Restriction map for
pF1KB8925
(Linear) (Six-base) MAPSORT of: KIBB8925.seq Check: 6989 from: 1 to: 819
KIBB8925.orf
With 182 enzymes: SgfI *
March 3, 2008 11:09 ..
Acc65I G'GTAC_C
Cuts at: 0 452 819
Size: 452 367
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 232 819
Size: 202 30 587
Fragments arranged by size:
587 202 30
AlwNI CAG_nnn'CTG
Cuts at: 0 174 666 723 819
Size: 174 492 57 96
Fragments arranged by size:
492 174 96 57
ApaI G_GGCC'C
Cuts at: 0 116 819
Size: 116 703
AvaI C'yCGr_G
Cuts at: 0 115 354 466 557 819
Size: 115 239 112 91 262
Fragments arranged by size:
262 239 115 112 91
BanI G'GyrC_C
Cuts at: 0 151 279 452 462 819
Size: 151 128 173 10 357
Fragments arranged by size:
357 173 151 128 10
BanII G_rGCy'C
Cuts at: 0 79 116 317 819
Size: 79 37 201 502
Fragments arranged by size:
502 201 79 37
BbeI G_GCGC'C
Cuts at: 0 155 283 466 819
Size: 155 128 183 353
Fragments arranged by size:
353 183 155 128
BglI GCCn_nnn'nGGC
Cuts at: 0 130 144 819
Size: 130 14 675
Fragments arranged by size:
675 130 14
BglII A'GATC_T
Cuts at: 0 518 819
Size: 518 301
Bme1580I G_kGCm'C
Cuts at: 0 116 617 819
Size: 116 501 202
Fragments arranged by size:
501 202 116
BmrI ACTGGGnnnn_n'
Cuts at: 0 741 765 819
Size: 741 24 54
Fragments arranged by size:
741 54 24
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 93 819
Size: 93 726
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 448 819
Size: 448 371
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 279 819
Size: 279 540
BsaI GGTCTCn'nnnn_
Cuts at: 0 343 819
Size: 343 476
BsaHI Gr'CG_yC
Cuts at: 0 152 280 371 463 583 819
Size: 152 128 91 92 120 236
Fragments arranged by size:
236 152 128 120 92 91
BsaWI w'CCGG_w
Cuts at: 0 93 333 819
Size: 93 240 486
Fragments arranged by size:
486 240 93
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 283 313 819
Size: 283 30 506
Fragments arranged by size:
506 283 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 85 819
Size: 85 734
BseYI C'CCAG_C
Cuts at: 0 141 803 819
Size: 141 662 16
Fragments arranged by size:
662 141 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 244 819
Size: 187 57 575
Fragments arranged by size:
575 187 57
BsiEI CG_ry'CG
Cuts at: 0 323 819
Size: 323 496
BsmI GAATG_Cn'
Cuts at: 0 692 819
Size: 692 127
Bsp1286I G_dGCh'C
Cuts at: 0 79 116 317 617 819
Size: 79 37 201 300 202
Fragments arranged by size:
300 202 201 79 37
BspEI T'CCGG_A
Cuts at: 0 93 819
Size: 93 726
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 59 501 819
Size: 59 442 318
Fragments arranged by size:
442 318 59
BsrBI CCG'CTC
Cuts at: 0 432 819
Size: 432 387
BsrFI r'CCGG_y
Cuts at: 0 373 819
Size: 373 446
BssHII G'CGCG_C
Cuts at: 0 552 713 819
Size: 552 161 106
Fragments arranged by size:
552 161 106
BstAPI GCAn_nnn'nTGC
Cuts at: 0 174 723 732 819
Size: 174 549 9 87
Fragments arranged by size:
549 174 87 9
BstYI r'GATC_y
Cuts at: 0 518 819
Size: 518 301
Bsu36I CC'TnA_GG
Cuts at: 0 287 819
Size: 287 532
BtsI GCAGTG_nn'
Cuts at: 0 814 819
Size: 814 5
EaeI y'GGCC_r
Cuts at: 0 83 230 320 482 608 758 819
Size: 83 147 90 162 126 150 61
Fragments arranged by size:
162 150 147 126 90 83 61
EagI C'GGCC_G
Cuts at: 0 320 819
Size: 320 499
EarI CTCTTCn'nnn_
Cuts at: 0 95 819
Size: 95 724
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 484 819
