Restriction map for
pF1KB8875
(Linear) (Six-base) MAPSORT of: KIBB8875.seq Check: 2808 from: 1 to: 498
KIBB8875.orf
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
Acc65I G'GTAC_C
Cuts at: 0 220 498
Size: 220 278
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 289 498
Size: 202 87 209
Fragments arranged by size:
209 202 87
AleI CACnn'nnGTG
Cuts at: 0 361 498
Size: 361 137
AvaI C'yCGr_G
Cuts at: 0 50 498
Size: 50 448
BanI G'GyrC_C
Cuts at: 0 190 220 313 498
Size: 190 30 93 185
Fragments arranged by size:
190 185 93 30
BanII G_rGCy'C
Cuts at: 0 453 498
Size: 453 45
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 147 181 498
Size: 147 34 317
Fragments arranged by size:
317 147 34
Bme1580I G_kGCm'C
Cuts at: 0 324 498
Size: 324 174
BmgBI CAC'GTC
Cuts at: 0 60 498
Size: 60 438
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 467 498
Size: 467 31
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 193 498
Size: 28 165 305
Fragments arranged by size:
305 165 28
Bpu10I CC'TnA_GC
Cuts at: 0 452 498
Size: 452 46
BsaI GGTCTCn'nnnn_
Cuts at: 0 421 498
Size: 421 77
BsaBI GATnn'nnATC
Cuts at: 0 395 498
Size: 395 103
BsaHI Gr'CG_yC
Cuts at: 0 107 407 498
Size: 107 300 91
Fragments arranged by size:
300 107 91
BsaWI w'CCGG_w
Cuts at: 0 217 498
Size: 217 281
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 25 498
Size: 25 473
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 82 498
Size: 67 15 416
Fragments arranged by size:
416 67 15
BsiEI CG_ry'CG
Cuts at: 0 30 498
Size: 30 468
BsiHKAI G_wGCw'C
Cuts at: 0 287 498
Size: 287 211
Bsp1286I G_dGCh'C
Cuts at: 0 287 324 453 498
Size: 287 37 129 45
Fragments arranged by size:
287 129 45 37
BspHI T'CATG_A
Cuts at: 0 143 498
Size: 143 355
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 129 498
Size: 129 369
BsrFI r'CCGG_y
Cuts at: 0 22 29 498
Size: 22 7 469
Fragments arranged by size:
469 22 7
BssSI C'ACGA_G
Cuts at: 0 460 498
Size: 460 38
BstAPI GCAn_nnn'nTGC
Cuts at: 0 69 498
Size: 69 429
BstXI CCAn_nnnn'nTGG
Cuts at: 0 171 498
Size: 171 327
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 150 382 498
Size: 150 232 116
Fragments arranged by size:
232 150 116
BtsI GCAGTG_nn'
Cuts at: 0 356 436 498
Size: 356 80 62
Fragments arranged by size:
356 80 62
DraIII CAC_nnn'GTG
Cuts at: 0 486 498
Size: 486 12
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 193 202 289 498
Size: 28 165 9 87 209
Fragments arranged by size:
209 165 87 28 9
EcoO109I rG'GnC_Cy
Cuts at: 0 273 456 498
Size: 273 183 42
Fragments arranged by size:
273 183 42
HaeII r_GCGC'y
Cuts at: 0 136 498
Size: 136 362
HincII GTy'rAC
Cuts at: 0 300 498
Size: 300 198
KpnI G_GTAC'C
Cuts at: 0 224 498
Size: 224 274
MslI CAynn'nnrTG
Cuts at: 0 361 498
Size: 361 137
MspA1I CmG'CkG
Cuts at: 0 382 495 498
Size: 382 113 3
Fragments arranged by size:
382 113 3
NaeI GCC'GGC
Cuts at: 0 24 498
Size: 24 474
NgoMIV G'CCGG_C
Cuts at: 0 22 498
Size: 22 476
PflMI CCAn_nnn'nTGG
Cuts at: 0 486 498
Size: 486 12
PfoI T'CCnGG_A
Cuts at: 0 206 257 498
Size: 206 51 241
Fragments arranged by size:
241 206 51
PpuMI rG'GwC_Cy
Cuts at: 0 273 456 498
Size: 273 183 42
Fragments arranged by size:
273 183 42
PstI C_TGCA'G
Cuts at: 0 140 443 476 498
Size: 140 303 33 22
Fragments arranged by size:
303 140 33 22
PvuII CAG'CTG
Cuts at: 0 382 498
Size: 382 116
SbfI CC_TGCA'GG
Cuts at: 0 476 498
Size: 476 22
SfcI C'TryA_G
Cuts at: 0 136 439 472 498
Size: 136 303 33 26
Fragments arranged by size:
303 136 33 26
SgrAI Cr'CCGG_yG
Cuts at: 0 22 498
Size: 22 476
SmaI CCC'GGG
Cuts at: 0 52 498
Size: 52 446
StyI C'CwwG_G
Cuts at: 0 425 498
Size: 425 73
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 314 346 498
Size: 314 32 152
Fragments arranged by size:
314 152 32
XmaI C'CCGG_G
Cuts at: 0 50 498
Size: 50 448
Enzymes that do cut:
Acc65I AcuI AleI AvaI BanI BanII BcgI Bme1580I
BmgBI BplI BpmI Bpu10I BsaI BsaBI BsaHI BsaWI
BseRI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrFI
BssSI BstAPI BstXI BtgZI BtsI DraIII Eco57MI EcoO109I
HaeII HincII KpnI MslI MspA1I NaeI NgoMIV PflMI
PfoI PpuMI PstI PvuII SbfI SfcI SgrAI SmaI
StyI TstI XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbeI BbsI BbvCI
BciVI BclI BfrBI BglI BglII BlpI BmrI BmtI
BpuEI BsaAI BsaXI BseYI BsiWI BsmI BsmBI BspEI
BsrBI BsrDI BsrGI BssHII BstBI BstEII BstYI BstZ17I
Bsu36I BtgI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI Hin4I HindIII HpaI KasI MfeI MluI
MmeI MscI NarI NcoI NdeI NheI NotI NruI
NsiI NspI PacI PasI PciI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SapI ScaI SexAI SfiI SfoI
SgfI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI TaqII TatI Tth111I XbaI XcmI XhoI XmnI
ZraI