(Linear) (Six-base) MAPSORT of: KIBB8890.seq  Check: 8614  from: 1  to: 624



KIBB8890.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:09  ..

AflII C'TTAA_G

Cuts at:      0     178     624 
   Size:        178     446
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     472     624 
   Size:        472     152
 
ApaI G_GGCC'C

Cuts at:      0     216     624 
   Size:        216     408
 
BanI G'GyrC_C

Cuts at:      0       6      40     240     456     624 
   Size:          6      34     200     216     168

  Fragments arranged by size: 

                 216     200     168      34       6
 
BanII G_rGCy'C

Cuts at:      0     216     624 
   Size:        216     408
 
Bme1580I G_kGCm'C

Cuts at:      0      43     216     243     624 
   Size:         43     173      27     381

  Fragments arranged by size: 

                 381     173      43      27
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      44     624 
   Size:         44     580
 
Bpu10I CC'TnA_GC

Cuts at:      0     392     624 
   Size:        392     232
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     549     624 
   Size:        549      75
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      49      79     624 
   Size:         49      30     545

  Fragments arranged by size: 

                 545      49      30
 
BseYI C'CCAG_C

Cuts at:      0     529     624 
   Size:        529      95
 
Bsp1286I G_dGCh'C

Cuts at:      0      43     216     243     624 
   Size:         43     173      27     381

  Fragments arranged by size: 

                 381     173      43      27
 
BspHI T'CATG_A

Cuts at:      0     419     624 
   Size:        419     205
 
BsrDI GCAATG_nn'

Cuts at:      0     320     624 
   Size:        320     304
 
BsrGI T'GTAC_A

Cuts at:      0     434     624 
   Size:        434     190
 
BssSI C'ACGA_G

Cuts at:      0     502     624 
   Size:        502     122
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      67     178     624 
   Size:         67     111     446

  Fragments arranged by size: 

                 446     111      67
 
EcoO109I rG'GnC_Cy

Cuts at:      0     212     624 
   Size:        212     412
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     472     504     529     561     624 
   Size:        472      32      25      32      63

  Fragments arranged by size: 

                 472      63      32      32      25
 
MslI CAynn'nnrTG

Cuts at:      0      22      49     454     624 
   Size:         22      27     405     170

  Fragments arranged by size: 

                 405     170      27      22
 
MspA1I CmG'CkG

Cuts at:      0     529     624 
   Size:        529      95
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     165     300     624 
   Size:        165     135     324

  Fragments arranged by size: 

                 324     165     135
 
PspOMI G'GGCC_C

Cuts at:      0     212     624 
   Size:        212     412
 
SmlI C'TyrA_G

Cuts at:      0     178     624 
   Size:        178     446
 
StuI AGG'CCT

Cuts at:      0     484     624 
   Size:        484     140
 
TatI w'GTAC_w

Cuts at:      0     434     624 
   Size:        434     190
 
Tth111I GACn'n_nGTC

Cuts at:      0     541     624 
   Size:        541      83
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      51     115     624 
   Size:         51      64     509

  Fragments arranged by size: 

                 509      64      51
 
XmnI GAAnn'nnTTC

Cuts at:      0     562     624 
   Size:        562      62
 

 Enzymes that do cut:

    AflII     AlfI     ApaI     BanI    BanII Bme1580I     BmrI   Bpu10I
     BsaI    BsaXI    BseYI Bsp1286I    BspHI    BsrDI    BsrGI    BssSI
    EcoNI EcoO109I    Hin4I     MslI   MspA1I    PflMI   PspOMI     SmlI
     StuI     TatI  Tth111I     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI   AflIII
     AgeI     AhdI     AleI     AloI    AlwNI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
    BmgBI     BmtI     BplI     BpmI    BpuEI    BsaAI    BsaBI    BsaHI
    BsaWI    BseRI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
    BspEI    BspMI    BsrBI    BsrFI   BssHII   BstAPI    BstBI   BstEII
    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StyI
     SwaI    TaqII     TstI     XbaI     XhoI     XmaI     ZraI