Restriction map for
pF1KB8951
(Linear) (Six-base) MAPSORT of: KIBB8951.seq Check: 4797 from: 1 to: 978
KIBB8951.orf
With 182 enzymes: SgfI *
March 3, 2008 11:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 244 978
Size: 244 734
AccI GT'mk_AC
Cuts at: 0 542 978
Size: 542 436
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 709 978
Size: 709 269
AflII C'TTAA_G
Cuts at: 0 898 978
Size: 898 80
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 64 978
Size: 64 914
AlwNI CAG_nnn'CTG
Cuts at: 0 117 978
Size: 117 861
AvaI C'yCGr_G
Cuts at: 0 73 978
Size: 73 905
BamHI G'GATC_C
Cuts at: 0 395 978
Size: 395 583
BanI G'GyrC_C
Cuts at: 0 175 978
Size: 175 803
BanII G_rGCy'C
Cuts at: 0 280 494 978
Size: 280 214 484
Fragments arranged by size:
484 280 214
BbsI GAAGACnn'nnnn_
Cuts at: 0 600 978
Size: 600 378
BbvCI CC'TCA_GC
Cuts at: 0 733 978
Size: 733 245
BclI T'GATC_A
Cuts at: 0 497 978
Size: 497 481
BglI GCCn_nnn'nGGC
Cuts at: 0 295 978
Size: 295 683
Bme1580I G_kGCm'C
Cuts at: 0 74 180 978
Size: 74 106 798
Fragments arranged by size:
798 106 74
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 622 978
Size: 622 356
Bpu10I CC'TnA_GC
Cuts at: 0 733 978
Size: 733 245
BsaI GGTCTCn'nnnn_
Cuts at: 0 69 978
Size: 69 909
BsaWI w'CCGG_w
Cuts at: 0 255 338 978
Size: 255 83 640
Fragments arranged by size:
640 255 83
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 571 642 978
Size: 571 71 336
Fragments arranged by size:
571 336 71
BseYI C'CCAG_C
Cuts at: 0 796 978
Size: 796 182
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 917 978
Size: 917 61
BsiHKAI G_wGCw'C
Cuts at: 0 53 280 978
Size: 53 227 698
Fragments arranged by size:
698 227 53
Bsp1286I G_dGCh'C
Cuts at: 0 53 74 180 280 494 978
Size: 53 21 106 100 214 484
Fragments arranged by size:
484 214 106 100 53 21
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 244 978
Size: 244 734
BsrBI CCG'CTC
Cuts at: 0 459 978
Size: 459 519
BsrDI GCAATG_nn'
Cuts at: 0 812 978
Size: 812 166
BsrFI r'CCGG_y
Cuts at: 0 385 978
Size: 385 593
BstEII G'GTnAC_C
Cuts at: 0 354 978
Size: 354 624
BstXI CCAn_nnnn'nTGG
Cuts at: 0 705 978
Size: 705 273
BstYI r'GATC_y
Cuts at: 0 329 395 951 978
Size: 329 66 556 27
Fragments arranged by size:
556 329 66 27
BtgI C'CryG_G
Cuts at: 0 170 978
Size: 170 808
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 39 978
Size: 39 939
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 287 322 978
Size: 287 35 656
Fragments arranged by size:
656 287 35
EaeI y'GGCC_r
Cuts at: 0 167 377 842 978
Size: 167 210 465 136
Fragments arranged by size:
465 210 167 136
EarI CTCTTCn'nnn_
Cuts at: 0 724 778 956 978
Size: 724 54 178 22
Fragments arranged by size:
724 178 54 22
EcoICRI GAG'CTC
Cuts at: 0 278 978
Size: 278 700
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 622 709 978
Size: 622 87 269
Fragments arranged by size:
622 269 87
HaeII r_GCGC'y
Cuts at: 0 118 978
Size: 118 860
MfeI C'AATT_G
Cuts at: 0 299 978
Size: 299 679
MscI TGG'CCA
Cuts at: 0 379 978
Size: 379 599
MslI CAynn'nnrTG
Cuts at: 0 46 526 978
Size: 46 480 452
Fragments arranged by size:
480 452 46
MspA1I CmG'CkG
Cuts at: 0 737 923 978
Size: 737 186 55
Fragments arranged by size:
737 186 55
NaeI GCC'GGC
Cuts at: 0 387 978
Size: 387 591
NgoMIV G'CCGG_C
Cuts at: 0 385 978
Size: 385 593
PvuII CAG'CTG
Cuts at: 0 737 978
Size: 737 241
SacI G_AGCT'C
Cuts at: 0 280 978
Size: 280 698
ScaI AGT'ACT
Cuts at: 0 673 978
Size: 673 305
SmaI CCC'GGG
Cuts at: 0 75 978
Size: 75 903
SmlI C'TyrA_G
Cuts at: 0 898 978
Size: 898 80
StuI AGG'CCT
Cuts at: 0 969 978
Size: 969 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 163 286 552 978
Size: 163 123 266 426
Fragments arranged by size:
426 266 163 123
TatI w'GTAC_w
Cuts at: 0 533 671 978
Size: 533 138 307
Fragments arranged by size:
533 307 138
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 480 978
Size: 480 498
XmaI C'CCGG_G
Cuts at: 0 73 978
Size: 73 905
Enzymes that do cut:
AarI AccI AcuI AflII AlfI AlwNI AvaI BamHI
BanI BanII BbsI BbvCI BclI BglI Bme1580I BpmI
Bpu10I BsaI BsaWI BseRI BseYI BsgI BsiHKAI Bsp1286I
BspMI BsrBI BsrDI BsrFI BstEII BstXI BstYI BtgI
BtgZI CspCI EaeI EarI EcoICRI Eco57MI HaeII MfeI
MscI MslI MspA1I NaeI NgoMIV PvuII SacI ScaI
SmaI SmlI StuI TaqII TatI XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflIII AgeI AhdI AleI
AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BbeI BcgI BciVI BfrBI BglII BlpI BmgBI
BmrI BmtI BplI BpuEI BsaAI BsaBI BsaHI BsaXI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrGI BssHII
BssSI BstAPI BstBI BstZ17I Bsu36I BtsI ClaI DraI
DraIII DrdI EagI EciI EcoNI EcoO109I EcoRI EcoRV
FalI FseI FspI FspAI Hin4I HincII HindIII HpaI
KasI KpnI MluI MmeI NarI NcoI NdeI NheI
NotI NruI NsiI NspI PacI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI RsrII SacII SalI SanDI
SapI SbfI SexAI SfcI SfiI SfoI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI StyI SwaI TstI
Tth111I XbaI XhoI XmnI ZraI