Restriction map for
pF1KB8947
(Linear) (Six-base) MAPSORT of: KIBB8947.seq Check: 1282 from: 1 to: 942
KIBB8947.orf
With 182 enzymes: SgfI *
March 3, 2008 11:09 ..
AlwNI CAG_nnn'CTG
Cuts at: 0 801 942
Size: 801 141
BanI G'GyrC_C
Cuts at: 0 811 942
Size: 811 131
BanII G_rGCy'C
Cuts at: 0 406 449 942
Size: 406 43 493
Fragments arranged by size:
493 406 43
BbsI GAAGACnn'nnnn_
Cuts at: 0 288 921 942
Size: 288 633 21
Fragments arranged by size:
633 288 21
BglI GCCn_nnn'nGGC
Cuts at: 0 694 806 942
Size: 694 112 136
Fragments arranged by size:
694 136 112
BlpI GC'TnA_GC
Cuts at: 0 511 942
Size: 511 431
BmgBI CAC'GTC
Cuts at: 0 378 942
Size: 378 564
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 322 373 856 877 942
Size: 196 126 51 483 21 65
Fragments arranged by size:
483 196 126 65 51 21
Bpu10I CC'TnA_GC
Cuts at: 0 223 469 942
Size: 223 246 473
Fragments arranged by size:
473 246 223
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 865 919 942
Size: 865 54 23
Fragments arranged by size:
865 54 23
BsaI GGTCTCn'nnnn_
Cuts at: 0 349 942
Size: 349 593
BsaAI yAC'GTr
Cuts at: 0 173 441 942
Size: 173 268 501
Fragments arranged by size:
501 268 173
BsaHI Gr'CG_yC
Cuts at: 0 781 942
Size: 781 161
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 143 173 322 352 942
Size: 143 30 149 30 590
Fragments arranged by size:
590 149 143 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 167 589 874 942
Size: 167 422 285 68
Fragments arranged by size:
422 285 167 68
BseYI C'CCAG_C
Cuts at: 0 276 463 900 942
Size: 276 187 437 42
Fragments arranged by size:
437 276 187 42
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 496 942
Size: 496 446
Bsp1286I G_dGCh'C
Cuts at: 0 406 449 942
Size: 406 43 493
Fragments arranged by size:
493 406 43
BspHI T'CATG_A
Cuts at: 0 287 942
Size: 287 655
BssHII G'CGCG_C
Cuts at: 0 419 489 942
Size: 419 70 453
Fragments arranged by size:
453 419 70
BssSI C'ACGA_G
Cuts at: 0 619 942
Size: 619 323
BstEII G'GTnAC_C
Cuts at: 0 699 942
Size: 699 243
BstXI CCAn_nnnn'nTGG
Cuts at: 0 855 942
Size: 855 87
BstYI r'GATC_y
Cuts at: 0 125 839 942
Size: 125 714 103
Fragments arranged by size:
714 125 103
BtgI C'CryG_G
Cuts at: 0 450 456 635 942
Size: 450 6 179 307
Fragments arranged by size:
450 307 179 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 97 942
Size: 97 845
EaeI y'GGCC_r
Cuts at: 0 17 167 231 257 453 611 632 686
Size: 17 150 64 26 196 158 21 54
Cuts at: 686 845 917 942
Size: 159 72 25
Fragments arranged by size:
196 159 158 150 72 64 54 26
25 21 17
EarI CTCTTCn'nnn_
Cuts at: 0 826 859 895 942
Size: 826 33 36 47
Fragments arranged by size:
826 47 36 33
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 322 373 856 877 942
Size: 196 126 51 483 21 65
Fragments arranged by size:
483 196 126 65 51 21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 571 847 942
Size: 571 276 95
Fragments arranged by size:
571 276 95
EcoO109I rG'GnC_Cy
Cuts at: 0 219 480 552 798 885 942
Size: 219 261 72 246 87 57
Fragments arranged by size:
261 246 219 87 72 57
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 357 906 942
Size: 357 549 36
Fragments arranged by size:
549 357 36
HaeII r_GCGC'y
Cuts at: 0 713 758 942
Size: 713 45 184
Fragments arranged by size:
713 184 45
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 117 149 322 354 858 890 942
Size: 117 32 173 32 504 32 52
Fragments arranged by size:
504 173 117 52 32 32 32
MscI TGG'CCA
Cuts at: 0 19 169 847 942
Size: 19 150 678 95
Fragments arranged by size:
678 150 95 19
MslI CAynn'nnrTG
Cuts at: 0 652 942
Size: 652 290
MspA1I CmG'CkG
Cuts at: 0 196 255 309 942
Size: 196 59 54 633
Fragments arranged by size:
633 196 59 54
PasI CC'CwG_GG
Cuts at: 0 561 642 723 759 942
Size: 561 81 81 36 183
Fragments arranged by size:
561 183 81 81 36
PfoI T'CCnGG_A
Cuts at: 0 299 833 942
Size: 299 534 109
Fragments arranged by size:
534 299 109
PmlI CAC'GTG
Cuts at: 0 173 942
Size: 173 769
PpuMI rG'GwC_Cy
Cuts at: 0 219 885 942
Size: 219 666 57
Fragments arranged by size:
666 219 57
PstI C_TGCA'G
Cuts at: 0 572 797 942
Size: 572 225 145
Fragments arranged by size:
572 225 145
PvuII CAG'CTG
Cuts at: 0 309 942
Size: 309 633
SbfI CC_TGCA'GG
Cuts at: 0 572 942
Size: 572 370
SfcI C'TryA_G
Cuts at: 0 568 793 942
Size: 568 225 149
Fragments arranged by size:
568 225 149
SmlI C'TyrA_G
Cuts at: 0 880 934 942
Size: 880 54 8
Fragments arranged by size:
880 54 8
StyI C'CwwG_G
Cuts at: 0 848 942
Size: 848 94
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 531 942
Size: 531 411
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 143 175 362 394 942
Size: 143 32 187 32 548
Fragments arranged by size:
548 187 143 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 273 720 942
Size: 273 447 222
Fragments arranged by size:
447 273 222
Enzymes that do cut:
AlwNI BanI BanII BbsI BglI BlpI BmgBI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI
BsgI Bsp1286I BspHI BssHII BssSI BstEII BstXI BstYI
BtgI BtgZI EaeI EarI Eco57MI EcoNI EcoO109I FalI
HaeII Hin4I MscI MslI MspA1I PasI PfoI PmlI
PpuMI PstI PvuII SbfI SfcI SmlI StyI TaqII
TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI ApaI ApaLI
ApoI AscI AseI AsiSI AvaI AvrII BaeI BamHI
BbeI BbvCI BcgI BciVI BclI BfrBI BglII Bme1580I
BmrI BmtI BplI BsaBI BsaWI BsiEI BsiHKAI BsiWI
BsmI BsmBI BspEI BspMI BsrBI BsrDI BsrFI BsrGI
BstAPI BstBI BstZ17I Bsu36I BtsI ClaI CspCI DraI
DraIII DrdI EagI EciI EcoICRI EcoRI EcoRV FseI
FspI FspAI HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PciI PflMI PmeI
PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SapI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI TatI Tth111I XbaI XhoI XmaI
XmnI ZraI