Restriction map for
pF1KB8914
(Linear) (Six-base) MAPSORT of: KIBB8914.seq Check: 3712 from: 1 to: 765
KIBB8914.orf
With 182 enzymes: SgfI *
March 3, 2008 11:09 ..
AccI GT'mk_AC
Cuts at: 0 398 765
Size: 398 367
AgeI A'CCGG_T
Cuts at: 0 448 765
Size: 448 317
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 31 63 765
Size: 31 32 702
Fragments arranged by size:
702 32 31
ApaLI G'TGCA_C
Cuts at: 0 418 765
Size: 418 347
ApoI r'AATT_y
Cuts at: 0 426 514 765
Size: 426 88 251
Fragments arranged by size:
426 251 88
AvaI C'yCGr_G
Cuts at: 0 144 199 329 367 765
Size: 144 55 130 38 398
Fragments arranged by size:
398 144 130 55 38
AvrII C'CTAG_G
Cuts at: 0 84 765
Size: 84 681
BanI G'GyrC_C
Cuts at: 0 97 300 312 325 542 765
Size: 97 203 12 13 217 223
Fragments arranged by size:
223 217 203 97 13 12
BanII G_rGCy'C
Cuts at: 0 152 765
Size: 152 613
BbeI G_GCGC'C
Cuts at: 0 101 304 316 329 546 765
Size: 101 203 12 13 217 219
Fragments arranged by size:
219 217 203 101 13 12
BglI GCCn_nnn'nGGC
Cuts at: 0 147 765
Size: 147 618
BglII A'GATC_T
Cuts at: 0 596 765
Size: 596 169
Bme1580I G_kGCm'C
Cuts at: 0 422 765
Size: 422 343
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 257 765
Size: 257 508
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 364 765
Size: 364 401
BsaAI yAC'GTr
Cuts at: 0 423 765
Size: 423 342
BsaBI GATnn'nnATC
Cuts at: 0 556 595 765
Size: 556 39 170
Fragments arranged by size:
556 170 39
BsaHI Gr'CG_yC
Cuts at: 0 98 301 313 326 543 765
Size: 98 203 12 13 217 222
Fragments arranged by size:
222 217 203 98 13 12
BsaWI w'CCGG_w
Cuts at: 0 448 611 765
Size: 448 163 154
Fragments arranged by size:
448 163 154
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 76 672 684 687 765
Size: 76 596 12 3 78
Fragments arranged by size:
596 78 76 12 3
BseYI C'CCAG_C
Cuts at: 0 288 709 765
Size: 288 421 56
Fragments arranged by size:
421 288 56
BsiEI CG_ry'CG
Cuts at: 0 392 765
Size: 392 373
BsiHKAI G_wGCw'C
Cuts at: 0 422 765
Size: 422 343
Bsp1286I G_dGCh'C
Cuts at: 0 152 422 765
Size: 152 270 343
Fragments arranged by size:
343 270 152
BspHI T'CATG_A
Cuts at: 0 5 765
Size: 5 760
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 650 765
Size: 650 115
BsrBI CCG'CTC
Cuts at: 0 271 765
Size: 271 494
BsrFI r'CCGG_y
Cuts at: 0 309 387 448 765
Size: 309 78 61 317
Fragments arranged by size:
317 309 78 61
BssHII G'CGCG_C
Cuts at: 0 119 765
Size: 119 646
BstXI CCAn_nnnn'nTGG
Cuts at: 0 36 765
Size: 36 729
BstYI r'GATC_y
Cuts at: 0 596 765
Size: 596 169
BtgI C'CryG_G
Cuts at: 0 157 392 765
Size: 157 235 373
Fragments arranged by size:
373 235 157
DraIII CAC_nnn'GTG
Cuts at: 0 572 765
Size: 572 193
EaeI y'GGCC_r
Cuts at: 0 389 731 765
Size: 389 342 34
Fragments arranged by size:
389 342 34
EagI C'GGCC_G
Cuts at: 0 389 765
Size: 389 376
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 296 765
Size: 296 469
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 257 765
Size: 257 508
EcoNI CCTnn'n_nnAGG
Cuts at: 0 185 765
Size: 185 580
EcoRI G'AATT_C
Cuts at: 0 426 765
Size: 426 339
HaeII r_GCGC'y
Cuts at: 0 101 217 304 316 329 546 588 765
Size: 101 116 87 12 13 217 42 177
Fragments arranged by size:
217 177 116 101 87 42 13 12
KasI G'GCGC_C
Cuts at: 0 97 300 312 325 542 765
Size: 97 203 12 13 217 223
Fragments arranged by size:
223 217 203 97 13 12
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 371 529 560 765
Size: 371 158 31 205
Fragments arranged by size:
371 205 158 31
MscI TGG'CCA
Cuts at: 0 733 765
Size: 733 32
MspA1I CmG'CkG
Cuts at: 0 194 765
Size: 194 571
NaeI GCC'GGC
Cuts at: 0 311 389 765
Size: 311 78 376
Fragments arranged by size:
376 311 78
NarI GG'CG_CC
Cuts at: 0 98 301 313 326 543 765
Size: 98 203 12 13 217 222
Fragments arranged by size:
222 217 203 98 13 12
NgoMIV G'CCGG_C
Cuts at: 0 309 387 765
Size: 309 78 378
Fragments arranged by size:
378 309 78
PfoI T'CCnGG_A
Cuts at: 0 273 765
Size: 273 492
PmlI CAC'GTG
Cuts at: 0 423 765
Size: 423 342
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 427 459 765
Size: 427 32 306
Fragments arranged by size:
427 306 32
SfcI C'TryA_G
Cuts at: 0 336 765
Size: 336 429
SfoI GGC'GCC
Cuts at: 0 99 302 314 327 544 765
Size: 99 203 12 13 217 221
Fragments arranged by size:
221 217 203 99 13 12
SgrAI Cr'CCGG_yG
Cuts at: 0 309 448 765
Size: 309 139 317
Fragments arranged by size:
317 309 139
SmaI CCC'GGG
Cuts at: 0 146 201 331 369 765
Size: 146 55 130 38 396
Fragments arranged by size:
396 146 130 55 38
SmlI C'TyrA_G
Cuts at: 0 379 765
Size: 379 386
SrfI GCCC'GGGC
Cuts at: 0 331 765
Size: 331 434
StyI C'CwwG_G
Cuts at: 0 84 765
Size: 84 681
XmaI C'CCGG_G
Cuts at: 0 144 199 329 367 765
Size: 144 55 130 38 398
Fragments arranged by size:
398 144 130 55 38
Enzymes that do cut:
AccI AgeI AloI ApaLI ApoI AvaI AvrII BanI
BanII BbeI BglI BglII Bme1580I BpmI BpuEI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspHI BspMI BsrBI BsrFI BssHII BstXI BstYI BtgI
DraIII EaeI EagI EciI Eco57MI EcoNI EcoRI HaeII
KasI MmeI MscI MspA1I NaeI NarI NgoMIV PfoI
PmlI PsrI SfcI SfoI SgrAI SmaI SmlI SrfI
StyI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AflIII
AhdI AleI AlfI AlwNI ApaI AscI AseI AsiSI
BaeI BamHI BbsI BbvCI BcgI BciVI BclI BfrBI
BlpI BmgBI BmrI BmtI BplI Bpu10I BsaI BsaXI
BsgI BsiWI BsmI BsmBI BspEI BsrDI BsrGI BssSI
BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI BtsI ClaI
CspCI DraI DrdI EarI EcoICRI EcoO109I EcoRV FalI
FseI FspI FspAI Hin4I HincII HindIII HpaI KpnI
MfeI MluI MslI NcoI NdeI NheI NotI NruI
NsiI NspI PacI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SgfI SnaBI SpeI SphI SspI StuI
SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmnI ZraI