Restriction map for
pF1KB8873
(Linear) (Six-base) MAPSORT of: KIBB8873.seq Check: 9800 from: 1 to: 486
KIBB8873.orf
With 182 enzymes: SgfI *
March 3, 2008 11:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 128 486
Size: 128 358
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 175 486
Size: 175 311
AlwNI CAG_nnn'CTG
Cuts at: 0 120 486
Size: 120 366
ApaI G_GGCC'C
Cuts at: 0 394 416 486
Size: 394 22 70
Fragments arranged by size:
394 70 22
AvaI C'yCGr_G
Cuts at: 0 386 486
Size: 386 100
BanI G'GyrC_C
Cuts at: 0 61 378 486
Size: 61 317 108
Fragments arranged by size:
317 108 61
BanII G_rGCy'C
Cuts at: 0 311 394 416 486
Size: 311 83 22 70
Fragments arranged by size:
311 83 70 22
BbeI G_GCGC'C
Cuts at: 0 382 486
Size: 382 104
Bme1580I G_kGCm'C
Cuts at: 0 394 416 486
Size: 394 22 70
Fragments arranged by size:
394 70 22
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 325 486
Size: 256 69 161
Fragments arranged by size:
256 161 69
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 486
Size: 160 326
BsaHI Gr'CG_yC
Cuts at: 0 379 486
Size: 379 107
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 94 154 244 486
Size: 94 60 90 242
Fragments arranged by size:
242 94 90 60
BsiEI CG_ry'CG
Cuts at: 0 484 486
Size: 484 2
BsiHKAI G_wGCw'C
Cuts at: 0 311 486
Size: 311 175
Bsp1286I G_dGCh'C
Cuts at: 0 311 394 416 486
Size: 311 83 22 70
Fragments arranged by size:
311 83 70 22
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 128 486
Size: 128 358
BsrFI r'CCGG_y
Cuts at: 0 164 403 486
Size: 164 239 83
Fragments arranged by size:
239 164 83
BstEII G'GTnAC_C
Cuts at: 0 87 486
Size: 87 399
BtgI C'CryG_G
Cuts at: 0 183 374 486
Size: 183 191 112
Fragments arranged by size:
191 183 112
EaeI y'GGCC_r
Cuts at: 0 434 486
Size: 434 52
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 265 486
Size: 265 221
EcoICRI GAG'CTC
Cuts at: 0 309 486
Size: 309 177
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 175 256 325 486
Size: 175 81 69 161
Fragments arranged by size:
175 161 81 69
EcoNI CCTnn'n_nnAGG
Cuts at: 0 133 486
Size: 133 353
EcoO109I rG'GnC_Cy
Cuts at: 0 126 390 391 412 413 486
Size: 126 264 1 21 1 73
Fragments arranged by size:
264 126 73 21 1 1
HaeII r_GCGC'y
Cuts at: 0 165 340 382 486
Size: 165 175 42 104
Fragments arranged by size:
175 165 104 42
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 466 486
Size: 466 20
KasI G'GCGC_C
Cuts at: 0 378 486
Size: 378 108
MslI CAynn'nnrTG
Cuts at: 0 97 486
Size: 97 389
MspA1I CmG'CkG
Cuts at: 0 153 185 200 486
Size: 153 32 15 286
Fragments arranged by size:
286 153 32 15
NaeI GCC'GGC
Cuts at: 0 166 405 486
Size: 166 239 81
Fragments arranged by size:
239 166 81
NarI GG'CG_CC
Cuts at: 0 379 486
Size: 379 107
NgoMIV G'CCGG_C
Cuts at: 0 164 403 486
Size: 164 239 83
Fragments arranged by size:
239 164 83
PspOMI G'GGCC_C
Cuts at: 0 390 412 486
Size: 390 22 74
Fragments arranged by size:
390 74 22
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 172 204 486
Size: 172 32 282
Fragments arranged by size:
282 172 32
PstI C_TGCA'G
Cuts at: 0 260 344 486
Size: 260 84 142
Fragments arranged by size:
260 142 84
PvuI CG_AT'CG
Cuts at: 0 484 486
Size: 484 2
PvuII CAG'CTG
Cuts at: 0 153 200 486
Size: 153 47 286
Fragments arranged by size:
286 153 47
SacI G_AGCT'C
Cuts at: 0 311 486
Size: 311 175
SacII CC_GC'GG
Cuts at: 0 186 486
Size: 186 300
SfcI C'TryA_G
Cuts at: 0 256 340 486
Size: 256 84 146
Fragments arranged by size:
256 146 84
SfoI GGC'GCC
Cuts at: 0 380 486
Size: 380 106
SgrAI Cr'CCGG_yG
Cuts at: 0 164 486
Size: 164 322
SmaI CCC'GGG
Cuts at: 0 388 486
Size: 388 98
SmlI C'TyrA_G
Cuts at: 0 175 486
Size: 175 311
StuI AGG'CCT
Cuts at: 0 28 486
Size: 28 458
StyI C'CwwG_G
Cuts at: 0 134 486
Size: 134 352
Tth111I GACn'n_nGTC
Cuts at: 0 93 486
Size: 93 393
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 300 486
Size: 300 186
XmaI C'CCGG_G
Cuts at: 0 386 486
Size: 386 100
Enzymes that do cut:
AarI AcuI AlwNI ApaI AvaI BanI BanII BbeI
Bme1580I BpmI BpuEI BsaHI BseRI BsiEI BsiHKAI Bsp1286I
BspMI BsrFI BstEII BtgI EaeI EciI EcoICRI Eco57MI
EcoNI EcoO109I HaeII Hin4I KasI MslI MspA1I NaeI
NarI NgoMIV PspOMI PsrI PstI PvuI PvuII SacI
SacII SfcI SfoI SgrAI SmaI SmlI StuI StyI
Tth111I XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI BmgBI BmrI BmtI
BplI Bpu10I BsaI BsaAI BsaBI BsaWI BsaXI BseYI
BsgI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI
BsrGI BssHII BssSI BstAPI BstBI BstXI BstYI BstZ17I
Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EagI EarI EcoRI EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
NcoI NdeI NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspXI RsrII SalI SanDI SapI SbfI
ScaI SexAI SfiI SgfI SnaBI SpeI SphI SrfI
SspI SwaI TaqII TatI TstI XbaI XhoI XmnI
ZraI