Restriction map for
pF1KB8878
(Linear) (Six-base) MAPSORT of: KIBB8878.seq Check: 1954 from: 1 to: 507
KIBB8878.orf
With 182 enzymes: SgfI *
March 2, 2010 14:29 ..
Acc65I G'GTAC_C
Cuts at: 0 106 507
Size: 106 401
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 74 425 507
Size: 74 351 82
Fragments arranged by size:
351 82 74
AvaI C'yCGr_G
Cuts at: 0 338 507
Size: 338 169
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 98 131 507
Size: 98 33 376
Fragments arranged by size:
376 98 33
BanI G'GyrC_C
Cuts at: 0 106 507
Size: 106 401
BanII G_rGCy'C
Cuts at: 0 238 265 474 507
Size: 238 27 209 33
Fragments arranged by size:
238 209 33 27
BbsI GAAGACnn'nnnn_
Cuts at: 0 80 296 507
Size: 80 216 211
Fragments arranged by size:
216 211 80
BmtI G_CTAG'C
Cuts at: 0 403 507
Size: 403 104
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 230 507
Size: 230 277
Bpu10I CC'TnA_GC
Cuts at: 0 272 507
Size: 272 235
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 406 507
Size: 406 101
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 208 211 298 507
Size: 208 3 87 209
Fragments arranged by size:
209 208 87 3
BseYI C'CCAG_C
Cuts at: 0 44 333 507
Size: 44 289 174
Fragments arranged by size:
289 174 44
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 481 507
Size: 481 26
BsiHKAI G_wGCw'C
Cuts at: 0 265 474 507
Size: 265 209 33
Fragments arranged by size:
265 209 33
Bsp1286I G_dGCh'C
Cuts at: 0 238 265 474 507
Size: 238 27 209 33
Fragments arranged by size:
238 209 33 27
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 80 507
Size: 80 427
BsrDI GCAATG_nn'
Cuts at: 0 13 507
Size: 13 494
EarI CTCTTCn'nnn_
Cuts at: 0 128 275 401 507
Size: 128 147 126 106
Fragments arranged by size:
147 128 126 106
EcoICRI GAG'CTC
Cuts at: 0 263 472 507
Size: 263 209 35
Fragments arranged by size:
263 209 35
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 74 230 425 507
Size: 74 156 195 82
Fragments arranged by size:
195 156 82 74
EcoNI CCTnn'n_nnAGG
Cuts at: 0 76 271 507
Size: 76 195 236
Fragments arranged by size:
236 195 76
EcoO109I rG'GnC_Cy
Cuts at: 0 69 81 507
Size: 69 12 426
Fragments arranged by size:
426 69 12
HaeII r_GCGC'y
Cuts at: 0 441 507
Size: 441 66
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 70 102 127 159 507
Size: 70 32 25 32 348
Fragments arranged by size:
348 70 32 32 25
KpnI G_GTAC'C
Cuts at: 0 110 507
Size: 110 397
MspA1I CmG'CkG
Cuts at: 0 7 44 507
Size: 7 37 463
Fragments arranged by size:
463 37 7
NheI G'CTAG_C
Cuts at: 0 399 507
Size: 399 108
PfoI T'CCnGG_A
Cuts at: 0 123 507
Size: 123 384
PpuMI rG'GwC_Cy
Cuts at: 0 69 81 507
Size: 69 12 426
Fragments arranged by size:
426 69 12
PvuII CAG'CTG
Cuts at: 0 7 44 507
Size: 7 37 463
Fragments arranged by size:
463 37 7
SacI G_AGCT'C
Cuts at: 0 265 474 507
Size: 265 209 33
Fragments arranged by size:
265 209 33
SmaI CCC'GGG
Cuts at: 0 340 507
Size: 340 167
SmlI C'TyrA_G
Cuts at: 0 421 507
Size: 421 86
SrfI GCCC'GGGC
Cuts at: 0 340 507
Size: 340 167
StyI C'CwwG_G
Cuts at: 0 294 507
Size: 294 213
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 497 507
Size: 497 10
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 306 338 507
Size: 306 32 169
Fragments arranged by size:
306 169 32
Tth111I GACn'n_nGTC
Cuts at: 0 36 507
Size: 36 471
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 48 507
Size: 48 459
XmaI C'CCGG_G
Cuts at: 0 338 507
Size: 338 169
Enzymes that do cut:
Acc65I AcuI AvaI BaeI BanI BanII BbsI BmtI
BpmI Bpu10I BpuEI BseRI BseYI BsgI BsiHKAI Bsp1286I
BspMI BsrDI EarI EcoICRI Eco57MI EcoNI EcoO109I HaeII
Hin4I KpnI MspA1I NheI PfoI PpuMI PvuII SacI
SmaI SmlI SrfI StyI TaqII TstI Tth111I XcmI
XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvrII BamHI BbeI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EciI EcoRI EcoRV FalI
FseI FspI FspAI HincII HindIII HpaI KasI MfeI
MluI MmeI MscI MslI NaeI NarI NcoI NdeI
NgoMIV NotI NruI NsiI NspI PacI PasI PciI
PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI RsrII SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SnaBI SpeI SphI SspI StuI SwaI TatI XbaI
XhoI XmnI ZraI