
491 487 36
PflMI CCAn_nnn'nTGG
Cuts at: 0 474 1014
Size: 474 540
PspXI vC'TCGA_Gb
Cuts at: 0 865 1014
Size: 865 149
PstI C_TGCA'G
Cuts at: 0 125 688 1014
Size: 125 563 326
Fragments arranged by size:
563 326 125
PvuII CAG'CTG
Cuts at: 0 120 301 1014
Size: 120 181 713
Fragments arranged by size:
713 181 120
SacI G_AGCT'C
Cuts at: 0 803 1014
Size: 803 211
SapI GCTCTTCn'nnn_
Cuts at: 0 808 1014
Size: 808 206
SfcI C'TryA_G
Cuts at: 0 121 684 1014
Size: 121 563 330
Fragments arranged by size:
563 330 121
SfoI GGC'GCC
Cuts at: 0 450 848 1014
Size: 450 398 166
Fragments arranged by size:
450 398 166
SmlI C'TyrA_G
Cuts at: 0 643 730 865 1014
Size: 643 87 135 149
Fragments arranged by size:
643 149 135 87
SphI G_CATG'C
Cuts at: 0 36 527 1014
Size: 36 491 487
Fragments arranged by size:
491 487 36
StuI AGG'CCT
Cuts at: 0 748 871 1014
Size: 748 123 143
Fragments arranged by size:
748 143 123
StyI C'CwwG_G
Cuts at: 0 452 473 1014
Size: 452 21 541
Fragments arranged by size:
541 452 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 311 1014
Size: 311 703
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 64 96 1014
Size: 64 32 918
Fragments arranged by size:
918 64 32
XhoI C'TCGA_G
Cuts at: 0 865 1014
Size: 865 149
Enzymes that do cut:
AarI AfeI AhdI ApaLI ApoI AvaI BanI BanII
BbeI BbvCI BcgI BglI BlpI Bme1580I BmgBI BplI
BpmI Bpu10I BpuEI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI BsrFI
BstAPI BstEII BstYI BtgI BtgZI DraIII EaeI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV HaeII Hin4I
HindIII KasI MmeI MscI MslI MspA1I NarI NcoI
NspI PflMI PspXI PstI PvuII SacI SapI SfcI
SfoI SmlI SphI StuI StyI TaqII TstI XhoI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AleI AlfI AloI AlwNI ApaI AscI AseI AsiSI
AvrII BaeI BamHI BbsI BciVI BclI BfrBI BglII
BmrI BmtI BsaI BsaAI BsaBI BsiEI BsiWI BsmI
BspHI BsrDI BsrGI BssHII BssSI BstBI BstXI BstZ17I
Bsu36I BtsI ClaI CspCI DraI DrdI EagI EcoRI
FalI FseI FspI FspAI HincII HpaI KpnI MfeI
MluI NaeI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PsrI PvuI RsrII SacII SalI
SanDI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI
SnaBI SpeI SrfI SspI SwaI TatI Tth111I XbaI
XcmI XmaI XmnI ZraI