Restriction map for
pF1KB8938
(Linear) (Six-base) MAPSORT of: KIBB8938.seq Check: 3315 from: 1 to: 870
KIBB8938.orf
With 182 enzymes: SgfI *
March 3, 2008 11:09 ..
AccI GT'mk_AC
Cuts at: 0 588 870
Size: 588 282
AlwNI CAG_nnn'CTG
Cuts at: 0 583 870
Size: 583 287
ApoI r'AATT_y
Cuts at: 0 643 778 870
Size: 643 135 92
Fragments arranged by size:
643 135 92
AvaI C'yCGr_G
Cuts at: 0 112 870
Size: 112 758
BanII G_rGCy'C
Cuts at: 0 398 538 870
Size: 398 140 332
Fragments arranged by size:
398 332 140
BbsI GAAGACnn'nnnn_
Cuts at: 0 606 851 870
Size: 606 245 19
Fragments arranged by size:
606 245 19
BciVI GTATCCnnnnn_n'
Cuts at: 0 406 870
Size: 406 464
BglI GCCn_nnn'nGGC
Cuts at: 0 316 870
Size: 316 554
BmrI ACTGGGnnnn_n'
Cuts at: 0 251 870
Size: 251 619
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 557 870
Size: 557 313
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 859 870
Size: 859 11
BsaI GGTCTCn'nnnn_
Cuts at: 0 561 870
Size: 561 309
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 320 323 870
Size: 320 3 547
Fragments arranged by size:
547 320 3
BseYI C'CCAG_C
Cuts at: 0 449 870
Size: 449 421
BsiEI CG_ry'CG
Cuts at: 0 126 305 870
Size: 126 179 565
Fragments arranged by size:
565 179 126
BsiHKAI G_wGCw'C
Cuts at: 0 538 870
Size: 538 332
BsmBI CGTCTCn'nnnn_
Cuts at: 0 545 870
Size: 545 325
Bsp1286I G_dGCh'C
Cuts at: 0 398 538 870
Size: 398 140 332
Fragments arranged by size:
398 332 140
BsrFI r'CCGG_y
Cuts at: 0 175 870
Size: 175 695
BtgI C'CryG_G
Cuts at: 0 559 870
Size: 559 311
EaeI y'GGCC_r
Cuts at: 0 123 302 870
Size: 123 179 568
Fragments arranged by size:
568 179 123
EagI C'GGCC_G
Cuts at: 0 123 302 870
Size: 123 179 568
Fragments arranged by size:
568 179 123
EarI CTCTTCn'nnn_
Cuts at: 0 588 870
Size: 588 282
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 335 341 870
Size: 335 6 529
Fragments arranged by size:
529 335 6
EcoICRI GAG'CTC
Cuts at: 0 536 870
Size: 536 334
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 859 870
Size: 859 11
EcoO109I rG'GnC_Cy
Cuts at: 0 513 870
Size: 513 357
HaeII r_GCGC'y
Cuts at: 0 173 870
Size: 173 697
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 369 401 472 504 870
Size: 369 32 71 32 366
Fragments arranged by size:
369 366 71 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 614 870
Size: 614 256
MspA1I CmG'CkG
Cuts at: 0 24 307 870
Size: 24 283 563
Fragments arranged by size:
563 283 24
NaeI GCC'GGC
Cuts at: 0 177 870
Size: 177 693
NgoMIV G'CCGG_C
Cuts at: 0 175 870
Size: 175 695
PflMI CCAn_nnn'nTGG
Cuts at: 0 837 870
Size: 837 33
PfoI T'CCnGG_A
Cuts at: 0 235 870
Size: 235 635
PpuMI rG'GwC_Cy
Cuts at: 0 513 870
Size: 513 357
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 10 42 870
Size: 10 32 828
Fragments arranged by size:
828 32 10
PstI C_TGCA'G
Cuts at: 0 155 870
Size: 155 715
SacI G_AGCT'C
Cuts at: 0 538 870
Size: 538 332
ScaI AGT'ACT
Cuts at: 0 277 870
Size: 277 593
SfcI C'TryA_G
Cuts at: 0 151 609 870
Size: 151 458 261
Fragments arranged by size:
458 261 151
SspI AAT'ATT
Cuts at: 0 504 870
Size: 504 366
StuI AGG'CCT
Cuts at: 0 860 870
Size: 860 10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 233 870
Size: 233 637
TatI w'GTAC_w
Cuts at: 0 26 35 275 870
Size: 26 9 240 595
Fragments arranged by size:
595 240 26 9
XbaI T'CTAG_A
Cuts at: 0 627 870
Size: 627 243
Enzymes that do cut:
AccI AlwNI ApoI AvaI BanII BbsI BciVI BglI
BmrI BplI BpmI BsaI BseRI BseYI BsiEI BsiHKAI
BsmBI Bsp1286I BsrFI BtgI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I HaeII Hin4I MmeI MspA1I NaeI
NgoMIV PflMI PfoI PpuMI PsrI PstI SacI ScaI
SfcI SspI StuI TaqII TatI XbaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BanI BbeI BbvCI
BcgI BclI BfrBI BglII BlpI Bme1580I BmgBI BmtI
Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI BsgI
BsiWI BsmI BspEI BspHI BspMI BsrBI BsrDI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI KasI KpnI MfeI MluI MscI MslI
NarI NcoI NdeI NheI NotI NruI NsiI NspI
PacI PasI PciI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PvuI PvuII RsrII SacII SalI SanDI
SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI StyI SwaI TstI
Tth111I XcmI XhoI XmaI XmnI ZraI