Restriction map for
pF1KB8897
(Linear) (Six-base) MAPSORT of: KIBB8897.seq Check: 100 from: 1 to: 672
KIBB8897.orf
With 182 enzymes: SgfI *
March 3, 2008 11:09 ..
AccI GT'mk_AC
Cuts at: 0 380 672
Size: 380 292
AflIII A'CryG_T
Cuts at: 0 460 672
Size: 460 212
AlwNI CAG_nnn'CTG
Cuts at: 0 474 672
Size: 474 198
ApaI G_GGCC'C
Cuts at: 0 431 672
Size: 431 241
ApoI r'AATT_y
Cuts at: 0 402 672
Size: 402 270
AvaI C'yCGr_G
Cuts at: 0 301 672
Size: 301 371
BanI G'GyrC_C
Cuts at: 0 207 228 274 672
Size: 207 21 46 398
Fragments arranged by size:
398 207 46 21
BanII G_rGCy'C
Cuts at: 0 431 672
Size: 431 241
BbeI G_GCGC'C
Cuts at: 0 211 232 278 672
Size: 211 21 46 394
Fragments arranged by size:
394 211 46 21
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 516 550 672
Size: 516 34 122
Fragments arranged by size:
516 122 34
BglI GCCn_nnn'nGGC
Cuts at: 0 129 443 672
Size: 129 314 229
Fragments arranged by size:
314 229 129
BlpI GC'TnA_GC
Cuts at: 0 622 672
Size: 622 50
Bme1580I G_kGCm'C
Cuts at: 0 431 672
Size: 431 241
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 496 502 672
Size: 496 6 170
Fragments arranged by size:
496 170 6
BsaHI Gr'CG_yC
Cuts at: 0 208 229 275 672
Size: 208 21 46 397
Fragments arranged by size:
397 208 46 21
BsaWI w'CCGG_w
Cuts at: 0 375 672
Size: 375 297
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 403 433 475 505 672
Size: 403 30 42 30 167
Fragments arranged by size:
403 167 42 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 661 664 672
Size: 661 3 8
Fragments arranged by size:
661 8 3
BseYI C'CCAG_C
Cuts at: 0 430 672
Size: 430 242
BsiEI CG_ry'CG
Cuts at: 0 201 438 672
Size: 201 237 234
Fragments arranged by size:
237 234 201
BsiHKAI G_wGCw'C
Cuts at: 0 370 672
Size: 370 302
BsmBI CGTCTCn'nnnn_
Cuts at: 0 635 672
Size: 635 37
Bsp1286I G_dGCh'C
Cuts at: 0 370 431 672
Size: 370 61 241
Fragments arranged by size:
370 241 61
BsrBI CCG'CTC
Cuts at: 0 78 205 672
Size: 78 127 467
Fragments arranged by size:
467 127 78
BsrFI r'CCGG_y
Cuts at: 0 180 225 231 437 672
Size: 180 45 6 206 235
Fragments arranged by size:
235 206 180 45 6
BssHII G'CGCG_C
Cuts at: 0 323 672
Size: 323 349
BtgI C'CryG_G
Cuts at: 0 307 385 672
Size: 307 78 287
Fragments arranged by size:
307 287 78
EaeI y'GGCC_r
Cuts at: 0 41 198 435 672
Size: 41 157 237 237
Fragments arranged by size:
237 237 157 41
EagI C'GGCC_G
Cuts at: 0 198 435 672
Size: 198 237 237
Fragments arranged by size:
237 237 198
EarI CTCTTCn'nnn_
Cuts at: 0 350 672
Size: 350 322
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 496 502 672
Size: 496 6 170
Fragments arranged by size:
496 170 6
EcoRI G'AATT_C
Cuts at: 0 402 672
Size: 402 270
FspI TGC'GCA
Cuts at: 0 264 672
Size: 264 408
HaeII r_GCGC'y
Cuts at: 0 211 232 278 672
Size: 211 21 46 394
Fragments arranged by size:
394 211 46 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 238 270 672
Size: 238 32 402
Fragments arranged by size:
402 238 32
KasI G'GCGC_C
Cuts at: 0 207 228 274 672
Size: 207 21 46 398
Fragments arranged by size:
398 207 46 21
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 296 672
Size: 296 376
MscI TGG'CCA
Cuts at: 0 43 672
Size: 43 629
MspA1I CmG'CkG
Cuts at: 0 47 105 309 396 434 672
Size: 47 58 204 87 38 238
Fragments arranged by size:
238 204 87 58 47 38
NaeI GCC'GGC
Cuts at: 0 182 227 233 439 672
Size: 182 45 6 206 233
Fragments arranged by size:
233 206 182 45 6
NarI GG'CG_CC
Cuts at: 0 208 229 275 672
Size: 208 21 46 397
Fragments arranged by size:
397 208 46 21
NgoMIV G'CCGG_C
Cuts at: 0 180 225 231 437 672
Size: 180 45 6 206 235
Fragments arranged by size:
235 206 180 45 6
PflMI CCAn_nnn'nTGG
Cuts at: 0 474 672
Size: 474 198
PspOMI G'GGCC_C
Cuts at: 0 427 672
Size: 427 245
SacII CC_GC'GG
Cuts at: 0 310 672
Size: 310 362
SapI GCTCTTCn'nnn_
Cuts at: 0 350 672
Size: 350 322
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 443 672
Size: 443 229
SfoI GGC'GCC
Cuts at: 0 209 230 276 672
Size: 209 21 46 396
Fragments arranged by size:
396 209 46 21
SgrAI Cr'CCGG_yG
Cuts at: 0 180 672
Size: 180 492
Enzymes that do cut:
AccI AflIII AlwNI ApaI ApoI AvaI BanI BanII
BbeI BcgI BglI BlpI Bme1580I BpmI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BsrBI
BsrFI BssHII BtgI EaeI EagI EarI Eco57MI EcoRI
FspI HaeII Hin4I KasI MmeI MscI MspA1I NaeI
NarI NgoMIV PflMI PspOMI SacII SapI SfiI SfoI
SgrAI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BbsI BbvCI BciVI BclI BfrBI
BglII BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaI
BsaAI BsaBI BsgI BsiWI BsmI BspEI BspHI BspMI
BsrDI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EciI EcoICRI EcoNI EcoO109I EcoRV FalI FseI
FspAI HincII HindIII HpaI KpnI MfeI MluI MslI
NcoI NdeI NheI NotI NruI NsiI NspI PacI
PasI PciI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PsrI PstI PvuI PvuII RsrII SacI
SalI SanDI SbfI ScaI SexAI SfcI SgfI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI