Restriction map for
pF1KB8929
(Linear) (Six-base) MAPSORT of: KIBB8929.seq Check: 6394 from: 1 to: 843
KIBB8929.orf
With 182 enzymes: SgfI *
March 2, 2010 14:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 470 843
Size: 470 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 571 843
Size: 571 272
AflIII A'CryG_T
Cuts at: 0 95 843
Size: 95 748
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 552 843
Size: 552 291
AlwNI CAG_nnn'CTG
Cuts at: 0 190 495 843
Size: 190 305 348
Fragments arranged by size:
348 305 190
ApaI G_GGCC'C
Cuts at: 0 80 214 843
Size: 80 134 629
Fragments arranged by size:
629 134 80
AvaI C'yCGr_G
Cuts at: 0 32 52 79 371 843
Size: 32 20 27 292 472
Fragments arranged by size:
472 292 32 27 20
BanI G'GyrC_C
Cuts at: 0 555 843
Size: 555 288
BanII G_rGCy'C
Cuts at: 0 32 80 214 371 843
Size: 32 48 134 157 472
Fragments arranged by size:
472 157 134 48 32
BbeI G_GCGC'C
Cuts at: 0 559 843
Size: 559 284
BbvCI CC'TCA_GC
Cuts at: 0 625 843
Size: 625 218
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 154 188 627 661 843
Size: 154 34 439 34 182
Fragments arranged by size:
439 182 154 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 209 375 517 843
Size: 209 166 142 326
Fragments arranged by size:
326 209 166 142
Bme1580I G_kGCm'C
Cuts at: 0 80 214 843
Size: 80 134 629
Fragments arranged by size:
629 134 80
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 709 730 843
Size: 709 21 113
Fragments arranged by size:
709 113 21
Bpu10I CC'TnA_GC
Cuts at: 0 625 843
Size: 625 218
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 490 634 843
Size: 190 300 144 209
Fragments arranged by size:
300 209 190 144
BsaHI Gr'CG_yC
Cuts at: 0 556 843
Size: 556 287
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 379 843
Size: 379 464
BseYI C'CCAG_C
Cuts at: 0 73 303 843
Size: 73 230 540
Fragments arranged by size:
540 230 73
BsmI GAATG_Cn'
Cuts at: 0 683 843
Size: 683 160
BsmBI CGTCTCn'nnnn_
Cuts at: 0 685 843
Size: 685 158
Bsp1286I G_dGCh'C
Cuts at: 0 32 80 214 371 843
Size: 32 48 134 157 472
Fragments arranged by size:
472 157 134 48 32
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 470 843
Size: 470 373
BsrFI r'CCGG_y
Cuts at: 0 620 843
Size: 620 223
BstAPI GCAn_nnn'nTGC
Cuts at: 0 705 843
Size: 705 138
BstXI CCAn_nnnn'nTGG
Cuts at: 0 351 843
Size: 351 492
Bsu36I CC'TnA_GG
Cuts at: 0 191 803 843
Size: 191 612 40
Fragments arranged by size:
612 191 40
BtgI C'CryG_G
Cuts at: 0 6 843
Size: 6 837
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 630 843
Size: 630 213
BtsI GCAGTG_nn'
Cuts at: 0 521 602 843
Size: 521 81 241
Fragments arranged by size:
521 241 81
DraIII CAC_nnn'GTG
Cuts at: 0 442 843
Size: 442 401
EaeI y'GGCC_r
Cuts at: 0 39 486 518 809 843
Size: 39 447 32 291 34
Fragments arranged by size:
447 291 39 34 32
EarI CTCTTCn'nnn_
Cuts at: 0 143 793 843
Size: 143 650 50
Fragments arranged by size:
650 143 50
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 117 781 843
Size: 117 664 62
Fragments arranged by size:
664 117 62
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 571 709 730 843
Size: 571 138 21 113
