Restriction map for
pF1KB8901
(Linear) (Six-base) MAPSORT of: KIBB8901.seq Check: 9731 from: 1 to: 699
KIBB8901.orf
With 182 enzymes: SgfI *
March 3, 2008 11:09 ..
AccI GT'mk_AC
Cuts at: 0 464 699
Size: 464 235
Acc65I G'GTAC_C
Cuts at: 0 299 699
Size: 299 400
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 672 699
Size: 672 27
AfeI AGC'GCT
Cuts at: 0 266 699
Size: 266 433
AvaI C'yCGr_G
Cuts at: 0 230 699
Size: 230 469
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 283 316 557 590 699
Size: 283 33 241 33 109
Fragments arranged by size:
283 241 109 33 33
BamHI G'GATC_C
Cuts at: 0 542 699
Size: 542 157
BanI G'GyrC_C
Cuts at: 0 170 299 699
Size: 170 129 400
Fragments arranged by size:
400 170 129
BanII G_rGCy'C
Cuts at: 0 682 699
Size: 682 17
BbvCI CC'TCA_GC
Cuts at: 0 268 699
Size: 268 431
BciVI GTATCCnnnnn_n'
Cuts at: 0 55 558 699
Size: 55 503 141
Fragments arranged by size:
503 141 55
BglI GCCn_nnn'nGGC
Cuts at: 0 614 699
Size: 614 85
Bme1580I G_kGCm'C
Cuts at: 0 447 699
Size: 447 252
BmrI ACTGGGnnnn_n'
Cuts at: 0 137 417 699
Size: 137 280 282
Fragments arranged by size:
282 280 137
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 90 699
Size: 90 609
Bpu10I CC'TnA_GC
Cuts at: 0 268 699
Size: 268 431
BsaI GGTCTCn'nnnn_
Cuts at: 0 130 699
Size: 130 569
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 22 52 699
Size: 22 30 647
Fragments arranged by size:
647 30 22
BseYI C'CCAG_C
Cuts at: 0 329 560 675 699
Size: 329 231 115 24
Fragments arranged by size:
329 231 115 24
BsmI GAATG_Cn'
Cuts at: 0 310 699
Size: 310 389
Bsp1286I G_dGCh'C
Cuts at: 0 447 682 699
Size: 447 235 17
Fragments arranged by size:
447 235 17
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 468 699
Size: 468 231
BsrFI r'CCGG_y
Cuts at: 0 302 661 699
Size: 302 359 38
Fragments arranged by size:
359 302 38
BstYI r'GATC_y
Cuts at: 0 542 699
Size: 542 157
BtgI C'CryG_G
Cuts at: 0 188 245 699
Size: 188 57 454
Fragments arranged by size:
454 188 57
EaeI y'GGCC_r
Cuts at: 0 207 509 659 699
Size: 207 302 150 40
Fragments arranged by size:
302 207 150 40
EarI CTCTTCn'nnn_
Cuts at: 0 86 394 691 699
Size: 86 308 297 8
Fragments arranged by size:
308 297 86 8
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 90 672 699
Size: 90 582 27
Fragments arranged by size:
582 90 27
EcoO109I rG'GnC_Cy
Cuts at: 0 108 699
Size: 108 591
HaeII r_GCGC'y
Cuts at: 0 268 699
Size: 268 431
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 22 54 310 342 374 699
Size: 22 32 256 32 32 325
Fragments arranged by size:
325 256 32 32 32 22
HincII GTy'rAC
Cuts at: 0 30 699
Size: 30 669
HindIII A'AGCT_T
Cuts at: 0 46 699
Size: 46 653
KpnI G_GTAC'C
Cuts at: 0 303 699
Size: 303 396
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 472 673 699
Size: 472 201 26
Fragments arranged by size:
472 201 26
MscI TGG'CCA
Cuts at: 0 209 511 699
Size: 209 302 188
Fragments arranged by size:
302 209 188
MslI CAynn'nnrTG
Cuts at: 0 655 699
Size: 655 44
MspA1I CmG'CkG
Cuts at: 0 68 190 635 699
Size: 68 122 445 64
Fragments arranged by size:
445 122 68 64
NaeI GCC'GGC
Cuts at: 0 663 699
Size: 663 36
NcoI C'CATG_G
Cuts at: 0 245 699
Size: 245 454
NgoMIV G'CCGG_C
Cuts at: 0 661 699
Size: 661 38
PasI CC'CwG_GG
Cuts at: 0 346 699
Size: 346 353
PfoI T'CCnGG_A
Cuts at: 0 392 545 699
Size: 392 153 154
Fragments arranged by size:
392 154 153
PvuII CAG'CTG
Cuts at: 0 68 699
Size: 68 631
SacII CC_GC'GG
Cuts at: 0 191 699
Size: 191 508
SmaI CCC'GGG
Cuts at: 0 232 699
Size: 232 467
StyI C'CwwG_G
Cuts at: 0 245 538 699
Size: 245 293 161
Fragments arranged by size:
293 245 161
XmaI C'CCGG_G
Cuts at: 0 230 699
Size: 230 469
Enzymes that do cut:
AccI Acc65I AcuI AfeI AvaI BaeI BamHI BanI
BanII BbvCI BciVI BglI Bme1580I BmrI BpmI Bpu10I
BsaI BsaXI BseYI BsmI Bsp1286I BspMI BsrFI BstYI
BtgI EaeI EarI Eco57MI EcoO109I HaeII Hin4I HincII
HindIII KpnI MmeI MscI MslI MspA1I NaeI NcoI
NgoMIV PasI PfoI PvuII SacII SmaI StyI XmaI
Enzymes that do not cut:
AarI AatII AclI AflII AflIII AgeI AhdI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BbeI BbsI BcgI BclI BfrBI BglII
BlpI BmgBI BmtI BplI BpuEI BsaAI BsaBI BsaHI
BsaWI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BspEI
BspHI BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EagI EciI EcoICRI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HpaI KasI MfeI
MluI NarI NdeI NheI NotI NruI NsiI NspI
PacI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI
SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmnI ZraI