Restriction map for
pF1KB8955
(Linear) (Six-base) MAPSORT of: KIBB8955.seq Check: 2862 from: 1 to: 996
KIBB8955.orf
With 182 enzymes: SgfI *
March 3, 2008 11:09 ..
AccI GT'mk_AC
Cuts at: 0 302 996
Size: 302 694
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 264 622 996
Size: 264 358 374
Fragments arranged by size:
374 358 264
AvaI C'yCGr_G
Cuts at: 0 359 663 996
Size: 359 304 333
Fragments arranged by size:
359 333 304
BanII G_rGCy'C
Cuts at: 0 173 274 996
Size: 173 101 722
Fragments arranged by size:
722 173 101
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 93 127 996
Size: 93 34 869
Fragments arranged by size:
869 93 34
BglI GCCn_nnn'nGGC
Cuts at: 0 160 996
Size: 160 836
BglII A'GATC_T
Cuts at: 0 611 996
Size: 611 385
Bme1580I G_kGCm'C
Cuts at: 0 100 996
Size: 100 896
BmgBI CAC'GTC
Cuts at: 0 159 996
Size: 159 837
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 58 661 900 996
Size: 28 30 603 239 96
Fragments arranged by size:
603 239 96 30 28
Bpu10I CC'TnA_GC
Cuts at: 0 331 785 996
Size: 331 454 211
Fragments arranged by size:
454 331 211
BsaAI yAC'GTr
Cuts at: 0 30 996
Size: 30 966
BsaHI Gr'CG_yC
Cuts at: 0 118 996
Size: 118 878
BsaWI w'CCGG_w
Cuts at: 0 875 996
Size: 875 121
BseYI C'CCAG_C
Cuts at: 0 964 996
Size: 964 32
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 198 444 996
Size: 198 246 552
Fragments arranged by size:
552 246 198
BsiEI CG_ry'CG
Cuts at: 0 155 996
Size: 155 841
BsiHKAI G_wGCw'C
Cuts at: 0 263 274 996
Size: 263 11 722
Fragments arranged by size:
722 263 11
Bsp1286I G_dGCh'C
Cuts at: 0 100 173 263 274 996
Size: 100 73 90 11 722
Fragments arranged by size:
722 100 90 73 11
BsrBI CCG'CTC
Cuts at: 0 330 996
Size: 330 666
BsrDI GCAATG_nn'
Cuts at: 0 374 818 996
Size: 374 444 178
Fragments arranged by size:
444 374 178
BstEII G'GTnAC_C
Cuts at: 0 740 996
Size: 740 256
BstXI CCAn_nnnn'nTGG
Cuts at: 0 204 705 717 996
Size: 204 501 12 279
Fragments arranged by size:
501 279 204 12
BstYI r'GATC_y
Cuts at: 0 611 686 996
Size: 611 75 310
Fragments arranged by size:
611 310 75
BtgI C'CryG_G
Cuts at: 0 89 164 293 996
Size: 89 75 129 703
Fragments arranged by size:
703 129 89 75
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 33 996
Size: 33 963
BtsI GCAGTG_nn'
Cuts at: 0 580 996
Size: 580 416
EaeI y'GGCC_r
Cuts at: 0 152 161 290 314 707 821 996
Size: 152 9 129 24 393 114 175
Fragments arranged by size:
393 175 152 129 114 24 9
EagI C'GGCC_G
Cuts at: 0 152 996
Size: 152 844
EarI CTCTTCn'nnn_
Cuts at: 0 178 241 996
Size: 178 63 755
Fragments arranged by size:
755 178 63
EcoICRI GAG'CTC
Cuts at: 0 272 996
Size: 272 724
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 58 264 622 661 900 996
Size: 28 30 206 358 39 239 96
Fragments arranged by size:
358 239 206 96 39 30 28
