(Linear) (Six-base) MAPSORT of: KIBB8864.seq  Check: 9432  from: 1  to: 330



KIBB8864.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:29  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     174     183     330 
   Size:        174       9     147

  Fragments arranged by size: 

                 174     147       9
 
AlwNI CAG_nnn'CTG

Cuts at:      0     296     330 
   Size:        296      34
 
ApaI G_GGCC'C

Cuts at:      0     214     330 
   Size:        214     116
 
BanII G_rGCy'C

Cuts at:      0     214     330 
   Size:        214     116
 
Bme1580I G_kGCm'C

Cuts at:      0     214     330 
   Size:        214     116
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     265     330 
   Size:        265      65
 
BseYI C'CCAG_C

Cuts at:      0      60     201     330 
   Size:         60     141     129

  Fragments arranged by size: 

                 141     129      60
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     239     330 
   Size:        239      91
 
Bsp1286I G_dGCh'C

Cuts at:      0     214     330 
   Size:        214     116
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     174     183     188     330 
   Size:        174       9       5     142

  Fragments arranged by size: 

                 174     142       9       5
 
DraIII CAC_nnn'GTG

Cuts at:      0     105     330 
   Size:        105     225
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     265     330 
   Size:        265      65
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     218     330 
   Size:        218     112
 
EcoO109I rG'GnC_Cy

Cuts at:      0      52     177     210     211     251     330 
   Size:         52     125      33       1      40      79

  Fragments arranged by size: 

                 125      79      52      40      33       1
 
HaeII r_GCGC'y

Cuts at:      0      37     330 
   Size:         37     293
 
MspA1I CmG'CkG

Cuts at:      0     312     330 
   Size:        312      18
 
PpuMI rG'GwC_Cy

Cuts at:      0      52     177     251     330 
   Size:         52     125      74      79

  Fragments arranged by size: 

                 125      79      74      52
 
PspOMI G'GGCC_C

Cuts at:      0     210     330 
   Size:        210     120
 
PstI C_TGCA'G

Cuts at:      0     185     233     260     330 
   Size:        185      48      27      70

  Fragments arranged by size: 

                 185      70      48      27
 
PvuII CAG'CTG

Cuts at:      0     312     330 
   Size:        312      18
 
SanDI GG'GwC_CC

Cuts at:      0     251     330 
   Size:        251      79
 
SbfI CC_TGCA'GG

Cuts at:      0     185     330 
   Size:        185     145
 
SexAI A'CCwGG_T

Cuts at:      0     101     330 
   Size:        101     229
 
SfcI C'TryA_G

Cuts at:      0     181     229     256     330 
   Size:        181      48      27      74

  Fragments arranged by size: 

                 181      74      48      27
 
StyI C'CwwG_G

Cuts at:      0     236     330 
   Size:        236      94
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     168     330 
   Size:        168     162
 

 Enzymes that do cut:

     AarI    AlwNI     ApaI    BanII Bme1580I     BpmI    BseYI     BsgI
 Bsp1286I    BspMI   DraIII  Eco57MI    EcoNI EcoO109I    HaeII   MspA1I
    PpuMI   PspOMI     PstI    PvuII    SanDI     SbfI    SexAI     SfcI
     StyI    TaqII

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII     SacI
    SacII     SalI     SapI     ScaI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI