(Linear) (Six-base) MAPSORT of: KIBB8857.seq  Check: 5069  from: 1  to: 276



KIBB8857.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:09  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     103     118     276 
   Size:        103      15     158

  Fragments arranged by size: 

                 158     103      15
 
ApoI r'AATT_y

Cuts at:      0     217     276 
   Size:        217      59
 
BanII G_rGCy'C

Cuts at:      0     107     276 
   Size:        107     169
 
BglI GCCn_nnn'nGGC

Cuts at:      0      25     276 
   Size:         25     251
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      31     276 
   Size:         31     245
 
BsiHKAI G_wGCw'C

Cuts at:      0     107     276 
   Size:        107     169
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     188     276 
   Size:        188      88
 
Bsp1286I G_dGCh'C

Cuts at:      0     107     276 
   Size:        107     169
 
BssSI C'ACGA_G

Cuts at:      0     256     276 
   Size:        256      20
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      55     276 
   Size:         55     221
 
BtgI C'CryG_G

Cuts at:      0      20     236     276 
   Size:         20     216      40

  Fragments arranged by size: 

                 216      40      20
 
EcoICRI GAG'CTC

Cuts at:      0     105     276 
   Size:        105     171
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      31     103     118     276 
   Size:         31      72      15     158

  Fragments arranged by size: 

                 158      72      31      15
 
EcoRI G'AATT_C

Cuts at:      0     217     276 
   Size:        217      59
 
NcoI C'CATG_G

Cuts at:      0      20     236     276 
   Size:         20     216      40

  Fragments arranged by size: 

                 216      40      20
 
PfoI T'CCnGG_A

Cuts at:      0     212     276 
   Size:        212      64
 
SacI G_AGCT'C

Cuts at:      0     107     276 
   Size:        107     169
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0      25     276 
   Size:         25     251
 
SspI AAT'ATT

Cuts at:      0      52     276 
   Size:         52     224
 
StyI C'CwwG_G

Cuts at:      0      20     236     276 
   Size:         20     216      40

  Fragments arranged by size: 

                 216      40      20
 
Tth111I GACn'n_nGTC

Cuts at:      0     166     276 
   Size:        166     110
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      52     276 
   Size:         52     224
 

 Enzymes that do cut:

     AcuI     ApoI    BanII     BglI     BpmI  BsiHKAI    BsmBI Bsp1286I
    BssSI    BstXI     BtgI  EcoICRI  Eco57MI    EcoRI     NcoI     PfoI
     SacI     SfiI     SspI     StyI  Tth111I     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI    BsiWI     BsmI    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII   BstAPI    BstBI   BstEII    BstYI
  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EarI     EciI    EcoNI EcoO109I    EcoRV
     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     StuI     SwaI    TaqII     TatI
     TstI     XbaI     XhoI     XmaI     XmnI     ZraI