Restriction map for
pF1KB8881
(Linear) (Six-base) MAPSORT of: KIBB8881.seq Check: 5110 from: 1 to: 537
KIBB8881.orf
With 182 enzymes: SgfI *
March 3, 2008 11:09 ..
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 295 537
Size: 295 242
AlwNI CAG_nnn'CTG
Cuts at: 0 402 537
Size: 402 135
ApaI G_GGCC'C
Cuts at: 0 270 537
Size: 270 267
ApoI r'AATT_y
Cuts at: 0 88 537
Size: 88 449
AvaI C'yCGr_G
Cuts at: 0 478 537
Size: 478 59
BanI G'GyrC_C
Cuts at: 0 183 324 537
Size: 183 141 213
Fragments arranged by size:
213 183 141
BanII G_rGCy'C
Cuts at: 0 124 202 270 537
Size: 124 78 68 267
Fragments arranged by size:
267 124 78 68
BbeI G_GCGC'C
Cuts at: 0 187 537
Size: 187 350
BbvCI CC'TCA_GC
Cuts at: 0 16 537
Size: 16 521
BglI GCCn_nnn'nGGC
Cuts at: 0 161 537
Size: 161 376
BlpI GC'TnA_GC
Cuts at: 0 283 537
Size: 283 254
Bme1580I G_kGCm'C
Cuts at: 0 270 329 537
Size: 270 59 208
Fragments arranged by size:
270 208 59
BmgBI CAC'GTC
Cuts at: 0 452 537
Size: 452 85
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 138 537
Size: 138 399
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 282 537
Size: 282 255
Bpu10I CC'TnA_GC
Cuts at: 0 16 205 361 537
Size: 16 189 156 176
Fragments arranged by size:
189 176 156 16
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 289 411 537
Size: 289 122 126
Fragments arranged by size:
289 126 122
BsaI GGTCTCn'nnnn_
Cuts at: 0 146 537
Size: 146 391
BsaHI Gr'CG_yC
Cuts at: 0 184 370 537
Size: 184 186 167
Fragments arranged by size:
186 184 167
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 130 189 537
Size: 130 59 348
Fragments arranged by size:
348 130 59
BsiHKAI G_wGCw'C
Cuts at: 0 112 124 537
Size: 112 12 413
Fragments arranged by size:
413 112 12
Bsp1286I G_dGCh'C
Cuts at: 0 112 124 202 270 329 537
Size: 112 12 78 68 59 208
Fragments arranged by size:
208 112 78 68 59 12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 222 537
Size: 222 315
BsrBI CCG'CTC
Cuts at: 0 209 264 537
Size: 209 55 273
Fragments arranged by size:
273 209 55
BsrFI r'CCGG_y
Cuts at: 0 7 537
Size: 7 530
BstBI TT'CG_AA
Cuts at: 0 79 537
Size: 79 458
BstEII G'GTnAC_C
Cuts at: 0 501 537
Size: 501 36
BstYI r'GATC_y
Cuts at: 0 398 537
Size: 398 139
BtgI C'CryG_G
Cuts at: 0 156 537
Size: 156 381
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 35 366 537
Size: 35 331 171
Fragments arranged by size:
331 171 35
EaeI y'GGCC_r
Cuts at: 0 467 537
Size: 467 70
EarI CTCTTCn'nnn_
Cuts at: 0 190 537
Size: 190 347
EcoICRI GAG'CTC
Cuts at: 0 122 537
Size: 122 415
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 282 537
Size: 282 255
EcoNI CCTnn'n_nnAGG
Cuts at: 0 37 537
Size: 37 500
EcoO109I rG'GnC_Cy
Cuts at: 0 30 537
Size: 30 507
HaeII r_GCGC'y
Cuts at: 0 187 537
Size: 187 350
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 124 156 537
Size: 124 32 381
Fragments arranged by size:
381 124 32
HincII GTy'rAC
Cuts at: 0 444 537
Size: 444 93
KasI G'GCGC_C
Cuts at: 0 183 537
Size: 183 354
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 32 125 149 537
Size: 32 93 24 388
Fragments arranged by size:
388 93 32 24
MslI CAynn'nnrTG
Cuts at: 0 277 537
Size: 277 260
MspA1I CmG'CkG
Cuts at: 0 158 537
Size: 158 379
NarI GG'CG_CC
Cuts at: 0 184 537
Size: 184 353
NspI r_CATG'y
Cuts at: 0 276 537
Size: 276 261
PasI CC'CwG_GG
Cuts at: 0 320 537
Size: 320 217
PpuMI rG'GwC_Cy
Cuts at: 0 30 537
Size: 30 507
PspOMI G'GGCC_C
Cuts at: 0 266 537
Size: 266 271
SacI G_AGCT'C
Cuts at: 0 124 537
Size: 124 413
SacII CC_GC'GG
Cuts at: 0 159 537
Size: 159 378
SapI GCTCTTCn'nnn_
Cuts at: 0 190 537
Size: 190 347
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 161 537
Size: 161 376
SfoI GGC'GCC
Cuts at: 0 185 537
Size: 185 352
SmlI C'TyrA_G
Cuts at: 0 304 390 537
Size: 304 86 147
Fragments arranged by size:
304 147 86
SphI G_CATG'C
Cuts at: 0 276 537
Size: 276 261
StuI AGG'CCT
Cuts at: 0 292 537
Size: 292 245
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 312 537
Size: 312 225
Enzymes that do cut:
AlfI AlwNI ApaI ApoI AvaI BanI BanII BbeI
BbvCI BglI BlpI Bme1580I BmgBI BplI BpmI Bpu10I
BpuEI BsaI BsaHI BseRI BsiHKAI Bsp1286I BspMI BsrBI
BsrFI BstBI BstEII BstYI BtgI BtgZI EaeI EarI
EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII KasI
MmeI MslI MspA1I NarI NspI PasI PpuMI PspOMI
SacI SacII SapI SfiI SfoI SmlI SphI StuI
TaqII
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AloI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BbsI BcgI BciVI BclI
BfrBI BglII BmrI BmtI BsaAI BsaBI BsaWI BsaXI
BseYI BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI
BsrDI BsrGI BssHII BssSI BstAPI BstXI BstZ17I Bsu36I
BtsI ClaI CspCI DraI DraIII DrdI EagI EciI
EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI
KpnI MfeI MluI MscI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PstI
PvuI PvuII RsrII SalI SanDI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI
StyI SwaI TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI