Restriction map for
pF1KB8942
(Linear) (Six-base) MAPSORT of: KIBB8942.seq Check: 7070 from: 1 to: 903
KIBB8942.orf
With 182 enzymes: SgfI *
March 3, 2008 11:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 417 690 781 903
Size: 417 273 91 122
Fragments arranged by size:
417 273 122 91
Acc65I G'GTAC_C
Cuts at: 0 464 903
Size: 464 439
AlwNI CAG_nnn'CTG
Cuts at: 0 129 432 899 903
Size: 129 303 467 4
Fragments arranged by size:
467 303 129 4
ApaI G_GGCC'C
Cuts at: 0 667 674 903
Size: 667 7 229
Fragments arranged by size:
667 229 7
AscI GG'CGCG_CC
Cuts at: 0 785 903
Size: 785 118
AvaI C'yCGr_G
Cuts at: 0 478 667 792 903
Size: 478 189 125 111
Fragments arranged by size:
478 189 125 111
BanI G'GyrC_C
Cuts at: 0 318 464 769 903
Size: 318 146 305 134
Fragments arranged by size:
318 305 146 134
BanII G_rGCy'C
Cuts at: 0 13 112 667 674 903
Size: 13 99 555 7 229
Fragments arranged by size:
555 229 99 13 7
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 695 729 903
Size: 695 34 174
Fragments arranged by size:
695 174 34
BglII A'GATC_T
Cuts at: 0 530 903
Size: 530 373
BlpI GC'TnA_GC
Cuts at: 0 7 903
Size: 7 896
Bme1580I G_kGCm'C
Cuts at: 0 632 667 674 903
Size: 632 35 7 229
Fragments arranged by size:
632 229 35 7
BmgBI CAC'GTC
Cuts at: 0 515 903
Size: 515 388
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 460 903
Size: 76 384 443
Fragments arranged by size:
443 384 76
BseYI C'CCAG_C
Cuts at: 0 691 903
Size: 691 212
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 725 903
Size: 725 178
BsiHKAI G_wGCw'C
Cuts at: 0 413 491 750 903
Size: 413 78 259 153
Fragments arranged by size:
413 259 153 78
BsmI GAATG_Cn'
Cuts at: 0 855 903
Size: 855 48
Bsp1286I G_dGCh'C
Cuts at: 0 13 112 413 491 632 667 674 750
Size: 13 99 301 78 141 35 7 76
Cuts at: 750 903
Size: 153
Fragments arranged by size:
301 153 141 99 78 76 35 13
7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 417 513 690 781 903
Size: 417 96 177 91 122
Fragments arranged by size:
417 177 122 96 91
BsrBI CCG'CTC
Cuts at: 0 66 368 444 903
Size: 66 302 76 459
Fragments arranged by size:
459 302 76 66
BssHII G'CGCG_C
Cuts at: 0 500 785 903
Size: 500 285 118
Fragments arranged by size:
500 285 118
BstAPI GCAn_nnn'nTGC
Cuts at: 0 656 903
Size: 656 247
BstYI r'GATC_y
Cuts at: 0 530 903
Size: 530 373
BtgI C'CryG_G
Cuts at: 0 77 94 903
Size: 77 17 809
Fragments arranged by size:
809 77 17
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 659 903
Size: 659 244
BtsI GCAGTG_nn'
Cuts at: 0 716 903
Size: 716 187
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 156 191 903
Size: 156 35 712
Fragments arranged by size:
712 156 35
EaeI y'GGCC_r
Cuts at: 0 363 494 597 818 843 903
Size: 363 131 103 221 25 60
Fragments arranged by size:
363 221 131 103 60 25
EarI CTCTTCn'nnn_
Cuts at: 0 100 903
Size: 100 803
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 460 903
Size: 76 384 443
Fragments arranged by size:
443 384 76
EcoO109I rG'GnC_Cy
Cuts at: 0 223 664 796 896 903
Size: 223 441 132 100 7
Fragments arranged by size:
441 223 132 100 7
HaeII r_GCGC'y
Cuts at: 0 450 478 573 903
Size: 450 28 95 330
Fragments arranged by size:
450 330 95 28
KpnI G_GTAC'C
Cuts at: 0 468 903
Size: 468 435
MscI TGG'CCA
Cuts at: 0 496 599 820 845 903
Size: 496 103 221 25 58
Fragments arranged by size:
496 221 103 58 25
MspA1I CmG'CkG
Cuts at: 0 79 96 344 462 743 903
Size: 79 17 248 118 281 160
Fragments arranged by size:
281 248 160 118 79 17
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 277 309 903
Size: 277 32 594
Fragments arranged by size:
594 277 32
PpuMI rG'GwC_Cy
Cuts at: 0 223 796 903
Size: 223 573 107
Fragments arranged by size:
573 223 107
PspOMI G'GGCC_C
Cuts at: 0 663 670 903
Size: 663 7 233
Fragments arranged by size:
663 233 7
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 535 567 903
Size: 535 32 336
Fragments arranged by size:
535 336 32
PstI C_TGCA'G
Cuts at: 0 509 887 903
Size: 509 378 16
Fragments arranged by size:
509 378 16
SacII CC_GC'GG
Cuts at: 0 80 97 903
Size: 80 17 806
Fragments arranged by size:
806 80 17
SanDI GG'GwC_CC
Cuts at: 0 223 903
Size: 223 680
SapI GCTCTTCn'nnn_
Cuts at: 0 100 903
Size: 100 803
SfcI C'TryA_G
Cuts at: 0 505 699 738 883 903
Size: 505 194 39 145 20
Fragments arranged by size:
505 194 145 39 20
SmaI CCC'GGG
Cuts at: 0 669 903
Size: 669 234
StyI C'CwwG_G
Cuts at: 0 380 903
Size: 380 523
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 24 903
Size: 24 879
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 671 703 903
Size: 671 32 200
Fragments arranged by size:
671 200 32
XmaI C'CCGG_G
Cuts at: 0 667 903
Size: 667 236
XmnI GAAnn'nnTTC
Cuts at: 0 119 903
Size: 119 784
Enzymes that do cut:
AarI Acc65I AlwNI ApaI AscI AvaI BanI BanII
BcgI BglII BlpI Bme1580I BmgBI BpmI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspMI BsrBI BssHII BstAPI BstYI
BtgI BtgZI BtsI CspCI EaeI EarI Eco57MI EcoO109I
HaeII KpnI MscI MspA1I PpiI PpuMI PspOMI PsrI
PstI SacII SanDI SapI SfcI SmaI StyI TaqII
TstI XmaI XmnI
Enzymes that do not cut:
AatII AccI AclI AcuI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI ApoI AseI AsiSI
AvrII BaeI BamHI BbeI BbsI BbvCI BciVI BclI
BfrBI BglI BmrI BmtI BplI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BsiEI BsiWI
BsmBI BspEI BspHI BsrDI BsrFI BsrGI BssSI BstBI
BstEII BstXI BstZ17I Bsu36I ClaI DraI DraIII DrdI
EagI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI Hin4I HincII HindIII HpaI KasI MfeI
MluI MmeI MslI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PflMI PfoI PmeI PmlI PshAI PsiI PspXI PvuI
PvuII RsrII SacI SalI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI TatI Tth111I XbaI XcmI XhoI
ZraI