(Linear) (Six-base) MAPSORT of: KIBB8877.seq  Check: 633  from: 1  to: 504



KIBB8877.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:29  ..

AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     344     504 
   Size:        344     160
 
AvaI C'yCGr_G

Cuts at:      0     148     504 
   Size:        148     356
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     289     504 
   Size:        289     215
 
BsaHI Gr'CG_yC

Cuts at:      0      28     504 
   Size:         28     476
 
BseYI C'CCAG_C

Cuts at:      0     481     504 
   Size:        481      23
 
BspHI T'CATG_A

Cuts at:      0     222     504 
   Size:        222     282
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     117     229     320     504 
   Size:        117     112      91     184

  Fragments arranged by size: 

                 184     117     112      91
 
BsrDI GCAATG_nn'

Cuts at:      0     390     504 
   Size:        390     114
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     387     504 
   Size:        387     117
 
EaeI y'GGCC_r

Cuts at:      0       2     230     377     428     504 
   Size:          2     228     147      51      76

  Fragments arranged by size: 

                 228     147      76      51       2
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     289     504 
   Size:        289     215
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     466     504 
   Size:        466      38
 
EcoO109I rG'GnC_Cy

Cuts at:      0      48     504 
   Size:         48     456
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      13      45     504 
   Size:         13      32     459

  Fragments arranged by size: 

                 459      32      13
 
HincII GTy'rAC

Cuts at:      0     288     504 
   Size:        288     216
 
HpaI GTT'AAC

Cuts at:      0     288     504 
   Size:        288     216
 
MscI TGG'CCA

Cuts at:      0     430     504 
   Size:        430      74
 
MspA1I CmG'CkG

Cuts at:      0      37     504 
   Size:         37     467
 
PfoI T'CCnGG_A

Cuts at:      0     245     504 
   Size:        245     259
 
PpuMI rG'GwC_Cy

Cuts at:      0      48     504 
   Size:         48     456
 
PstI C_TGCA'G

Cuts at:      0     128     504 
   Size:        128     376
 
PvuII CAG'CTG

Cuts at:      0      37     504 
   Size:         37     467
 
SfcI C'TryA_G

Cuts at:      0     124     504 
   Size:        124     380
 
SpeI A'CTAG_T

Cuts at:      0      64     504 
   Size:         64     440
 
StyI C'CwwG_G

Cuts at:      0      10     504 
   Size:         10     494
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     215     504 
   Size:        215     289
 

 Enzymes that do cut:

     AlfI     AvaI     BpmI    BsaHI    BseYI    BspHI    BspMI    BsrDI
    BstXI     EaeI  Eco57MI    EcoNI EcoO109I    Hin4I   HincII     HpaI
     MscI   MspA1I     PfoI    PpuMI     PstI    PvuII     SfcI     SpeI
     StyI    TaqII

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaWI    BsaXI    BseRI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI
    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EarI     EciI  EcoICRI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII  HindIII     KasI
     KpnI     MfeI     MluI     MmeI     MslI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SphI     SrfI     SspI     StuI
     SwaI     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI