(Linear) (Six-base) MAPSORT of: KIBB8935.seq  Check: 4977  from: 1  to: 852



KIBB8935.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:09  ..

AflIII A'CryG_T

Cuts at:      0     733     852 
   Size:        733     119
 
ApoI r'AATT_y

Cuts at:      0     561     852 
   Size:        561     291
 
AvaI C'yCGr_G

Cuts at:      0     314     341     852 
   Size:        314      27     511

  Fragments arranged by size: 

                 511     314      27
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     311     344     852 
   Size:        311      33     508

  Fragments arranged by size: 

                 508     311      33
 
BanI G'GyrC_C

Cuts at:      0     345     852 
   Size:        345     507
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     218     252     852 
   Size:        218      34     600

  Fragments arranged by size: 

                 600     218      34
 
Bme1580I G_kGCm'C

Cuts at:      0     348     852 
   Size:        348     504
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     388     852 
   Size:        388     464
 
Bsp1286I G_dGCh'C

Cuts at:      0     348     852 
   Size:        348     504
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     234     852 
   Size:        234     618
 
BsrDI GCAATG_nn'

Cuts at:      0     288     422     852 
   Size:        288     134     430

  Fragments arranged by size: 

                 430     288     134
 
BstBI TT'CG_AA

Cuts at:      0     405     852 
   Size:        405     447
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      33     852 
   Size:         33     819
 
BstYI r'GATC_y

Cuts at:      0     454     852 
   Size:        454     398
 
Bsu36I CC'TnA_GG

Cuts at:      0     251     852 
   Size:        251     601
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     221     852 
   Size:        221     631
 
ClaI AT'CG_AT

Cuts at:      0     837     852 
   Size:        837      15
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     690     725     852 
   Size:        690      35     127

  Fragments arranged by size: 

                 690     127      35
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      13     852 
   Size:         13     839
 
EaeI y'GGCC_r

Cuts at:      0     698     852 
   Size:        698     154
 
EarI CTCTTCn'nnn_

Cuts at:      0     407     852 
   Size:        407     445
 
EcoO109I rG'GnC_Cy

Cuts at:      0     713     852 
   Size:        713     139
 
EcoRV GAT'ATC

Cuts at:      0     533     852 
   Size:        533     319
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     172     204     297     329     522     554     852 
   Size:        172      32      93      32     193      32     298

  Fragments arranged by size: 

                 298     193     172      93      32      32      32
 
MfeI C'AATT_G

Cuts at:      0     428     852 
   Size:        428     424
 
MscI TGG'CCA

Cuts at:      0     700     852 
   Size:        700     152
 
MspA1I CmG'CkG

Cuts at:      0     278     852 
   Size:        278     574
 
NspI r_CATG'y

Cuts at:      0     737     852 
   Size:        737     115
 
PciI A'CATG_T

Cuts at:      0     733     852 
   Size:        733     119
 
PfoI T'CCnGG_A

Cuts at:      0     163     852 
   Size:        163     689
 
PpuMI rG'GwC_Cy

Cuts at:      0     713     852 
   Size:        713     139
 
PstI C_TGCA'G

Cuts at:      0      74     399     852 
   Size:         74     325     453

  Fragments arranged by size: 

                 453     325      74
 
SanDI GG'GwC_CC

Cuts at:      0     713     852 
   Size:        713     139
 
SapI GCTCTTCn'nnn_

Cuts at:      0     407     852 
   Size:        407     445
 
ScaI AGT'ACT

Cuts at:      0     270     852 
   Size:        270     582
 
SfcI C'TryA_G

Cuts at:      0      70     395     852 
   Size:         70     325     457

  Fragments arranged by size: 

                 457     325      70
 
SmlI C'TyrA_G

Cuts at:      0     314     852 
   Size:        314     538
 
SspI AAT'ATT

Cuts at:      0      48     591     852 
   Size:         48     543     261

  Fragments arranged by size: 

                 543     261      48
 
TatI w'GTAC_w

Cuts at:      0     118     268     852 
   Size:        118     150     584

  Fragments arranged by size: 

                 584     150     118
 
XhoI C'TCGA_G

Cuts at:      0     314     852 
   Size:        314     538
 

 Enzymes that do cut:

   AflIII     ApoI     AvaI     BaeI     BanI     BcgI Bme1580I     BsgI
 Bsp1286I    BspMI    BsrDI    BstBI    BstXI    BstYI   Bsu36I    BtgZI
     ClaI    CspCI     DrdI     EaeI     EarI EcoO109I    EcoRV    Hin4I
     MfeI     MscI   MspA1I     NspI     PciI     PfoI    PpuMI     PstI
    SanDI     SapI     ScaI     SfcI     SmlI     SspI     TatI     XhoI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI    AvrII    BamHI    BanII     BbeI     BbsI
    BbvCI    BciVI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI
     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI   BstEII  BstZ17I     BtgI     BtsI     DraI
   DraIII     EagI     EciI  EcoICRI  Eco57MI    EcoNI    EcoRI     FalI
     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI
     KpnI     MluI     MmeI     MslI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI
     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    PvuII    RsrII     SacI    SacII     SalI     SbfI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     StuI     StyI     SwaI    TaqII     TstI  Tth111I     XbaI
     XcmI     XmaI     XmnI     ZraI