Restriction map for
pF1KB8894
(Linear) (Six-base) MAPSORT of: KIBB8894.seq Check: 6309 from: 1 to: 651
KIBB8894.orf
With 182 enzymes: SgfI *
March 3, 2008 11:09 ..
AlwNI CAG_nnn'CTG
Cuts at: 0 101 651
Size: 101 550
ApaI G_GGCC'C
Cuts at: 0 266 636 651
Size: 266 370 15
Fragments arranged by size:
370 266 15
ApaLI G'TGCA_C
Cuts at: 0 37 651
Size: 37 614
AvaI C'yCGr_G
Cuts at: 0 151 196 267 651
Size: 151 45 71 384
Fragments arranged by size:
384 151 71 45
BanI G'GyrC_C
Cuts at: 0 211 246 256 304 651
Size: 211 35 10 48 347
Fragments arranged by size:
347 211 48 35 10
BanII G_rGCy'C
Cuts at: 0 266 636 644 651
Size: 266 370 8 7
Fragments arranged by size:
370 266 8 7
BbeI G_GCGC'C
Cuts at: 0 215 260 651
Size: 215 45 391
Fragments arranged by size:
391 215 45
BbsI GAAGACnn'nnnn_
Cuts at: 0 523 651
Size: 523 128
BglI GCCn_nnn'nGGC
Cuts at: 0 502 611 651
Size: 502 109 40
Fragments arranged by size:
502 109 40
Bme1580I G_kGCm'C
Cuts at: 0 41 266 636 651
Size: 41 225 370 15
Fragments arranged by size:
370 225 41 15
BmgBI CAC'GTC
Cuts at: 0 525 627 651
Size: 525 102 24
Fragments arranged by size:
525 102 24
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 628 651
Size: 628 23
BsaHI Gr'CG_yC
Cuts at: 0 212 257 651
Size: 212 45 394
Fragments arranged by size:
394 212 45
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 127 547 651
Size: 127 420 104
Fragments arranged by size:
420 127 104
BsiHKAI G_wGCw'C
Cuts at: 0 41 644 651
Size: 41 603 7
Fragments arranged by size:
603 41 7
Bsp1286I G_dGCh'C
Cuts at: 0 41 266 636 644 651
Size: 41 225 370 8 7
Fragments arranged by size:
370 225 41 8 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 467 651
Size: 467 184
BsrBI CCG'CTC
Cuts at: 0 324 651
Size: 324 327
BsrFI r'CCGG_y
Cuts at: 0 217 651
Size: 217 434
BssSI C'ACGA_G
Cuts at: 0 43 651
Size: 43 608
BtgI C'CryG_G
Cuts at: 0 129 651
Size: 129 522
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 426 651
Size: 426 225
DraIII CAC_nnn'GTG
Cuts at: 0 33 651
Size: 33 618
EaeI y'GGCC_r
Cuts at: 0 144 428 464 616 651
Size: 144 284 36 152 35
Fragments arranged by size:
284 152 144 36 35
EarI CTCTTCn'nnn_
Cuts at: 0 532 651
Size: 532 119
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 505 651
Size: 505 146
EcoICRI GAG'CTC
Cuts at: 0 642 651
Size: 642 9
EcoO109I rG'GnC_Cy
Cuts at: 0 263 633 651
Size: 263 370 18
Fragments arranged by size:
370 263 18
HaeII r_GCGC'y
Cuts at: 0 215 260 300 353 651
Size: 215 45 40 53 298
Fragments arranged by size:
298 215 53 45 40
KasI G'GCGC_C
Cuts at: 0 211 256 651
Size: 211 45 395
Fragments arranged by size:
395 211 45
MscI TGG'CCA
Cuts at: 0 430 466 651
Size: 430 36 185
Fragments arranged by size:
430 185 36
MspA1I CmG'CkG
Cuts at: 0 79 101 209 651
Size: 79 22 108 442
Fragments arranged by size:
442 108 79 22
NaeI GCC'GGC
Cuts at: 0 219 651
Size: 219 432
NarI GG'CG_CC
Cuts at: 0 212 257 651
Size: 212 45 394
Fragments arranged by size:
394 212 45
NcoI C'CATG_G
Cuts at: 0 129 651
Size: 129 522
NgoMIV G'CCGG_C
Cuts at: 0 217 651
Size: 217 434
PflMI CCAn_nnn'nTGG
Cuts at: 0 33 651
Size: 33 618
PspOMI G'GGCC_C
Cuts at: 0 262 632 651
Size: 262 370 19
Fragments arranged by size:
370 262 19
PstI C_TGCA'G
Cuts at: 0 106 651
Size: 106 545
PvuII CAG'CTG
Cuts at: 0 79 651
Size: 79 572
SacI G_AGCT'C
Cuts at: 0 644 651
Size: 644 7
SfcI C'TryA_G
Cuts at: 0 102 171 189 651
Size: 102 69 18 462
Fragments arranged by size:
462 102 69 18
SfoI GGC'GCC
Cuts at: 0 213 258 651
Size: 213 45 393
Fragments arranged by size:
393 213 45
SmaI CCC'GGG
Cuts at: 0 269 651
Size: 269 382
SmlI C'TyrA_G
Cuts at: 0 643 651
Size: 643 8
StuI AGG'CCT
Cuts at: 0 496 651
Size: 496 155
StyI C'CwwG_G
Cuts at: 0 129 467 651
Size: 129 338 184
Fragments arranged by size:
338 184 129
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 535 651
Size: 535 116
XmaI C'CCGG_G
Cuts at: 0 267 651
Size: 267 384
Enzymes that do cut:
AlwNI ApaI ApaLI AvaI BanI BanII BbeI BbsI
BglI Bme1580I BmgBI BpuEI BsaHI BseRI BsiHKAI Bsp1286I
BspMI BsrBI BsrFI BssSI BtgI BtgZI DraIII EaeI
EarI EciI EcoICRI EcoO109I HaeII KasI MscI MspA1I
NaeI NarI NcoI NgoMIV PflMI PspOMI PstI PvuII
SacI SfcI SfoI SmaI SmlI StuI StyI TaqII
XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI
BclI BfrBI BglII BlpI BmrI BmtI BplI BpmI
Bpu10I BsaI BsaAI BsaBI BsaWI BsaXI BseYI BsgI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrGI
BssHII BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtsI ClaI CspCI DraI DrdI EagI Eco57MI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MslI NdeI
NheI NotI NruI NsiI NspI PacI PasI PciI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI
PsrI PvuI RsrII SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI SwaI TatI TstI Tth111I XbaI XcmI
XhoI XmnI ZraI