(Linear) (Six-base) MAPSORT of: KIBB8918.seq  Check: 899  from: 1  to: 786



KIBB8918.orf

 With 182 enzymes: SgfI * 

                        October 2, 2008 10:57  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     671     786 
   Size:        671     115
 
ApaLI G'TGCA_C

Cuts at:      0     587     786 
   Size:        587     199
 
ApoI r'AATT_y

Cuts at:      0     138     277     285     389     405     473     786 
   Size:        138     139       8     104      16      68     313

  Fragments arranged by size: 

                 313     139     138     104      68      16       8
 
BfrBI ATG'CAT

Cuts at:      0     273     786 
   Size:        273     513
 
Bme1580I G_kGCm'C

Cuts at:      0     591     786 
   Size:        591     195
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     503     587     755     786 
   Size:        503      84     168      31

  Fragments arranged by size: 

                 503     168      84      31
 
BsiHKAI G_wGCw'C

Cuts at:      0     591     786 
   Size:        591     195
 
Bsp1286I G_dGCh'C

Cuts at:      0     591     786 
   Size:        591     195
 
DraI TTT'AAA

Cuts at:      0     609     786 
   Size:        609     177
 
DraIII CAC_nnn'GTG

Cuts at:      0     595     786 
   Size:        595     191
 
EarI CTCTTCn'nnn_

Cuts at:      0     416     647     786 
   Size:        416     231     139

  Fragments arranged by size: 

                 416     231     139
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     503     587     671     755     786 
   Size:        503      84      84      84      31

  Fragments arranged by size: 

                 503      84      84      84      31
 
HindIII A'AGCT_T

Cuts at:      0     770     786 
   Size:        770      16
 
MslI CAynn'nnrTG

Cuts at:      0     750     786 
   Size:        750      36
 
NsiI A_TGCA'T

Cuts at:      0     275     786 
   Size:        275     511
 
NspI r_CATG'y

Cuts at:      0     363     786 
   Size:        363     423
 
PsiI TTA'TAA

Cuts at:      0     200     786 
   Size:        200     586
 
SphI G_CATG'C

Cuts at:      0     363     786 
   Size:        363     423
 
SspI AAT'ATT

Cuts at:      0     242     786 
   Size:        242     544
 
StyI C'CwwG_G

Cuts at:      0     189     786 
   Size:        189     597
 

 Enzymes that do cut:

     AcuI    ApaLI     ApoI    BfrBI Bme1580I     BpmI  BsiHKAI Bsp1286I
     DraI   DraIII     EarI  Eco57MI  HindIII     MslI     NsiI     NspI
     PsiI     SphI     SspI     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI     BglI    BglII
     BlpI    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I
   HincII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SrfI     StuI     SwaI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI