(Linear) (Six-base) MAPSORT of: KIBB8863.seq  Check: 445  from: 1  to: 303



KIBB8863.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:09  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     171     303 
   Size:        171     132
 
ApoI r'AATT_y

Cuts at:      0     262     303 
   Size:        262      41
 
AvaI C'yCGr_G

Cuts at:      0      57     116     303 
   Size:         57      59     187

  Fragments arranged by size: 

                 187      59      57
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     222     303 
   Size:        222      81
 
Bme1580I G_kGCm'C

Cuts at:      0      10     303 
   Size:         10     293
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      34     303 
   Size:         34     269
 
BseYI C'CCAG_C

Cuts at:      0     127     303 
   Size:        127     176
 
Bsp1286I G_dGCh'C

Cuts at:      0      10     303 
   Size:         10     293
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     135     303 
   Size:        135     168
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     228     303 
   Size:        228      75
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      34     303 
   Size:         34     269
 
EcoO109I rG'GnC_Cy

Cuts at:      0      21     303 
   Size:         21     282
 
EcoRI G'AATT_C

Cuts at:      0     262     303 
   Size:        262      41
 
MslI CAynn'nnrTG

Cuts at:      0     247     303 
   Size:        247      56
 
ScaI AGT'ACT

Cuts at:      0      55     223     303 
   Size:         55     168      80

  Fragments arranged by size: 

                 168      80      55
 
SmaI CCC'GGG

Cuts at:      0     118     303 
   Size:        118     185
 
TatI w'GTAC_w

Cuts at:      0      53     221     303 
   Size:         53     168      82

  Fragments arranged by size: 

                 168      82      53
 
XmaI C'CCGG_G

Cuts at:      0     116     303 
   Size:        116     187
 

 Enzymes that do cut:

    AlwNI     ApoI     AvaI     BbsI Bme1580I     BpmI    BseYI Bsp1286I
    BstXI    BtgZI  Eco57MI EcoO109I    EcoRI     MslI     ScaI     SmaI
     TatI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI     BsgI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstYI  BstZ17I   Bsu36I     BtgI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     StyI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI
     XmnI     ZraI