Size: 484 335
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 232 448 819
Size: 202 30 216 371
Fragments arranged by size:
371 216 202 30
EcoNI CCTnn'n_nnAGG
Cuts at: 0 796 819
Size: 796 23
EcoO109I rG'GnC_Cy
Cuts at: 0 112 243 663 819
Size: 112 131 420 156
Fragments arranged by size:
420 156 131 112
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 60 207 819
Size: 60 147 612
Fragments arranged by size:
612 147 60
HaeII r_GCGC'y
Cuts at: 0 155 283 438 466 819
Size: 155 128 155 28 353
Fragments arranged by size:
353 155 155 128 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 283 315 819
Size: 283 32 504
Fragments arranged by size:
504 283 32
KasI G'GCGC_C
Cuts at: 0 151 279 462 819
Size: 151 128 183 357
Fragments arranged by size:
357 183 151 128
KpnI G_GTAC'C
Cuts at: 0 456 819
Size: 456 363
MscI TGG'CCA
Cuts at: 0 232 484 819
Size: 232 252 335
Fragments arranged by size:
335 252 232
MspA1I CmG'CkG
Cuts at: 0 141 450 819
Size: 141 309 369
Fragments arranged by size:
369 309 141
NaeI GCC'GGC
Cuts at: 0 375 819
Size: 375 444
NarI GG'CG_CC
Cuts at: 0 152 280 463 819
Size: 152 128 183 356
Fragments arranged by size:
356 183 152 128
NgoMIV G'CCGG_C
Cuts at: 0 373 819
Size: 373 446
NspI r_CATG'y
Cuts at: 0 735 819
Size: 735 84
PflMI CCAn_nnn'nTGG
Cuts at: 0 666 819
Size: 666 153
PpuMI rG'GwC_Cy
Cuts at: 0 663 819
Size: 663 156
PspOMI G'GGCC_C
Cuts at: 0 112 819
Size: 112 707
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 178 210 523 555 819
Size: 178 32 313 32 264
Fragments arranged by size:
313 264 178 32 32
PstI C_TGCA'G
Cuts at: 0 728 819
Size: 728 91
ScaI AGT'ACT
Cuts at: 0 253 819
Size: 253 566
SfcI C'TryA_G
Cuts at: 0 708 724 819
Size: 708 16 95
Fragments arranged by size:
708 95 16
SfoI GGC'GCC
Cuts at: 0 153 281 464 819
Size: 153 128 183 355
Fragments arranged by size:
355 183 153 128
SmaI CCC'GGG
Cuts at: 0 356 559 819
Size: 356 203 260
Fragments arranged by size:
356 260 203
SmlI C'TyrA_G
Cuts at: 0 294 819
Size: 294 525
SrfI GCCC'GGGC
Cuts at: 0 559 819
Size: 559 260
StyI C'CwwG_G
Cuts at: 0 407 819
Size: 407 412
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 21 819
Size: 21 798
TatI w'GTAC_w
Cuts at: 0 251 737 749 819
Size: 251 486 12 70
Fragments arranged by size:
486 251 70 12
XmaI C'CCGG_G
Cuts at: 0 354 557 819
Size: 354 203 262
Fragments arranged by size:
354 262 203
XmnI GAAnn'nnTTC
Cuts at: 0 190 819
Size: 190 629
Enzymes that do cut:
Acc65I AcuI AlwNI ApaI AvaI BanI BanII BbeI
BglI BglII Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsmI
Bsp1286I BspEI BspMI BsrBI BsrFI BssHII BstAPI BstYI
Bsu36I BtsI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I FalI HaeII Hin4I KasI KpnI MscI MspA1I
NaeI NarI NgoMIV NspI PflMI PpuMI PspOMI PsrI
PstI ScaI SfcI SfoI SmaI SmlI SrfI StyI
TaqII TatI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI BciVI
BclI BfrBI BlpI BmgBI BmtI Bpu10I BsaAI BsaBI
BsiHKAI BsiWI BsmBI BspHI BsrDI BsrGI BssSI BstBI
BstEII BstXI BstZ17I BtgI BtgZI ClaI CspCI DraI
DraIII DrdI EcoICRI EcoRI EcoRV FseI FspI FspAI
HincII HindIII HpaI MfeI MluI MmeI MslI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PciI
PfoI PmeI PmlI PpiI PshAI PsiI PspXI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SspI
StuI SwaI TstI Tth111I XbaI XcmI XhoI ZraI