Restriction map for
pF1KB8885
(Linear) (Six-base) MAPSORT of: KIBB8885.seq Check: 7810 from: 1 to: 579
KIBB8885.orf
With 182 enzymes: SgfI *
March 3, 2008 11:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 57 579
Size: 57 522
ApaI G_GGCC'C
Cuts at: 0 384 579
Size: 384 195
AvaI C'yCGr_G
Cuts at: 0 192 343 579
Size: 192 151 236
Fragments arranged by size:
236 192 151
BanI G'GyrC_C
Cuts at: 0 40 421 579
Size: 40 381 158
Fragments arranged by size:
381 158 40
BanII G_rGCy'C
Cuts at: 0 82 180 384 433 579
Size: 82 98 204 49 146
Fragments arranged by size:
204 146 98 82 49
BglI GCCn_nnn'nGGC
Cuts at: 0 175 579
Size: 175 404
Bme1580I G_kGCm'C
Cuts at: 0 384 426 579
Size: 384 42 153
Fragments arranged by size:
384 153 42
BmrI ACTGGGnnnn_n'
Cuts at: 0 378 448 502 579
Size: 378 70 54 77
Fragments arranged by size:
378 77 70 54
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 96 579
Size: 96 483
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 317 579
Size: 317 262
BsaWI w'CCGG_w
Cuts at: 0 519 579
Size: 519 60
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 210 240 579
Size: 210 30 339
Fragments arranged by size:
339 210 30
BseYI C'CCAG_C
Cuts at: 0 531 579
Size: 531 48
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 5 118 238 579
Size: 5 113 120 341
Fragments arranged by size:
341 120 113 5
BsiHKAI G_wGCw'C
Cuts at: 0 82 579
Size: 82 497
Bsp1286I G_dGCh'C
Cuts at: 0 82 180 384 426 433 579
Size: 82 98 204 42 7 146
Fragments arranged by size:
204 146 98 82 42 7
BspEI T'CCGG_A
Cuts at: 0 519 579
Size: 519 60
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 57 260 579
Size: 57 203 319
Fragments arranged by size:
319 203 57
BstYI r'GATC_y
Cuts at: 0 196 579
Size: 196 383
BtgI C'CryG_G
Cuts at: 0 83 579
Size: 83 496
BtsI GCAGTG_nn'
Cuts at: 0 128 554 579
Size: 128 426 25
Fragments arranged by size:
426 128 25
ClaI AT'CG_AT
Cuts at: 0 443 579
Size: 443 136
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 153 188 579
Size: 153 35 391
Fragments arranged by size:
391 153 35
EaeI y'GGCC_r
Cuts at: 0 125 579
Size: 125 454
EcoICRI GAG'CTC
Cuts at: 0 80 579
Size: 80 499
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 317 579
Size: 317 262
EcoO109I rG'GnC_Cy
Cuts at: 0 355 380 579
Size: 355 25 199
Fragments arranged by size:
355 199 25
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 553 579
Size: 553 26
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 119 479 579
Size: 119 360 100
Fragments arranged by size:
360 119 100
MscI TGG'CCA
Cuts at: 0 127 579
Size: 127 452
MspA1I CmG'CkG
Cuts at: 0 288 579
Size: 288 291
PasI CC'CwG_GG
Cuts at: 0 142 579
Size: 142 437
PpuMI rG'GwC_Cy
Cuts at: 0 355 579
Size: 355 224
PspOMI G'GGCC_C
Cuts at: 0 380 579
Size: 380 199
PstI C_TGCA'G
Cuts at: 0 293 579
Size: 293 286
PvuII CAG'CTG
Cuts at: 0 288 579
Size: 288 291
SacI G_AGCT'C
Cuts at: 0 82 579
Size: 82 497
SfcI C'TryA_G
Cuts at: 0 289 579
Size: 289 290
SmaI CCC'GGG
Cuts at: 0 345 579
Size: 345 234
SpeI A'CTAG_T
Cuts at: 0 184 579
Size: 184 395
StuI AGG'CCT
Cuts at: 0 18 579
Size: 18 561
StyI C'CwwG_G
Cuts at: 0 164 579
Size: 164 415
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 518 579
Size: 518 61
XmaI C'CCGG_G
Cuts at: 0 343 579
Size: 343 236
Enzymes that do cut:
AarI ApaI AvaI BanI BanII BglI Bme1580I BmrI
BplI BpmI BsaWI BsaXI BseYI BsgI BsiHKAI Bsp1286I
BspEI BspMI BstYI BtgI BtsI ClaI CspCI EaeI
EcoICRI Eco57MI EcoO109I Hin4I MmeI MscI MspA1I PasI
PpuMI PspOMI PstI PvuII SacI SfcI SmaI SpeI
StuI StyI TaqII XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglII BlpI BmgBI
BmtI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BseRI
BsiEI BsiWI BsmI BsmBI BspHI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I
Bsu36I BtgZI DraI DraIII DrdI EagI EarI EciI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HaeII
HincII HindIII HpaI KasI KpnI MfeI MluI MslI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PciI PflMI PfoI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmlI SnaBI SphI SrfI SspI SwaI
TatI TstI Tth111I XbaI XcmI XhoI XmnI ZraI