Restriction map for
pF1KB8946
(Linear) (Six-base) MAPSORT of: KIBB8946.seq Check: 9709 from: 1 to: 939
KIBB8946.orf
With 182 enzymes: SgfI *
March 3, 2008 11:09 ..
AccI GT'mk_AC
Cuts at: 0 341 791 939
Size: 341 450 148
Fragments arranged by size:
450 341 148
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 168 768 939
Size: 168 600 171
Fragments arranged by size:
600 171 168
AfeI AGC'GCT
Cuts at: 0 733 939
Size: 733 206
AflIII A'CryG_T
Cuts at: 0 35 939
Size: 35 904
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 760 792 939
Size: 760 32 147
Fragments arranged by size:
760 147 32
AlwNI CAG_nnn'CTG
Cuts at: 0 275 939
Size: 275 664
ApaI G_GGCC'C
Cuts at: 0 506 939
Size: 506 433
ApaLI G'TGCA_C
Cuts at: 0 265 939
Size: 265 674
AscI GG'CGCG_CC
Cuts at: 0 554 939
Size: 554 385
AvaI C'yCGr_G
Cuts at: 0 66 478 646 664 939
Size: 66 412 168 18 275
Fragments arranged by size:
412 275 168 66 18
BanI G'GyrC_C
Cuts at: 0 218 285 445 641 939
Size: 218 67 160 196 298
Fragments arranged by size:
298 218 196 160 67
BanII G_rGCy'C
Cuts at: 0 206 263 331 506 939
Size: 206 57 68 175 433
Fragments arranged by size:
433 206 175 68 57
BbeI G_GCGC'C
Cuts at: 0 289 645 939
Size: 289 356 294
Fragments arranged by size:
356 294 289
BbsI GAAGACnn'nnnn_
Cuts at: 0 107 665 939
Size: 107 558 274
Fragments arranged by size:
558 274 107
Bme1580I G_kGCm'C
Cuts at: 0 269 506 939
Size: 269 237 433
Fragments arranged by size:
433 269 237
BmgBI CAC'GTC
Cuts at: 0 315 939
Size: 315 624
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 66 690 939
Size: 66 624 249
Fragments arranged by size:
624 249 66
BsaI GGTCTCn'nnnn_
Cuts at: 0 896 939
Size: 896 43
BsaHI Gr'CG_yC
Cuts at: 0 286 401 642 939
Size: 286 115 241 297
Fragments arranged by size:
297 286 241 115
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 124 154 232 262 448 478 939
Size: 124 30 78 30 186 30 461
Fragments arranged by size:
461 186 124 78 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 18 132 271 456 637 925 939
Size: 18 114 139 185 181 288 14
Fragments arranged by size:
288 185 181 139 114 18 14
BseYI C'CCAG_C
Cuts at: 0 289 939
Size: 289 650
BsiEI CG_ry'CG
Cuts at: 0 299 939
Size: 299 640
BsiHKAI G_wGCw'C
Cuts at: 0 263 269 331 939
Size: 263 6 62 608
Fragments arranged by size:
608 263 62 6
BsmBI CGTCTCn'nnnn_
Cuts at: 0 69 598 939
Size: 69 529 341
Fragments arranged by size:
529 341 69
Bsp1286I G_dGCh'C
Cuts at: 0 206 263 269 331 506 939
Size: 206 57 6 62 175 433
Fragments arranged by size:
433 206 175 62 57 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 158 939
Size: 158 781
BsrBI CCG'CTC
Cuts at: 0 633 939
Size: 633 306
BsrFI r'CCGG_y
Cuts at: 0 298 558 697 939
Size: 298 260 139 242
Fragments arranged by size:
298 260 242 139
BssHII G'CGCG_C
Cuts at: 0 554 939
Size: 554 385
BssSI C'ACGA_G
Cuts at: 0 262 939
Size: 262 677
BstYI r'GATC_y
Cuts at: 0 755 939
Size: 755 184
BtgI C'CryG_G
Cuts at: 0 225 251 386 515 527 939
Size: 225 26 135 129 12 412
Fragments arranged by size:
412 225 135 129 26 12
BtsI GCAGTG_nn'
Cuts at: 0 240 939
Size: 240 699
EaeI y'GGCC_r
Cuts at: 0 296 320 332 512 678 711 939
Size: 296 24 12 180 166 33 228
Fragments arranged by size:
296 228 180 166 33 24 12
EagI C'GGCC_G
Cuts at: 0 296 939
Size: 296 643
EcoICRI GAG'CTC
Cuts at: 0 261 329 939
Size: 261 68 610
Fragments arranged by size:
610 261 68
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 66 168 690 768 939
