(Linear) (Six-base) MAPSORT of: KIBB8860.seq  Check: 1437  from: 1  to: 291



KIBB8860.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:09  ..

AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      97     129     291 
   Size:         97      32     162

  Fragments arranged by size: 

                 162      97      32
 
ApoI r'AATT_y

Cuts at:      0      52     291 
   Size:         52     239
 
BseYI C'CCAG_C

Cuts at:      0      58     291 
   Size:         58     233
 
BsrDI GCAATG_nn'

Cuts at:      0     239     255     291 
   Size:        239      16      36

  Fragments arranged by size: 

                 239      36      16
 
BtgI C'CryG_G

Cuts at:      0      23     291 
   Size:         23     268
 
EcoO109I rG'GnC_Cy

Cuts at:      0      87     171     291 
   Size:         87      84     120

  Fragments arranged by size: 

                 120      87      84
 
NcoI C'CATG_G

Cuts at:      0      23     291 
   Size:         23     268
 
NspI r_CATG'y

Cuts at:      0     248     291 
   Size:        248      43
 
PpuMI rG'GwC_Cy

Cuts at:      0      87     171     291 
   Size:         87      84     120

  Fragments arranged by size: 

                 120      87      84
 
ScaI AGT'ACT

Cuts at:      0      98     291 
   Size:         98     193
 
SphI G_CATG'C

Cuts at:      0     248     291 
   Size:        248      43
 
StyI C'CwwG_G

Cuts at:      0      23     291 
   Size:         23     268
 
TatI w'GTAC_w

Cuts at:      0      96     264     291 
   Size:         96     168      27

  Fragments arranged by size: 

                 168      96      27
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     187     291 
   Size:        187     104
 

 Enzymes that do cut:

     AloI     ApoI    BseYI    BsrDI     BtgI EcoO109I     NcoI     NspI
    PpuMI     ScaI     SphI     StyI     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SrfI     SspI     StuI     SwaI    TaqII     TstI
  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI