(Linear) (Six-base) MAPSORT of: KIBB8867.seq  Check: 8642  from: 1  to: 366



KIBB8867.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:50  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      54     183     366 
   Size:         54     129     183

  Fragments arranged by size: 

                 183     129      54
 
AhdI GACnn_n'nnGTC

Cuts at:      0     228     366 
   Size:        228     138
 
AlwNI CAG_nnn'CTG

Cuts at:      0     275     366 
   Size:        275      91
 
BanII G_rGCy'C

Cuts at:      0      88     366 
   Size:         88     278
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     227     366 
   Size:        227     139
 
BbvCI CC'TCA_GC

Cuts at:      0     268     366 
   Size:        268      98
 
BglI GCCn_nnn'nGGC

Cuts at:      0     321     366 
   Size:        321      45
 
Bpu10I CC'TnA_GC

Cuts at:      0     268     366 
   Size:        268      98
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     162     366 
   Size:        162     204
 
BseYI C'CCAG_C

Cuts at:      0      17     209     366 
   Size:         17     192     157

  Fragments arranged by size: 

                 192     157      17
 
BsiHKAI G_wGCw'C

Cuts at:      0     161     366 
   Size:        161     205
 
Bsp1286I G_dGCh'C

Cuts at:      0      88     161     366 
   Size:         88      73     205

  Fragments arranged by size: 

                 205      88      73
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      54     183     366 
   Size:         54     129     183

  Fragments arranged by size: 

                 183     129      54
 
BstBI TT'CG_AA

Cuts at:      0     152     366 
   Size:        152     214
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     297     366 
   Size:        297      69
 
BtsI GCAGTG_nn'

Cuts at:      0      38     366 
   Size:         38     328
 
DraIII CAC_nnn'GTG

Cuts at:      0     165     366 
   Size:        165     201
 
EaeI y'GGCC_r

Cuts at:      0     287     366 
   Size:        287      79
 
EarI CTCTTCn'nnn_

Cuts at:      0     149     366 
   Size:        149     217
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     339     366 
   Size:        339      27
 
EcoO109I rG'GnC_Cy

Cuts at:      0      91     366 
   Size:         91     275
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     214     246     366 
   Size:        214      32     120

  Fragments arranged by size: 

                 214     120      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     366 
   Size:        366
 
MscI TGG'CCA

Cuts at:      0     289     366 
   Size:        289      77
 
MspA1I CmG'CkG

Cuts at:      0     244     366 
   Size:        244     122
 
PstI C_TGCA'G

Cuts at:      0      28      65     132     366 
   Size:         28      37      67     234

  Fragments arranged by size: 

                 234      67      37      28
 
PvuII CAG'CTG

Cuts at:      0     244     366 
   Size:        244     122
 
SapI GCTCTTCn'nnn_

Cuts at:      0     149     366 
   Size:        149     217
 
SfcI C'TryA_G

Cuts at:      0      24      61     128     366 
   Size:         24      37      67     238

  Fragments arranged by size: 

                 238      67      37      24
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     169     366 
   Size:        169     197
 

 Enzymes that do cut:

     AarI     AhdI    AlwNI    BanII     BbsI    BbvCI     BglI   Bpu10I
    BseRI    BseYI  BsiHKAI Bsp1286I    BspMI    BstBI    BstXI     BtsI
   DraIII     EaeI     EarI     EciI EcoO109I    Hin4I     MmeI     MscI
   MspA1I     PstI    PvuII     SapI     SfcI    TaqII

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI
     BcgI    BciVI     BclI    BfrBI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI     BsgI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI   BstEII    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI
    CspCI     DraI     DrdI     EagI  EcoICRI  Eco57MI    EcoNI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MslI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     StyI     SwaI     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI