Restriction map for
pF1KB8923
(Linear) (Six-base) MAPSORT of: KIBB8923.seq Check: 6149 from: 1 to: 810
KIBB8923.orf
With 182 enzymes: SgfI *
March 2, 2010 14:30 ..
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 497 810
Size: 497 313
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 622 654 810
Size: 622 32 156
Fragments arranged by size:
622 156 32
AlwNI CAG_nnn'CTG
Cuts at: 0 621 810
Size: 621 189
ApaI G_GGCC'C
Cuts at: 0 594 810
Size: 594 216
AvaI C'yCGr_G
Cuts at: 0 174 810
Size: 174 636
BanI G'GyrC_C
Cuts at: 0 237 379 504 810
Size: 237 142 125 306
Fragments arranged by size:
306 237 142 125
BanII G_rGCy'C
Cuts at: 0 7 181 251 502 594 810
Size: 7 174 70 251 92 216
Fragments arranged by size:
251 216 174 92 70 7
BbeI G_GCGC'C
Cuts at: 0 241 383 508 810
Size: 241 142 125 302
Fragments arranged by size:
302 241 142 125
BbvCI CC'TCA_GC
Cuts at: 0 287 810
Size: 287 523
BglI GCCn_nnn'nGGC
Cuts at: 0 176 191 235 347 581 598 810
Size: 176 15 44 112 234 17 212
Fragments arranged by size:
234 212 176 112 44 17 15
BglII A'GATC_T
Cuts at: 0 524 810
Size: 524 286
Bme1580I G_kGCm'C
Cuts at: 0 594 810
Size: 594 216
BmgBI CAC'GTC
Cuts at: 0 272 810
Size: 272 538
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 357 643 649 810
Size: 357 286 6 161
Fragments arranged by size:
357 286 161 6
Bpu10I CC'TnA_GC
Cuts at: 0 287 364 810
Size: 287 77 446
Fragments arranged by size:
446 287 77
BsaHI Gr'CG_yC
Cuts at: 0 238 380 392 436 455 505 810
Size: 238 142 12 44 19 50 305
Fragments arranged by size:
305 238 142 50 44 19 12
BsaWI w'CCGG_w
Cuts at: 0 196 439 734 810
Size: 196 243 295 76
Fragments arranged by size:
295 243 196 76
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 178 208 267 297 622 652 810
Size: 178 30 59 30 325 30 158
Fragments arranged by size:
325 178 158 59 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 275 463 810
Size: 275 188 347
Fragments arranged by size:
347 275 188
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 532 810
Size: 532 278
BsiEI CG_ry'CG
Cuts at: 0 33 171 389 810
Size: 33 138 218 421
Fragments arranged by size:
421 218 138 33
BsiHKAI G_wGCw'C
Cuts at: 0 7 810
Size: 7 803
Bsp1286I G_dGCh'C
Cuts at: 0 7 181 251 502 594 810
Size: 7 174 70 251 92 216
Fragments arranged by size:
251 216 174 92 70 7
BspEI T'CCGG_A
Cuts at: 0 196 439 810
Size: 196 243 371
Fragments arranged by size:
371 243 196
BspHI T'CATG_A
Cuts at: 0 558 810
Size: 558 252
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 119 810
Size: 119 691
BsrBI CCG'CTC
Cuts at: 0 173 810
Size: 173 637
BsrFI r'CCGG_y
Cuts at: 0 264 394 810
Size: 264 130 416
Fragments arranged by size:
416 264 130
BssHII G'CGCG_C
Cuts at: 0 204 810
Size: 204 606
BstYI r'GATC_y
Cuts at: 0 156 524 810
Size: 156 368 286
Fragments arranged by size:
368 286 156
BtgI C'CryG_G
Cuts at: 0 314 345 785 810
Size: 314 31 440 25
Fragments arranged by size:
440 314 31 25
EaeI y'GGCC_r
Cuts at: 0 168 183 266 386 782 810
Size: 168 15 83 120 396 28
Fragments arranged by size:
396 168 120 83 28 15
