Restriction map for
pF1KB8926
(Linear) (Six-base) MAPSORT of: KIBB8926.seq Check: 7314 from: 1 to: 819
KIBB8926.orf
With 182 enzymes: SgfI *
March 3, 2008 11:09 ..
AclI AA'CG_TT
Cuts at: 0 277 819
Size: 277 542
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 101 324 408 492 576 661 819
Size: 101 223 84 84 84 85 158
Fragments arranged by size:
223 158 101 85 84 84 84
AhdI GACnn_n'nnGTC
Cuts at: 0 223 819
Size: 223 596
AleI CACnn'nnGTG
Cuts at: 0 551 819
Size: 551 268
ApaLI G'TGCA_C
Cuts at: 0 318 784 819
Size: 318 466 35
Fragments arranged by size:
466 318 35
ApoI r'AATT_y
Cuts at: 0 141 819
Size: 141 678
BamHI G'GATC_C
Cuts at: 0 118 819
Size: 118 701
BbsI GAAGACnn'nnnn_
Cuts at: 0 193 819
Size: 193 626
BciVI GTATCCnnnnn_n'
Cuts at: 0 368 819
Size: 368 451
BclI T'GATC_A
Cuts at: 0 573 819
Size: 573 246
Bme1580I G_kGCm'C
Cuts at: 0 322 788 819
Size: 322 466 31
Fragments arranged by size:
466 322 31
BmrI ACTGGGnnnn_n'
Cuts at: 0 654 819
Size: 654 165
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 457 819
Size: 457 362
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 230 819
Size: 230 589
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 108 819
Size: 108 711
BseYI C'CCAG_C
Cuts at: 0 128 819
Size: 128 691
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 684 819
Size: 684 135
BsiHKAI G_wGCw'C
Cuts at: 0 322 788 819
Size: 322 466 31
Fragments arranged by size:
466 322 31
Bsp1286I G_dGCh'C
Cuts at: 0 322 788 819
Size: 322 466 31
Fragments arranged by size:
466 322 31
BssSI C'ACGA_G
Cuts at: 0 451 819
Size: 451 368
BstBI TT'CG_AA
Cuts at: 0 184 819
Size: 184 635
BstXI CCAn_nnnn'nTGG
Cuts at: 0 244 819
Size: 244 575
BstYI r'GATC_y
Cuts at: 0 118 819
Size: 118 701
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 393 428 819
Size: 393 35 391
Fragments arranged by size:
393 391 35
EaeI y'GGCC_r
Cuts at: 0 741 819
Size: 741 78
EarI CTCTTCn'nnn_
Cuts at: 0 147 664 819
Size: 147 517 155
Fragments arranged by size:
517 155 147
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 101 230 324 408 492 576 661 819
Size: 101 129 94 84 84 84 85 158
Fragments arranged by size:
158 129 101 94 85 84 84 84
EcoRV GAT'ATC
Cuts at: 0 135 819
Size: 135 684
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 373 819
Size: 373 446
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 209 241 717 749 759 791 819
Size: 209 32 476 32 10 32 28
Fragments arranged by size:
476 209 32 32 32 28 10
HincII GTy'rAC
Cuts at: 0 430 819
Size: 430 389
HindIII A'AGCT_T
Cuts at: 0 336 420 504 606 756 819
Size: 336 84 84 102 150 63
Fragments arranged by size:
336 150 102 84 84 63
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 819
Size: 37 782
MslI CAynn'nnrTG
Cuts at: 0 95 551 819
Size: 95 456 268
Fragments arranged by size:
456 268 95
SfcI C'TryA_G
Cuts at: 0 113 193 564 819
Size: 113 80 371 255
Fragments arranged by size:
371 255 113 80
SmlI C'TyrA_G
Cuts at: 0 123 819
Size: 123 696
StuI AGG'CCT
Cuts at: 0 638 819
Size: 638 181
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 337 778 819
Size: 337 441 41
Fragments arranged by size:
441 337 41
XmnI GAAnn'nnTTC
Cuts at: 0 421 673 819
Size: 421 252 146
Fragments arranged by size:
421 252 146
Enzymes that do cut:
AclI AcuI AhdI AleI ApaLI ApoI BamHI BbsI
BciVI BclI Bme1580I BmrI BplI BpmI BpuEI BseYI
BsgI BsiHKAI Bsp1286I BssSI BstBI BstXI BstYI CspCI
EaeI EarI Eco57MI EcoRV FalI Hin4I HincII HindIII
MmeI MslI SfcI SmlI StuI TaqII XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AflIII AgeI
AlfI AloI AlwNI ApaI AscI AseI AsiSI AvaI
AvrII BaeI BanI BanII BbeI BbvCI BcgI BfrBI
BglI BglII BlpI BmgBI BmtI Bpu10I BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BstAPI BstEII BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI DraI DraIII DrdI EagI EciI EcoICRI EcoNI
EcoO109I EcoRI FseI FspI FspAI HaeII HpaI KasI
KpnI MfeI MluI MscI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StyI SwaI TatI TstI Tth111I
XbaI XcmI XhoI XmaI ZraI