Restriction map for
pF1KB8892
(Linear) (Six-base) MAPSORT of: KIBB8892.seq Check: 3131 from: 1 to: 645
KIBB8892.orf
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
ApaI G_GGCC'C
Cuts at: 0 577 645
Size: 577 68
AvaI C'yCGr_G
Cuts at: 0 244 475 645
Size: 244 231 170
Fragments arranged by size:
244 231 170
BanII G_rGCy'C
Cuts at: 0 145 244 577 645
Size: 145 99 333 68
Fragments arranged by size:
333 145 99 68
BglI GCCn_nnn'nGGC
Cuts at: 0 275 487 645
Size: 275 212 158
Fragments arranged by size:
275 212 158
BlpI GC'TnA_GC
Cuts at: 0 139 645
Size: 139 506
Bme1580I G_kGCm'C
Cuts at: 0 577 645
Size: 577 68
BmgBI CAC'GTC
Cuts at: 0 444 645
Size: 444 201
BmrI ACTGGGnnnn_n'
Cuts at: 0 571 645
Size: 571 74
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 274 645
Size: 274 371
Bpu10I CC'TnA_GC
Cuts at: 0 291 645
Size: 291 354
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 481 645
Size: 481 164
BsaHI Gr'CG_yC
Cuts at: 0 224 645
Size: 224 421
BsiEI CG_ry'CG
Cuts at: 0 194 270 377 516 645
Size: 194 76 107 139 129
Fragments arranged by size:
194 139 129 107 76
BsmI GAATG_Cn'
Cuts at: 0 337 645
Size: 337 308
BsmBI CGTCTCn'nnnn_
Cuts at: 0 306 645
Size: 306 339
Bsp1286I G_dGCh'C
Cuts at: 0 145 244 577 645
Size: 145 99 333 68
Fragments arranged by size:
333 145 99 68
BsrBI CCG'CTC
Cuts at: 0 309 645
Size: 309 336
BsrFI r'CCGG_y
Cuts at: 0 278 372 604 645
Size: 278 94 232 41
Fragments arranged by size:
278 232 94 41
BssSI C'ACGA_G
Cuts at: 0 7 645
Size: 7 638
BstAPI GCAn_nnn'nTGC
Cuts at: 0 63 645
Size: 63 582
BtgI C'CryG_G
Cuts at: 0 188 645
Size: 188 457
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 411 645
Size: 411 234
EaeI y'GGCC_r
Cuts at: 0 191 267 276 374 449 513 610 645
Size: 191 76 9 98 75 64 97 35
Fragments arranged by size:
191 98 97 76 75 64 35 9
EagI C'GGCC_G
Cuts at: 0 191 267 374 513 645
Size: 191 76 107 139 132
Fragments arranged by size:
191 139 132 107 76
EarI CTCTTCn'nnn_
Cuts at: 0 88 580 645
Size: 88 492 65
Fragments arranged by size:
492 88 65
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 297 520 645
Size: 297 223 125
Fragments arranged by size:
297 223 125
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 274 645
Size: 274 371
EcoO109I rG'GnC_Cy
Cuts at: 0 116 295 645
Size: 116 179 350
Fragments arranged by size:
350 179 116
FspI TGC'GCA
Cuts at: 0 56 645
Size: 56 589
HaeII r_GCGC'y
Cuts at: 0 262 631 645
Size: 262 369 14
Fragments arranged by size:
369 262 14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 387 419 448 480 645
Size: 387 32 29 32 165
Fragments arranged by size:
387 165 32 32 29
MscI TGG'CCA
Cuts at: 0 451 645
Size: 451 194
MspA1I CmG'CkG
Cuts at: 0 190 196 645
Size: 190 6 449
Fragments arranged by size:
449 190 6
NaeI GCC'GGC
Cuts at: 0 280 374 645
Size: 280 94 271
Fragments arranged by size:
280 271 94
NgoMIV G'CCGG_C
Cuts at: 0 278 372 645
Size: 278 94 273
Fragments arranged by size:
278 273 94
NotI GC'GGCC_GC
Cuts at: 0 191 645
Size: 191 454
PasI CC'CwG_GG
Cuts at: 0 570 645
Size: 570 75
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 65 97 645
Size: 65 32 548
Fragments arranged by size:
548 65 32
PpuMI rG'GwC_Cy
Cuts at: 0 295 645
Size: 295 350
PspOMI G'GGCC_C
Cuts at: 0 573 645
Size: 573 72
PstI C_TGCA'G
Cuts at: 0 201 545 645
Size: 201 344 100
Fragments arranged by size:
344 201 100
RsrII CG'GwC_CG
Cuts at: 0 88 645
Size: 88 557
SacII CC_GC'GG
Cuts at: 0 191 645
Size: 191 454
SfcI C'TryA_G
Cuts at: 0 197 541 645
Size: 197 344 104
Fragments arranged by size:
344 197 104
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 275 645
Size: 275 370
SmaI CCC'GGG
Cuts at: 0 246 645
Size: 246 399
SmlI C'TyrA_G
Cuts at: 0 496 645
Size: 496 149
StuI AGG'CCT
Cuts at: 0 231 481 645
Size: 231 250 164
Fragments arranged by size:
250 231 164
StyI C'CwwG_G
Cuts at: 0 452 645
Size: 452 193
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 76 645
Size: 76 569
XmaI C'CCGG_G
Cuts at: 0 244 645
Size: 244 401
XmnI GAAnn'nnTTC
Cuts at: 0 557 645
Size: 557 88
Enzymes that do cut:
ApaI AvaI BanII BglI BlpI Bme1580I BmgBI BmrI
BpmI Bpu10I BpuEI BsaHI BsiEI BsmI BsmBI Bsp1286I
BsrBI BsrFI BssSI BstAPI BtgI BtgZI EaeI EagI
EarI EciI Eco57MI EcoO109I FspI HaeII Hin4I MscI
MspA1I NaeI NgoMIV NotI PasI PpiI PpuMI PspOMI
PstI RsrII SacII SfcI SfiI SmaI SmlI StuI
StyI TaqII XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BanI
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglII
BmtI BplI BsaI BsaAI BsaBI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsiWI BspEI BspHI BspMI BsrDI
BsrGI BssHII BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtsI ClaI CspCI DraI DraIII DrdI EcoICRI EcoNI
EcoRI EcoRV FalI FseI FspAI HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MslI NarI NcoI
NdeI NheI NruI NsiI NspI PacI PciI PflMI
PfoI PmeI PmlI PshAI PsiI PspXI PsrI PvuI
PvuII SacI SalI SanDI SapI SbfI ScaI SexAI
SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
SwaI TatI TstI Tth111I XbaI XcmI XhoI ZraI