EagI C'GGCC_G
Cuts at: 0 168 386 810
Size: 168 218 424
Fragments arranged by size:
424 218 168
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 300 810
Size: 300 510
EcoICRI GAG'CTC
Cuts at: 0 5 810
Size: 5 805
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 357 643 649 810
Size: 357 286 6 161
Fragments arranged by size:
357 286 161 6
EcoO109I rG'GnC_Cy
Cuts at: 0 590 797 810
Size: 590 207 13
Fragments arranged by size:
590 207 13
FspI TGC'GCA
Cuts at: 0 485 541 810
Size: 485 56 269
Fragments arranged by size:
485 269 56
HaeII r_GCGC'y
Cuts at: 0 230 241 383 508 810
Size: 230 11 142 125 302
Fragments arranged by size:
302 230 142 125 11
KasI G'GCGC_C
Cuts at: 0 237 379 504 810
Size: 237 142 125 306
Fragments arranged by size:
306 237 142 125
MslI CAynn'nnrTG
Cuts at: 0 68 778 810
Size: 68 710 32
Fragments arranged by size:
710 68 32
MspA1I CmG'CkG
Cuts at: 0 300 377 787 810
Size: 300 77 410 23
Fragments arranged by size:
410 300 77 23
NaeI GCC'GGC
Cuts at: 0 266 396 810
Size: 266 130 414
Fragments arranged by size:
414 266 130
NarI GG'CG_CC
Cuts at: 0 238 380 505 810
Size: 238 142 125 305
Fragments arranged by size:
305 238 142 125
NgoMIV G'CCGG_C
Cuts at: 0 264 394 810
Size: 264 130 416
Fragments arranged by size:
416 264 130
NspI r_CATG'y
Cuts at: 0 120 691 810
Size: 120 571 119
Fragments arranged by size:
571 120 119
PflMI CCAn_nnn'nTGG
Cuts at: 0 621 810
Size: 621 189
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 622 654 810
Size: 622 32 156
Fragments arranged by size:
622 156 32
PspOMI G'GGCC_C
Cuts at: 0 590 810
Size: 590 220
SacI G_AGCT'C
Cuts at: 0 7 810
Size: 7 803
SacII CC_GC'GG
Cuts at: 0 788 810
Size: 788 22
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 598 810
Size: 598 212
SfoI GGC'GCC
Cuts at: 0 239 381 506 810
Size: 239 142 125 304
Fragments arranged by size:
304 239 142 125
SphI G_CATG'C
Cuts at: 0 120 810
Size: 120 690
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 418 810
Size: 418 392
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 178 210 267 299 810
Size: 178 32 57 32 511
Fragments arranged by size:
511 178 57 32 32
XmnI GAAnn'nnTTC
Cuts at: 0 637 810
Size: 637 173
Enzymes that do cut:
AlfI AloI AlwNI ApaI AvaI BanI BanII BbeI
BbvCI BglI BglII Bme1580I BmgBI BpmI Bpu10I BsaHI
BsaWI BsaXI BseRI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrFI BssHII BstYI BtgI EaeI
EagI EciI EcoICRI Eco57MI EcoO109I FspI HaeII KasI
MslI MspA1I NaeI NarI NgoMIV NspI PflMI PpiI
PspOMI SacI SacII SfiI SfoI SphI TaqII TstI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbsI BcgI BciVI BclI
BfrBI BlpI BmrI BmtI BplI BpuEI BsaI BsaAI
BsaBI BseYI BsiWI BsmI BsmBI BsrDI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EarI EcoNI EcoRI
EcoRV FalI FseI FspAI Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PciI PfoI PmeI
PmlI PpuMI PshAI PsiI PspXI PsrI PstI PvuI
PvuII RsrII SalI SanDI SapI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI
SspI StuI StyI SwaI TatI Tth111I XbaI XcmI
XhoI XmaI ZraI