Restriction map for
pF1KB8958
(Linear) (Six-base) MAPSORT of: KIBB8958.seq Check: 9557 from: 1 to: 1005
KIBB8958.orf
With 182 enzymes: SgfI *
March 3, 2008 11:09 ..
Acc65I G'GTAC_C
Cuts at: 0 365 1005
Size: 365 640
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 294 1005
Size: 294 711
AflIII A'CryG_T
Cuts at: 0 332 1005
Size: 332 673
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 190 1005
Size: 190 815
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 724 756 1005
Size: 724 32 249
Fragments arranged by size:
724 249 32
AlwNI CAG_nnn'CTG
Cuts at: 0 510 1005
Size: 510 495
AvaI C'yCGr_G
Cuts at: 0 57 74 966 1005
Size: 57 17 892 39
Fragments arranged by size:
892 57 39 17
BanI G'GyrC_C
Cuts at: 0 365 1005
Size: 365 640
BanII G_rGCy'C
Cuts at: 0 466 1005
Size: 466 539
BbsI GAAGACnn'nnnn_
Cuts at: 0 965 1005
Size: 965 40
BglII A'GATC_T
Cuts at: 0 821 1005
Size: 821 184
BlpI GC'TnA_GC
Cuts at: 0 69 1005
Size: 69 936
BmrI ACTGGGnnnn_n'
Cuts at: 0 366 1005
Size: 366 639
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 945 1005
Size: 945 60
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 584 751 1005
Size: 584 167 254
Fragments arranged by size:
584 254 167
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 261 1005
Size: 261 744
BsaI GGTCTCn'nnnn_
Cuts at: 0 802 877 1005
Size: 802 75 128
Fragments arranged by size:
802 128 75
BsaAI yAC'GTr
Cuts at: 0 524 1005
Size: 524 481
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 582 612 724 754 1005
Size: 582 30 112 30 251
Fragments arranged by size:
582 251 112 30 30
BseYI C'CCAG_C
Cuts at: 0 224 448 1005
Size: 224 224 557
Fragments arranged by size:
557 224 224
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 1005
Size: 196 809
BsiEI CG_ry'CG
Cuts at: 0 153 1005
Size: 153 852
BsiHKAI G_wGCw'C
Cuts at: 0 503 1005
Size: 503 502
Bsp1286I G_dGCh'C
Cuts at: 0 466 503 1005
Size: 466 37 502
Fragments arranged by size:
502 466 37
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 73 1005
Size: 73 932
BsrFI r'CCGG_y
Cuts at: 0 228 1005
Size: 228 777
BssHII G'CGCG_C
Cuts at: 0 765 1005
Size: 765 240
BstXI CCAn_nnnn'nTGG
Cuts at: 0 490 1005
Size: 490 515
BstYI r'GATC_y
Cuts at: 0 821 1005
Size: 821 184
BtgI C'CryG_G
Cuts at: 0 413 1005
Size: 413 592
BtsI GCAGTG_nn'
Cuts at: 0 54 1005
Size: 54 951
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 262 297 1005
Size: 262 35 708
Fragments arranged by size:
708 262 35
EaeI y'GGCC_r
Cuts at: 0 890 1005
Size: 890 115
EarI CTCTTCn'nnn_
Cuts at: 0 951 1005
Size: 951 54
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 294 584 751 1005
Size: 294 290 167 254
Fragments arranged by size:
294 290 254 167
EcoO109I rG'GnC_Cy
Cuts at: 0 62 1005
Size: 62 943
FspI TGC'GCA
Cuts at: 0 697 1005
Size: 697 308
FspAI rTGC'GCAy
Cuts at: 0 697 1005
Size: 697 308
HaeII r_GCGC'y
Cuts at: 0 131 1005
Size: 131 874
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 582 614 1005
Size: 582 32 391
Fragments arranged by size:
582 391 32
HincII GTy'rAC
Cuts at: 0 682 1005
Size: 682 323
KpnI G_GTAC'C
Cuts at: 0 369 1005
Size: 369 636
MluI A'CGCG_T
Cuts at: 0 332 1005
Size: 332 673
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 639 1005
Size: 639 366
MslI CAynn'nnrTG
Cuts at: 0 488 1005
Size: 488 517
MspA1I CmG'CkG
Cuts at: 0 385 1005
Size: 385 620
NaeI GCC'GGC
Cuts at: 0 230 1005
Size: 230 775
NcoI C'CATG_G
Cuts at: 0 413 1005
Size: 413 592
NdeI CA'TA_TG
Cuts at: 0 489 1005
Size: 489 516
NgoMIV G'CCGG_C
Cuts at: 0 228 1005
Size: 228 777
PflMI CCAn_nnn'nTGG
Cuts at: 0 295 510 1005
Size: 295 215 495
Fragments arranged by size:
495 295 215
PfoI T'CCnGG_A
Cuts at: 0 26 247 1005
Size: 26 221 758
Fragments arranged by size:
758 221 26
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 724 756 1005
Size: 724 32 249
Fragments arranged by size:
724 249 32
PpuMI rG'GwC_Cy
Cuts at: 0 62 1005
Size: 62 943
PstI C_TGCA'G
Cuts at: 0 800 1005
Size: 800 205
SapI GCTCTTCn'nnn_
Cuts at: 0 951 1005
Size: 951 54
SfcI C'TryA_G
Cuts at: 0 796 1005
Size: 796 209
SmaI CCC'GGG
Cuts at: 0 968 1005
Size: 968 37
SmlI C'TyrA_G
Cuts at: 0 276 1005
Size: 276 729
SnaBI TAC'GTA
Cuts at: 0 524 1005
Size: 524 481
StyI C'CwwG_G
Cuts at: 0 413 1005
Size: 413 592
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 280 445 1005
Size: 280 165 560
Fragments arranged by size:
560 280 165
XmaI C'CCGG_G
Cuts at: 0 966 1005
Size: 966 39
XmnI GAAnn'nnTTC
Cuts at: 0 22 739 1005
Size: 22 717 266
Fragments arranged by size:
717 266 22
Enzymes that do cut:
Acc65I AcuI AflIII AlfI AloI AlwNI AvaI BanI
BanII BbsI BglII BlpI BmrI BplI BpmI BpuEI
BsaI BsaAI BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspMI BsrFI BssHII BstXI BstYI BtgI BtsI CspCI
EaeI EarI Eco57MI EcoO109I FspI FspAI HaeII Hin4I
HincII KpnI MluI MmeI MslI MspA1I NaeI NcoI
NdeI NgoMIV PflMI PfoI PpiI PpuMI PstI SapI
SfcI SmaI SmlI SnaBI StyI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AhdI
AleI ApaI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbvCI BcgI BciVI BclI BfrBI
BglI Bme1580I BmgBI BmtI Bpu10I BsaBI BsaHI BsaWI
BseRI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI
BsrGI BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI
ClaI DraI DraIII DrdI EagI EciI EcoICRI EcoNI
EcoRI EcoRV FalI FseI HindIII HpaI KasI MfeI
MscI NarI NheI NotI NruI NsiI NspI PacI
PasI PciI PmeI PmlI PshAI PsiI PspOMI PspXI
PsrI PvuI PvuII RsrII SacI SacII SalI SanDI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SpeI
SphI SrfI SspI StuI SwaI TaqII TatI TstI
Tth111I XbaI XhoI ZraI