EcoO109I rG'GnC_Cy
Cuts at: 0 356 390 996
Size: 356 34 606
Fragments arranged by size:
606 356 34
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 531 996
Size: 531 465
HaeII r_GCGC'y
Cuts at: 0 37 996
Size: 37 959
HincII GTy'rAC
Cuts at: 0 303 759 996
Size: 303 456 237
Fragments arranged by size:
456 303 237
HpaI GTT'AAC
Cuts at: 0 759 996
Size: 759 237
MscI TGG'CCA
Cuts at: 0 316 709 823 996
Size: 316 393 114 173
Fragments arranged by size:
393 316 173 114
MslI CAynn'nnrTG
Cuts at: 0 715 996
Size: 715 281
MspA1I CmG'CkG
Cuts at: 0 91 681 996
Size: 91 590 315
Fragments arranged by size:
590 315 91
NspI r_CATG'y
Cuts at: 0 754 841 996
Size: 754 87 155
Fragments arranged by size:
754 155 87
PasI CC'CwG_GG
Cuts at: 0 470 996
Size: 470 526
PpuMI rG'GwC_Cy
Cuts at: 0 356 390 996
Size: 356 34 606
Fragments arranged by size:
606 356 34
PsiI TTA'TAA
Cuts at: 0 589 996
Size: 589 407
PspXI vC'TCGA_Gb
Cuts at: 0 663 996
Size: 663 333
PstI C_TGCA'G
Cuts at: 0 116 631 996
Size: 116 515 365
Fragments arranged by size:
515 365 116
SacI G_AGCT'C
Cuts at: 0 274 996
Size: 274 722
SacII CC_GC'GG
Cuts at: 0 92 996
Size: 92 904
SalI G'TCGA_C
Cuts at: 0 301 996
Size: 301 695
SanDI GG'GwC_CC
Cuts at: 0 356 996
Size: 356 640
SapI GCTCTTCn'nnn_
Cuts at: 0 178 996
Size: 178 818
SbfI CC_TGCA'GG
Cuts at: 0 631 996
Size: 631 365
SfcI C'TryA_G
Cuts at: 0 112 627 996
Size: 112 515 369
Fragments arranged by size:
515 369 112
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 160 996
Size: 160 836
SmlI C'TyrA_G
Cuts at: 0 663 996
Size: 663 333
SphI G_CATG'C
Cuts at: 0 754 996
Size: 754 242
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 554 996
Size: 554 442
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 132 425 540 996
Size: 132 293 115 456
Fragments arranged by size:
456 293 132 115
XhoI C'TCGA_G
Cuts at: 0 663 996
Size: 663 333
Enzymes that do cut:
AccI AcuI AvaI BanII BcgI BglI BglII Bme1580I
BmgBI BpmI Bpu10I BsaAI BsaHI BsaWI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BsrBI BsrDI BstEII BstXI BstYI
BtgI BtgZI BtsI EaeI EagI EarI EcoICRI Eco57MI
EcoO109I FalI HaeII HincII HpaI MscI MslI MspA1I
NspI PasI PpuMI PsiI PspXI PstI SacI SacII
SalI SanDI SapI SbfI SfcI SfiI SmlI SphI
TaqII XcmI XhoI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BanI BbeI
BbsI BbvCI BciVI BclI BfrBI BlpI BmrI BmtI
BplI BpuEI BsaI BsaBI BsaXI BseRI BsiWI BsmI
BsmBI BspEI BspHI BspMI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstZ17I Bsu36I ClaI CspCI DraI DraIII
DrdI EciI EcoNI EcoRI EcoRV FseI FspI FspAI
Hin4I HindIII KasI KpnI MfeI MluI MmeI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PspOMI PsrI PvuI PvuII RsrII ScaI SexAI SfoI
SgfI SgrAI SmaI SnaBI SpeI SrfI SspI StuI
StyI SwaI TatI TstI Tth111I XbaI XmaI XmnI
ZraI