Restriction map for
pF1KB8953
(Linear) (Six-base) MAPSORT of: KIBB8953.seq Check: 2282 from: 1 to: 984
KIBB8953.orf
With 182 enzymes: SgfI *
March 3, 2008 11:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 297 984
Size: 297 687
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 588 984
Size: 588 396
AflIII A'CryG_T
Cuts at: 0 20 984
Size: 20 964
AhdI GACnn_n'nnGTC
Cuts at: 0 384 984
Size: 384 600
AleI CACnn'nnGTG
Cuts at: 0 524 984
Size: 524 460
ApaLI G'TGCA_C
Cuts at: 0 252 984
Size: 252 732
ApoI r'AATT_y
Cuts at: 0 702 984
Size: 702 282
AvaI C'yCGr_G
Cuts at: 0 351 420 764 984
Size: 351 69 344 220
Fragments arranged by size:
351 344 220 69
BanI G'GyrC_C
Cuts at: 0 69 142 280 556 984
Size: 69 73 138 276 428
Fragments arranged by size:
428 276 138 73 69
BanII G_rGCy'C
Cuts at: 0 7 771 984
Size: 7 764 213
Fragments arranged by size:
764 213 7
BbeI G_GCGC'C
Cuts at: 0 146 984
Size: 146 838
BbsI GAAGACnn'nnnn_
Cuts at: 0 408 984
Size: 408 576
BbvCI CC'TCA_GC
Cuts at: 0 442 984
Size: 442 542
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 262 296 984
Size: 262 34 688
Fragments arranged by size:
688 262 34
BglI GCCn_nnn'nGGC
Cuts at: 0 409 984
Size: 409 575
BlpI GC'TnA_GC
Cuts at: 0 61 984
Size: 61 923
Bme1580I G_kGCm'C
Cuts at: 0 256 984
Size: 256 728
BmgBI CAC'GTC
Cuts at: 0 57 393 984
Size: 57 336 591
Fragments arranged by size:
591 336 57
BmrI ACTGGGnnnn_n'
Cuts at: 0 234 984
Size: 234 750
BmtI G_CTAG'C
Cuts at: 0 680 984
Size: 680 304
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 280 669 984
Size: 34 246 389 315
Fragments arranged by size:
389 315 246 34
Bpu10I CC'TnA_GC
Cuts at: 0 442 984
Size: 442 542
BsaI GGTCTCn'nnnn_
Cuts at: 0 392 984
Size: 392 592
BsaAI yAC'GTr
Cuts at: 0 21 984
Size: 21 963
BsaHI Gr'CG_yC
Cuts at: 0 92 143 984
Size: 92 51 841
Fragments arranged by size:
841 92 51
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 7 37 984
Size: 7 30 947
Fragments arranged by size:
947 30 7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 430 984
Size: 430 554
BseYI C'CCAG_C
Cuts at: 0 301 360 984
Size: 301 59 624
Fragments arranged by size:
624 301 59
BsiEI CG_ry'CG
Cuts at: 0 200 214 233 374 413 984
Size: 200 14 19 141 39 571
Fragments arranged by size:
571 200 141 39 19 14
BsiHKAI G_wGCw'C
Cuts at: 0 7 62 256 984
Size: 7 55 194 728
Fragments arranged by size:
728 194 55 7
BsiWI C'GTAC_G
Cuts at: 0 341 984
Size: 341 643
BsmI GAATG_Cn'
Cuts at: 0 642 984
Size: 642 342
BsmBI CGTCTCn'nnnn_
Cuts at: 0 579 984
Size: 579 405
Bsp1286I G_dGCh'C
Cuts at: 0 7 62 256 771 984
Size: 7 55 194 515 213
Fragments arranged by size:
515 213 194 55 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 297 984
Size: 297 687
BsrBI CCG'CTC
Cuts at: 0 86 984
Size: 86 898
BsrFI r'CCGG_y
Cuts at: 0 336 984
Size: 336 648
BstXI CCAn_nnnn'nTGG
Cuts at: 0 894 984
Size: 894 90
BtgI C'CryG_G
Cuts at: 0 101 293 984
Size: 101 192 691
Fragments arranged by size:
691 192 101
BtsI GCAGTG_nn'
Cuts at: 0 256 984
Size: 256 728
DrdI GACnn_nn'nnGTC
Cuts at: 0 814 984
Size: 814 170
EaeI y'GGCC_r
Cuts at: 0 167 197 211 230 371 410 447 516
Size: 167 30 14 19 141 39 37 69
Cuts at: 516 878 984
Size: 362 106
Fragments arranged by size:
362 167 141 106 69 39 37 30
