Restriction map for
pF1KB8868
(Linear) (Six-base) MAPSORT of: KIBB8868.seq Check: 3036 from: 1 to: 378
KIBB8868.orf
With 182 enzymes: SgfI *
March 3, 2008 11:09 ..
AgeI A'CCGG_T
Cuts at: 0 200 378
Size: 200 178
AhdI GACnn_n'nnGTC
Cuts at: 0 237 378
Size: 237 141
AlwNI CAG_nnn'CTG
Cuts at: 0 18 378
Size: 18 360
AvaI C'yCGr_G
Cuts at: 0 73 169 378
Size: 73 96 209
Fragments arranged by size:
209 96 73
BanI G'GyrC_C
Cuts at: 0 78 181 378
Size: 78 103 197
Fragments arranged by size:
197 103 78
BanII G_rGCy'C
Cuts at: 0 258 378
Size: 258 120
BbeI G_GCGC'C
Cuts at: 0 82 185 378
Size: 82 103 193
Fragments arranged by size:
193 103 82
BfrBI ATG'CAT
Cuts at: 0 3 378
Size: 3 375
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 175 322 378
Size: 175 147 56
Fragments arranged by size:
175 147 56
BsaHI Gr'CG_yC
Cuts at: 0 79 182 378
Size: 79 103 196
Fragments arranged by size:
196 103 79
BsaWI w'CCGG_w
Cuts at: 0 200 378
Size: 200 178
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 127 378
Size: 127 251
BseYI C'CCAG_C
Cuts at: 0 11 378
Size: 11 367
BsiEI CG_ry'CG
Cuts at: 0 201 205 378
Size: 201 4 173
Fragments arranged by size:
201 173 4
BsiHKAI G_wGCw'C
Cuts at: 0 275 378
Size: 275 103
Bsp1286I G_dGCh'C
Cuts at: 0 258 275 378
Size: 258 17 103
Fragments arranged by size:
258 103 17
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 230 378
Size: 230 148
BsrBI CCG'CTC
Cuts at: 0 365 378
Size: 365 13
BsrFI r'CCGG_y
Cuts at: 0 105 200 378
Size: 105 95 178
Fragments arranged by size:
178 105 95
BssHII G'CGCG_C
Cuts at: 0 283 378
Size: 283 95
EaeI y'GGCC_r
Cuts at: 0 119 378
Size: 119 259
EcoO109I rG'GnC_Cy
Cuts at: 0 90 219 378
Size: 90 129 159
Fragments arranged by size:
159 129 90
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 330 345 378
Size: 330 15 33
Fragments arranged by size:
330 33 15
HaeII r_GCGC'y
Cuts at: 0 82 185 378
Size: 82 103 193
Fragments arranged by size:
193 103 82
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 223 255 378
Size: 223 32 123
Fragments arranged by size:
223 123 32
KasI G'GCGC_C
Cuts at: 0 78 181 378
Size: 78 103 197
Fragments arranged by size:
197 103 78
MspA1I CmG'CkG
Cuts at: 0 15 378
Size: 15 363
NaeI GCC'GGC
Cuts at: 0 107 378
Size: 107 271
NarI GG'CG_CC
Cuts at: 0 79 182 378
Size: 79 103 196
Fragments arranged by size:
196 103 79
NgoMIV G'CCGG_C
Cuts at: 0 105 378
Size: 105 273
NruI TCG'CGA
Cuts at: 0 207 378
Size: 207 171
NsiI A_TGCA'T
Cuts at: 0 5 378
Size: 5 373
PflMI CCAn_nnn'nTGG
Cuts at: 0 18 378
Size: 18 360
PfoI T'CCnGG_A
Cuts at: 0 215 378
Size: 215 163
PpuMI rG'GwC_Cy
Cuts at: 0 90 219 378
Size: 90 129 159
Fragments arranged by size:
159 129 90
PvuII CAG'CTG
Cuts at: 0 15 378
Size: 15 363
SfoI GGC'GCC
Cuts at: 0 80 183 378
Size: 80 103 195
Fragments arranged by size:
195 103 80
SmaI CCC'GGG
Cuts at: 0 75 171 378
Size: 75 96 207
Fragments arranged by size:
207 96 75
SmlI C'TyrA_G
Cuts at: 0 190 337 378
Size: 190 147 41
Fragments arranged by size:
190 147 41
StyI C'CwwG_G
Cuts at: 0 320 378
Size: 320 58
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 362 378
Size: 362 16
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 366 378
Size: 366 12
XmaI C'CCGG_G
Cuts at: 0 73 169 378
Size: 73 96 209
Fragments arranged by size:
209 96 73
Enzymes that do cut:
AgeI AhdI AlwNI AvaI BanI BanII BbeI BfrBI
BpuEI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspMI BsrBI BsrFI BssHII EaeI EcoO109I FalI HaeII
Hin4I KasI MspA1I NaeI NarI NgoMIV NruI NsiI
PflMI PfoI PpuMI PvuII SfoI SmaI SmlI StyI
TaqII XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AleI AlfI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BsaI BsaAI BsaBI BsaXI
BsgI BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrGI
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV
FseI FspI FspAI HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI NcoI NdeI NheI NotI
NspI PacI PasI PciI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
StuI SwaI TatI TstI Tth111I XbaI XhoI XmnI
ZraI