Restriction map for
pF1KB8879
(Linear) (Six-base) MAPSORT of: KIBB8879.seq Check: 9461 from: 1 to: 519
KIBB8879.orf
With 182 enzymes: SgfI *
March 3, 2008 11:09 ..
AleI CACnn'nnGTG
Cuts at: 0 446 519
Size: 446 73
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 435 519
Size: 435 84
AlwNI CAG_nnn'CTG
Cuts at: 0 91 519
Size: 91 428
ApaLI G'TGCA_C
Cuts at: 0 410 519
Size: 410 109
AvaI C'yCGr_G
Cuts at: 0 336 423 519
Size: 336 87 96
Fragments arranged by size:
336 96 87
BanI G'GyrC_C
Cuts at: 0 69 237 519
Size: 69 168 282
Fragments arranged by size:
282 168 69
BanII G_rGCy'C
Cuts at: 0 101 375 519
Size: 101 274 144
Fragments arranged by size:
274 144 101
BbeI G_GCGC'C
Cuts at: 0 241 519
Size: 241 278
BbsI GAAGACnn'nnnn_
Cuts at: 0 315 519
Size: 315 204
BglI GCCn_nnn'nGGC
Cuts at: 0 110 250 341 519
Size: 110 140 91 178
Fragments arranged by size:
178 140 110 91
Bme1580I G_kGCm'C
Cuts at: 0 414 519
Size: 414 105
BmgBI CAC'GTC
Cuts at: 0 481 519
Size: 481 38
BmrI ACTGGGnnnn_n'
Cuts at: 0 20 519
Size: 20 499
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 128 519
Size: 128 391
BsaHI Gr'CG_yC
Cuts at: 0 238 519
Size: 238 281
BsaWI w'CCGG_w
Cuts at: 0 149 519
Size: 149 370
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 267 297 519
Size: 267 30 222
Fragments arranged by size:
267 222 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 288 519
Size: 288 231
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 440 519
Size: 440 79
BsiHKAI G_wGCw'C
Cuts at: 0 101 414 519
Size: 101 313 105
Fragments arranged by size:
313 105 101
Bsp1286I G_dGCh'C
Cuts at: 0 101 375 414 519
Size: 101 274 39 105
Fragments arranged by size:
274 105 101 39
BspEI T'CCGG_A
Cuts at: 0 149 519
Size: 149 370
BspHI T'CATG_A
Cuts at: 0 318 519
Size: 318 201
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 122 519
Size: 122 397
BssHII G'CGCG_C
Cuts at: 0 159 519
Size: 159 360
BssSI C'ACGA_G
Cuts at: 0 256 519
Size: 256 263
BstAPI GCAn_nnn'nTGC
Cuts at: 0 91 519
Size: 91 428
BstEII G'GTnAC_C
Cuts at: 0 141 519
Size: 141 378
BtgI C'CryG_G
Cuts at: 0 205 361 507 519
Size: 205 156 146 12
Fragments arranged by size:
205 156 146 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 501 519
Size: 501 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 359 519
Size: 359 160
EcoICRI GAG'CTC
Cuts at: 0 99 519
Size: 99 420
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 128 519
Size: 128 391
EcoO109I rG'GnC_Cy
Cuts at: 0 22 519
Size: 22 497
FspI TGC'GCA
Cuts at: 0 130 519
Size: 130 389
HaeII r_GCGC'y
Cuts at: 0 57 232 241 336 519
Size: 57 175 9 95 183
Fragments arranged by size:
183 175 95 57 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 267 299 519
Size: 267 32 220
Fragments arranged by size:
267 220 32
KasI G'GCGC_C
Cuts at: 0 237 519
Size: 237 282
MslI CAynn'nnrTG
Cuts at: 0 323 446 519
Size: 323 123 73
Fragments arranged by size:
323 123 73
MspA1I CmG'CkG
Cuts at: 0 88 207 247 519
Size: 88 119 40 272
Fragments arranged by size:
272 119 88 40
NarI GG'CG_CC
Cuts at: 0 238 519
Size: 238 281
PfoI T'CCnGG_A
Cuts at: 0 435 519
Size: 435 84
PpuMI rG'GwC_Cy
Cuts at: 0 22 519
Size: 22 497
PstI C_TGCA'G
Cuts at: 0 35 519
Size: 35 484
SacI G_AGCT'C
Cuts at: 0 101 519
Size: 101 418
SacII CC_GC'GG
Cuts at: 0 208 519
Size: 208 311
SanDI GG'GwC_CC
Cuts at: 0 22 519
Size: 22 497
SexAI A'CCwGG_T
Cuts at: 0 312 519
Size: 312 207
SfcI C'TryA_G
Cuts at: 0 31 519
Size: 31 488
SfoI GGC'GCC
Cuts at: 0 239 519
Size: 239 280
SmaI CCC'GGG
Cuts at: 0 425 519
Size: 425 94
Tth111I GACn'n_nGTC
Cuts at: 0 314 519
Size: 314 205
XmaI C'CCGG_G
Cuts at: 0 423 519
Size: 423 96
Enzymes that do cut:
AleI AlfI AlwNI ApaLI AvaI BanI BanII BbeI
BbsI BglI Bme1580I BmgBI BmrI BpmI BsaHI BsaWI
BsaXI BseRI BsgI BsiHKAI Bsp1286I BspEI BspHI BspMI
BssHII BssSI BstAPI BstEII BtgI BtgZI EciI EcoICRI
Eco57MI EcoO109I FspI HaeII Hin4I KasI MslI MspA1I
NarI PfoI PpuMI PstI SacI SacII SanDI SexAI
SfcI SfoI SmaI Tth111I XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AloI ApaI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI BclI
BfrBI BglII BlpI BmtI BplI Bpu10I BpuEI BsaI
BsaAI BsaBI BseYI BsiEI BsiWI BsmI BsmBI BsrBI
BsrDI BsrFI BsrGI BstBI BstXI BstYI BstZ17I Bsu36I
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EcoNI EcoRI EcoRV FalI FseI FspAI HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI NaeI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PflMI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SalI
SapI SbfI ScaI SfiI SgfI SgrAI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI SwaI TaqII
TatI TstI XbaI XcmI XhoI XmnI ZraI