Restriction map for
pF1KB8899
(Linear) (Six-base) MAPSORT of: KIBB8899.seq Check: 5425 from: 1 to: 699
KIBB8899.orf
With 182 enzymes: SgfI *
March 2, 2010 14:29 ..
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 502 534 699
Size: 502 32 165
Fragments arranged by size:
502 165 32
AlwNI CAG_nnn'CTG
Cuts at: 0 501 699
Size: 501 198
ApaI G_GGCC'C
Cuts at: 0 295 699
Size: 295 404
AvaI C'yCGr_G
Cuts at: 0 31 270 295 319 699
Size: 31 239 25 24 380
Fragments arranged by size:
380 239 31 25 24
BanI G'GyrC_C
Cuts at: 0 259 379 464 545 699
Size: 259 120 85 81 154
Fragments arranged by size:
259 154 120 85 81
BanII G_rGCy'C
Cuts at: 0 295 403 699
Size: 295 108 296
Fragments arranged by size:
296 295 108
BbeI G_GCGC'C
Cuts at: 0 263 383 468 549 699
Size: 263 120 85 81 150
Fragments arranged by size:
263 150 120 85 81
BbvCI CC'TCA_GC
Cuts at: 0 103 699
Size: 103 596
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 543 577 699
Size: 543 34 122
Fragments arranged by size:
543 122 34
BglII A'GATC_T
Cuts at: 0 599 699
Size: 599 100
Bme1580I G_kGCm'C
Cuts at: 0 295 699
Size: 295 404
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 523 529 699
Size: 523 6 170
Fragments arranged by size:
523 170 6
Bpu10I CC'TnA_GC
Cuts at: 0 103 699
Size: 103 596
BsaBI GATnn'nnATC
Cuts at: 0 598 699
Size: 598 101
BsaHI Gr'CG_yC
Cuts at: 0 130 208 260 380 465 546 699
Size: 130 78 52 120 85 81 153
Fragments arranged by size:
153 130 120 85 81 78 52
BsaWI w'CCGG_w
Cuts at: 0 386 699
Size: 386 313
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 502 532 699
Size: 502 30 167
Fragments arranged by size:
502 167 30
BseYI C'CCAG_C
Cuts at: 0 43 664 699
Size: 43 621 35
Fragments arranged by size:
621 43 35
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 292 699
Size: 292 407
BsiEI CG_ry'CG
Cuts at: 0 387 699
Size: 387 312
BsiWI C'GTAC_G
Cuts at: 0 122 699
Size: 122 577
Bsp1286I G_dGCh'C
Cuts at: 0 295 403 699
Size: 295 108 296
Fragments arranged by size:
296 295 108
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 314 699
Size: 314 385
BssSI C'ACGA_G
Cuts at: 0 228 396 699
Size: 228 168 303
Fragments arranged by size:
303 228 168
BstYI r'GATC_y
Cuts at: 0 599 699
Size: 599 100
BtgI C'CryG_G
Cuts at: 0 459 699
Size: 459 240
BtsI GCAGTG_nn'
Cuts at: 0 282 699
Size: 282 417
EaeI y'GGCC_r
Cuts at: 0 48 699
Size: 48 651
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 523 529 699
Size: 523 6 170
Fragments arranged by size:
523 170 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 82 699
Size: 82 617
EcoO109I rG'GnC_Cy
Cuts at: 0 107 224 249 291 292 699
Size: 107 117 25 42 1 407
Fragments arranged by size:
407 117 107 42 25 1
HaeII r_GCGC'y
Cuts at: 0 263 383 468 549 591 699
Size: 263 120 85 81 42 108
Fragments arranged by size:
263 120 108 85 81 42
KasI G'GCGC_C
Cuts at: 0 259 379 464 545 699
Size: 259 120 85 81 154
Fragments arranged by size:
259 154 120 85 81
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 206 699
Size: 206 493
MspA1I CmG'CkG
Cuts at: 0 47 72 350 423 668 699
Size: 47 25 278 73 245 31
Fragments arranged by size:
278 245 73 47 31 25
NarI GG'CG_CC
Cuts at: 0 260 380 465 546 699
Size: 260 120 85 81 153
Fragments arranged by size:
260 153 120 85 81
NcoI C'CATG_G
Cuts at: 0 459 699
Size: 459 240
PflMI CCAn_nnn'nTGG
Cuts at: 0 501 699
Size: 501 198
PfoI T'CCnGG_A
Cuts at: 0 140 699
Size: 140 559
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 502 534 699
Size: 502 32 165
Fragments arranged by size:
502 165 32
PpuMI rG'GwC_Cy
Cuts at: 0 249 699
Size: 249 450
PspOMI G'GGCC_C
Cuts at: 0 291 699
Size: 291 408
PvuII CAG'CTG
Cuts at: 0 72 699
Size: 72 627
SfoI GGC'GCC
Cuts at: 0 261 381 466 547 699
Size: 261 120 85 81 152
Fragments arranged by size:
261 152 120 85 81
SmaI CCC'GGG
Cuts at: 0 33 297 699
Size: 33 264 402
Fragments arranged by size:
402 264 33
StyI C'CwwG_G
Cuts at: 0 412 459 699
Size: 412 47 240
Fragments arranged by size:
412 240 47
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 177 699
Size: 177 522
TatI w'GTAC_w
Cuts at: 0 329 392 699
Size: 329 63 307
Fragments arranged by size:
329 307 63
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 189 699
Size: 189 510
XmaI C'CCGG_G
Cuts at: 0 31 295 699
Size: 31 264 404
Fragments arranged by size:
404 264 31
XmnI GAAnn'nnTTC
Cuts at: 0 517 699
Size: 517 182
Enzymes that do cut:
AloI AlwNI ApaI AvaI BanI BanII BbeI BbvCI
BcgI BglII Bme1580I BpmI Bpu10I BsaBI BsaHI BsaWI
BsaXI BseYI BsgI BsiEI BsiWI Bsp1286I BspMI BssSI
BstYI BtgI BtsI EaeI Eco57MI EcoNI EcoO109I HaeII
KasI MmeI MspA1I NarI NcoI PflMI PfoI PpiI
PpuMI PspOMI PvuII SfoI SmaI StyI TaqII TatI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbsI BciVI BclI
BfrBI BglI BlpI BmgBI BmrI BmtI BplI BpuEI
BsaI BsaAI BseRI BsiHKAI BsmI BsmBI BspEI BspHI
BsrBI BsrDI BsrFI BsrGI BssHII BstAPI BstBI BstEII
BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII
DrdI EagI EarI EciI EcoICRI EcoRI EcoRV FalI
FseI FspI FspAI Hin4I HincII HindIII HpaI KpnI
MfeI MluI MscI MslI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PmeI
PmlI PshAI PsiI PspXI PsrI PstI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfiI SgfI SgrAI SmlI SnaBI SpeI SphI
SrfI SspI StuI SwaI TstI Tth111I XbaI XhoI
ZraI