Fragments arranged by size:
571 138 113 21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 550 820 843
Size: 550 270 23
Fragments arranged by size:
550 270 23
EcoO109I rG'GnC_Cy
Cuts at: 0 11 210 211 307 510 843
Size: 11 199 1 96 203 333
Fragments arranged by size:
333 203 199 96 11 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 172 628 843
Size: 172 456 215
Fragments arranged by size:
456 215 172
FspI TGC'GCA
Cuts at: 0 655 843
Size: 655 188
HaeII r_GCGC'y
Cuts at: 0 190 304 559 735 843
Size: 190 114 255 176 108
Fragments arranged by size:
255 190 176 114 108
KasI G'GCGC_C
Cuts at: 0 555 843
Size: 555 288
MscI TGG'CCA
Cuts at: 0 41 520 843
Size: 41 479 323
Fragments arranged by size:
479 323 41
MslI CAynn'nnrTG
Cuts at: 0 649 843
Size: 649 194
NarI GG'CG_CC
Cuts at: 0 556 843
Size: 556 287
NspI r_CATG'y
Cuts at: 0 99 843
Size: 99 744
PasI CC'CwG_GG
Cuts at: 0 328 344 843
Size: 328 16 499
Fragments arranged by size:
499 328 16
PciI A'CATG_T
Cuts at: 0 95 843
Size: 95 748
PpuMI rG'GwC_Cy
Cuts at: 0 11 843
Size: 11 832
PspOMI G'GGCC_C
Cuts at: 0 76 210 843
Size: 76 134 633
Fragments arranged by size:
633 134 76
PspXI vC'TCGA_Gb
Cuts at: 0 52 843
Size: 52 791
PstI C_TGCA'G
Cuts at: 0 425 541 843
Size: 425 116 302
Fragments arranged by size:
425 302 116
SapI GCTCTTCn'nnn_
Cuts at: 0 143 843
Size: 143 700
SfcI C'TryA_G
Cuts at: 0 318 421 537 843
Size: 318 103 116 306
Fragments arranged by size:
318 306 116 103
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 209 517 843
Size: 209 308 326
Fragments arranged by size:
326 308 209
SfoI GGC'GCC
Cuts at: 0 557 843
Size: 557 286
SmaI CCC'GGG
Cuts at: 0 34 81 373 843
Size: 34 47 292 470
Fragments arranged by size:
470 292 47 34
SmlI C'TyrA_G
Cuts at: 0 52 205 505 613 843
Size: 52 153 300 108 230
Fragments arranged by size:
300 230 153 108 52
StuI AGG'CCT
Cuts at: 0 112 203 843
Size: 112 91 640
Fragments arranged by size:
640 112 91
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 775 843
Size: 775 68
TatI w'GTAC_w
Cuts at: 0 713 843
Size: 713 130
XhoI C'TCGA_G
Cuts at: 0 52 843
Size: 52 791
XmaI C'CCGG_G
Cuts at: 0 32 79 371 843
Size: 32 47 292 472
Fragments arranged by size:
472 292 47 32
Enzymes that do cut:
AarI AcuI AflIII AlfI AlwNI ApaI AvaI BanI
BanII BbeI BbvCI BcgI BglI Bme1580I BpmI Bpu10I
BpuEI BsaHI BseRI BseYI BsmI BsmBI Bsp1286I BspMI
BsrFI BstAPI BstXI Bsu36I BtgI BtgZI BtsI DraIII
EaeI EarI EciI Eco57MI EcoNI EcoO109I FalI FspI
HaeII KasI MscI MslI NarI NspI PasI PciI
PpuMI PspOMI PspXI PstI SapI SfcI SfiI SfoI
SmaI SmlI StuI TaqII TatI XhoI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbsI BciVI BclI BfrBI BglII BlpI
BmgBI BmrI BmtI BplI BsaI BsaAI BsaBI BsaWI
BsaXI BsgI BsiEI BsiHKAI BsiWI BspEI BspHI BsrBI
BsrDI BsrGI BssHII BssSI BstBI BstEII BstYI BstZ17I
ClaI CspCI DraI DrdI EagI EcoICRI EcoRI EcoRV
FseI FspAI Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PflMI PfoI PmeI PmlI
PpiI PshAI PsiI PsrI PvuI PvuII RsrII SacI
SacII SalI SanDI SbfI ScaI SexAI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI StyI SwaI TstI
Tth111I XbaI XcmI XmnI ZraI