Restriction map for
pF1KB8934
(Linear) (Six-base) MAPSORT of: KIBB8934.seq Check: 5441 from: 1 to: 852
KIBB8934.orf
With 182 enzymes: SgfI *
March 3, 2008 11:09 ..
AatII G_ACGT'C
Cuts at: 0 220 852
Size: 220 632
AfeI AGC'GCT
Cuts at: 0 843 852
Size: 843 9
AflIII A'CryG_T
Cuts at: 0 434 852
Size: 434 418
AlwNI CAG_nnn'CTG
Cuts at: 0 453 852
Size: 453 399
BanI G'GyrC_C
Cuts at: 0 114 204 852
Size: 114 90 648
Fragments arranged by size:
648 114 90
BanII G_rGCy'C
Cuts at: 0 8 286 519 852
Size: 8 278 233 333
Fragments arranged by size:
333 278 233 8
BbeI G_GCGC'C
Cuts at: 0 118 852
Size: 118 734
BglI GCCn_nnn'nGGC
Cuts at: 0 316 852
Size: 316 536
BglII A'GATC_T
Cuts at: 0 743 852
Size: 743 109
Bme1580I G_kGCm'C
Cuts at: 0 209 303 852
Size: 209 94 549
Fragments arranged by size:
549 209 94
BmgBI CAC'GTC
Cuts at: 0 435 852
Size: 435 417
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 485 588 712 852
Size: 485 103 124 140
Fragments arranged by size:
485 140 124 103
BsaBI GATnn'nnATC
Cuts at: 0 781 852
Size: 781 71
BsaHI Gr'CG_yC
Cuts at: 0 115 217 626 852
Size: 115 102 409 226
Fragments arranged by size:
409 226 115 102
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 685 715 852
Size: 685 30 137
Fragments arranged by size:
685 137 30
BseYI C'CCAG_C
Cuts at: 0 54 108 222 361 852
Size: 54 54 114 139 491
Fragments arranged by size:
491 139 114 54 54
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 466 852
Size: 466 386
BsiEI CG_ry'CG
Cuts at: 0 347 648 852
Size: 347 301 204
Fragments arranged by size:
347 301 204
BsiHKAI G_wGCw'C
Cuts at: 0 286 462 852
Size: 286 176 390
Fragments arranged by size:
390 286 176
BsmI GAATG_Cn'
Cuts at: 0 659 852
Size: 659 193
Bsp1286I G_dGCh'C
Cuts at: 0 8 209 286 303 462 519 852
Size: 8 201 77 17 159 57 333
Fragments arranged by size:
333 201 159 77 57 17 8
BsrBI CCG'CTC
Cuts at: 0 61 696 852
Size: 61 635 156
Fragments arranged by size:
635 156 61
BsrDI GCAATG_nn'
Cuts at: 0 190 852
Size: 190 662
BstAPI GCAn_nnn'nTGC
Cuts at: 0 595 852
Size: 595 257
BstXI CCAn_nnnn'nTGG
Cuts at: 0 429 852
Size: 429 423
BstYI r'GATC_y
Cuts at: 0 153 743 852
Size: 153 590 109
Fragments arranged by size:
590 153 109
BtgI C'CryG_G
Cuts at: 0 329 341 852
Size: 329 12 511
Fragments arranged by size:
511 329 12
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 360 395 852
Size: 360 35 457
Fragments arranged by size:
457 360 35
EaeI y'GGCC_r
Cuts at: 0 317 344 419 645 852
Size: 317 27 75 226 207
Fragments arranged by size:
317 226 207 75 27
EagI C'GGCC_G
Cuts at: 0 344 645 852
Size: 344 301 207
Fragments arranged by size:
344 301 207
EarI CTCTTCn'nnn_
Cuts at: 0 347 852
Size: 347 505
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 87 184 852
Size: 87 97 668
Fragments arranged by size:
668 97 87
EcoICRI GAG'CTC
Cuts at: 0 284 852
Size: 284 568
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 485 588 712 852
Size: 485 103 124 140
Fragments arranged by size:
485 140 124 103
EcoO109I rG'GnC_Cy
Cuts at: 0 574 852
Size: 574 278
EcoRV GAT'ATC
Cuts at: 0 42 397 852
Size: 42 355 455
Fragments arranged by size:
455 355 42
HaeII r_GCGC'y
Cuts at: 0 118 774 845 852
Size: 118 656 71 7
Fragments arranged by size:
656 118 71 7
HincII GTy'rAC
Cuts at: 0 138 852
Size: 138 714
KasI G'GCGC_C
Cuts at: 0 114 852
Size: 114 738
MscI TGG'CCA
Cuts at: 0 421 852
Size: 421 431
MspA1I CmG'CkG
Cuts at: 0 112 331 608 852
Size: 112 219 277 244
Fragments arranged by size:
277 244 219 112
NarI GG'CG_CC
Cuts at: 0 115 852
Size: 115 737
NotI GC'GGCC_GC
Cuts at: 0 645 852
Size: 645 207
PasI CC'CwG_GG
Cuts at: 0 199 210 852
Size: 199 11 642
Fragments arranged by size:
642 199 11
PflMI CCAn_nnn'nTGG
Cuts at: 0 199 417 852
Size: 199 218 435
Fragments arranged by size:
435 218 199
PfoI T'CCnGG_A
Cuts at: 0 277 390 399 462 852
Size: 277 113 9 63 390
Fragments arranged by size:
390 277 113 63 9
PpuMI rG'GwC_Cy
Cuts at: 0 574 852
Size: 574 278
SacI G_AGCT'C
Cuts at: 0 286 852
Size: 286 566
SacII CC_GC'GG
Cuts at: 0 332 852
Size: 332 520
SexAI A'CCwGG_T
Cuts at: 0 570 852
Size: 570 282
SfoI GGC'GCC
Cuts at: 0 116 852
Size: 116 736
StyI C'CwwG_G
Cuts at: 0 23 35 149 725 827 852
Size: 23 12 114 576 102 25
Fragments arranged by size:
576 114 102 25 23 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 122 206 445 852
Size: 122 84 239 407
Fragments arranged by size:
407 239 122 84
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 196 578 852
Size: 196 382 274
Fragments arranged by size:
382 274 196
ZraI GAC'GTC
Cuts at: 0 218 852
Size: 218 634
Enzymes that do cut:
AatII AfeI AflIII AlwNI BanI BanII BbeI BglI
BglII Bme1580I BmgBI BpmI BsaBI BsaHI BsaXI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BsrBI BsrDI BstAPI
BstXI BstYI BtgI CspCI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRV HaeII HincII KasI MscI
MspA1I NarI NotI PasI PflMI PfoI PpuMI SacI
SacII SexAI SfoI StyI TaqII XcmI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AcuI AflII AgeI AhdI
AleI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbsI BbvCI BcgI
BciVI BclI BfrBI BlpI BmrI BmtI BplI Bpu10I
BpuEI BsaI BsaAI BsaWI BseRI BsiWI BsmBI BspEI
BspHI BspMI BsrFI BsrGI BssHII BssSI BstBI BstEII
BstZ17I Bsu36I BtgZI BtsI ClaI DraI DraIII DrdI
EcoNI EcoRI FalI FseI FspI FspAI Hin4I HindIII
HpaI KpnI MfeI MluI MmeI MslI NaeI NcoI
NdeI NgoMIV NheI NruI NsiI NspI PacI PciI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI PvuII RsrII SalI SanDI SapI SbfI
ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI TatI TstI
Tth111I XbaI XhoI XmaI XmnI