19 14
EagI C'GGCC_G
Cuts at: 0 197 211 230 371 410 984
Size: 197 14 19 141 39 574
Fragments arranged by size:
574 197 141 39 19 14
EarI CTCTTCn'nnn_
Cuts at: 0 625 984
Size: 625 359
EcoICRI GAG'CTC
Cuts at: 0 5 984
Size: 5 979
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 280 588 669 984
Size: 34 246 308 81 315
Fragments arranged by size:
315 308 246 81 34
EcoO109I rG'GnC_Cy
Cuts at: 0 266 984
Size: 266 718
HaeII r_GCGC'y
Cuts at: 0 146 984
Size: 146 838
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 7 39 370 402 984
Size: 7 32 331 32 582
Fragments arranged by size:
582 331 32 32 7
KasI G'GCGC_C
Cuts at: 0 142 984
Size: 142 842
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 113 930 984
Size: 113 817 54
Fragments arranged by size:
817 113 54
MscI TGG'CCA
Cuts at: 0 880 984
Size: 880 104
MslI CAynn'nnrTG
Cuts at: 0 524 803 892 984
Size: 524 279 89 92
Fragments arranged by size:
524 279 92 89
MspA1I CmG'CkG
Cuts at: 0 38 155 295 301 446 984
Size: 38 117 140 6 145 538
Fragments arranged by size:
538 145 140 117 38 6
NaeI GCC'GGC
Cuts at: 0 338 984
Size: 338 646
NarI GG'CG_CC
Cuts at: 0 143 984
Size: 143 841
NgoMIV G'CCGG_C
Cuts at: 0 336 984
Size: 336 648
NheI G'CTAG_C
Cuts at: 0 676 984
Size: 676 308
PspXI vC'TCGA_Gb
Cuts at: 0 764 984
Size: 764 220
PstI C_TGCA'G
Cuts at: 0 113 984
Size: 113 871
PvuII CAG'CTG
Cuts at: 0 155 301 984
Size: 155 146 683
Fragments arranged by size:
683 155 146
SacI G_AGCT'C
Cuts at: 0 7 984
Size: 7 977
SacII CC_GC'GG
Cuts at: 0 296 984
Size: 296 688
ScaI AGT'ACT
Cuts at: 0 757 984
Size: 757 227
SexAI A'CCwGG_T
Cuts at: 0 276 984
Size: 276 708
SfcI C'TryA_G
Cuts at: 0 109 984
Size: 109 875
SfoI GGC'GCC
Cuts at: 0 144 984
Size: 144 840
SmaI CCC'GGG
Cuts at: 0 353 984
Size: 353 631
SmlI C'TyrA_G
Cuts at: 0 764 984
Size: 764 220
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 87 984
Size: 87 897
TatI w'GTAC_w
Cuts at: 0 755 984
Size: 755 229
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 873 905 984
Size: 873 32 79
Fragments arranged by size:
873 79 32
XhoI C'TCGA_G
Cuts at: 0 764 984
Size: 764 220
XmaI C'CCGG_G
Cuts at: 0 351 984
Size: 351 633
XmnI GAAnn'nnTTC
Cuts at: 0 872 984
Size: 872 112
Enzymes that do cut:
AarI AcuI AflIII AhdI AleI ApaLI ApoI AvaI
BanI BanII BbeI BbsI BbvCI BcgI BglI BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BsaI BsaAI
BsaHI BsaXI BseRI BseYI BsiEI BsiHKAI BsiWI BsmI
BsmBI Bsp1286I BspMI BsrBI BsrFI BstXI BtgI BtsI
DrdI EaeI EagI EarI EcoICRI Eco57MI EcoO109I HaeII
Hin4I KasI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NheI PspXI PstI PvuII SacI SacII ScaI
SexAI SfcI SfoI SmaI SmlI TaqII TatI TstI
XhoI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AlfI
AloI AlwNI ApaI AscI AseI AsiSI AvrII BaeI
BamHI BciVI BclI BfrBI BglII BplI BpuEI BsaBI
BsaWI BsgI BspEI BspHI BsrDI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DraIII EciI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI HincII HindIII HpaI KpnI MfeI
MluI NcoI NdeI NotI NruI NsiI NspI PacI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PsrI PvuI RsrII SalI SanDI
SapI SbfI SfiI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI StuI StyI SwaI Tth111I XbaI XcmI
ZraI