Size: 66 102 522 78 171
Fragments arranged by size:
522 171 102 78 66
EcoNI CCTnn'n_nnAGG
Cuts at: 0 154 409 939
Size: 154 255 530
Fragments arranged by size:
530 255 154
EcoO109I rG'GnC_Cy
Cuts at: 0 502 503 939
Size: 502 1 436
Fragments arranged by size:
502 436 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 71 939
Size: 71 868
HaeII r_GCGC'y
Cuts at: 0 289 494 645 735 813 939
Size: 289 205 151 90 78 126
Fragments arranged by size:
289 205 151 126 90 78
KasI G'GCGC_C
Cuts at: 0 285 641 939
Size: 285 356 298
Fragments arranged by size:
356 298 285
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 170 186 494 939
Size: 170 16 308 445
Fragments arranged by size:
445 308 170 16
MscI TGG'CCA
Cuts at: 0 322 713 939
Size: 322 391 226
Fragments arranged by size:
391 322 226
MspA1I CmG'CkG
Cuts at: 0 227 253 529 581 939
Size: 227 26 276 52 358
Fragments arranged by size:
358 276 227 52 26
NaeI GCC'GGC
Cuts at: 0 300 560 699 939
Size: 300 260 139 240
Fragments arranged by size:
300 260 240 139
NarI GG'CG_CC
Cuts at: 0 286 642 939
Size: 286 356 297
Fragments arranged by size:
356 297 286
NgoMIV G'CCGG_C
Cuts at: 0 298 558 697 939
Size: 298 260 139 242
Fragments arranged by size:
298 260 242 139
NspI r_CATG'y
Cuts at: 0 39 831 939
Size: 39 792 108
Fragments arranged by size:
792 108 39
PciI A'CATG_T
Cuts at: 0 35 939
Size: 35 904
PflMI CCAn_nnn'nTGG
Cuts at: 0 215 939
Size: 215 724
PspOMI G'GGCC_C
Cuts at: 0 502 939
Size: 502 437
PstI C_TGCA'G
Cuts at: 0 284 939
Size: 284 655
SacI G_AGCT'C
Cuts at: 0 263 331 939
Size: 263 68 608
Fragments arranged by size:
608 263 68
SacII CC_GC'GG
Cuts at: 0 228 254 530 939
Size: 228 26 276 409
Fragments arranged by size:
409 276 228 26
SbfI CC_TGCA'GG
Cuts at: 0 284 939
Size: 284 655
ScaI AGT'ACT
Cuts at: 0 916 939
Size: 916 23
SfcI C'TryA_G
Cuts at: 0 280 378 939
Size: 280 98 561
Fragments arranged by size:
561 280 98
SfoI GGC'GCC
Cuts at: 0 287 643 939
Size: 287 356 296
Fragments arranged by size:
356 296 287
SmaI CCC'GGG
Cuts at: 0 480 939
Size: 480 459
SphI G_CATG'C
Cuts at: 0 831 939
Size: 831 108
TatI w'GTAC_w
Cuts at: 0 52 914 939
Size: 52 862 25
Fragments arranged by size:
862 52 25
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 232 264 939
Size: 232 32 675
Fragments arranged by size:
675 232 32
Tth111I GACn'n_nGTC
Cuts at: 0 111 939
Size: 111 828
XmaI C'CCGG_G
Cuts at: 0 478 939
Size: 478 461
Enzymes that do cut:
AccI AcuI AfeI AflIII AloI AlwNI ApaI ApaLI
AscI AvaI BanI BanII BbeI BbsI Bme1580I BmgBI
BpmI BsaI BsaHI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmBI Bsp1286I BspMI BsrBI BsrFI BssHII BssSI BstYI
BtgI BtsI EaeI EagI EcoICRI Eco57MI EcoNI EcoO109I
FalI HaeII KasI MmeI MscI MspA1I NaeI NarI
NgoMIV NspI PciI PflMI PspOMI PstI SacI SacII
SbfI ScaI SfcI SfoI SmaI SphI TatI TstI
Tth111I XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AgeI AhdI AleI
AlfI ApoI AseI AsiSI AvrII BaeI BamHI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI BmrI
BmtI BplI Bpu10I BpuEI BsaAI BsaBI BsaWI BsgI
BsiWI BsmI BspEI BspHI BsrDI BsrGI BstAPI BstBI
BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DraIII DrdI EarI EciI EcoRI EcoRV FseI FspI
FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI
MslI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspXI PsrI PvuI PvuII RsrII SalI SanDI SapI
SexAI SfiI SgfI SgrAI SmlI SnaBI SpeI SrfI
SspI StuI StyI SwaI TaqII XbaI XcmI XhoI
XmnI ZraI