(Linear) (Six-base) MAPSORT of: KIBB8847.seq  Check: 8894  from: 1  to: 201



KIBB8847.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:50  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     124     201 
   Size:        124      77
 
AvaI C'yCGr_G

Cuts at:      0      94     201 
   Size:         94     107
 
BamHI G'GATC_C

Cuts at:      0     122     201 
   Size:        122      79
 
BmgBI CAC'GTC

Cuts at:      0      15     201 
   Size:         15     186
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      31     201 
   Size:         31     170
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      19     201 
   Size:         19     182
 
BstYI r'GATC_y

Cuts at:      0     122     201 
   Size:        122      79
 
BtgI C'CryG_G

Cuts at:      0     169     201 
   Size:        169      32
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     124     201 
   Size:        124      77
 
EcoO109I rG'GnC_Cy

Cuts at:      0     132     201 
   Size:        132      69
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     153     201 
   Size:        153      48
 
MspA1I CmG'CkG

Cuts at:      0      57      69     201 
   Size:         57      12     132

  Fragments arranged by size: 

                 132      57      12
 
NcoI C'CATG_G

Cuts at:      0     169     201 
   Size:        169      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     132     201 
   Size:        132      69
 
PvuII CAG'CTG

Cuts at:      0      57     201 
   Size:         57     144
 
SmlI C'TyrA_G

Cuts at:      0      46     201 
   Size:         46     155
 
StyI C'CwwG_G

Cuts at:      0     128     169     201 
   Size:        128      41      32

  Fragments arranged by size: 

                 128      41      32
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     162     201 
   Size:        162      39
 

 Enzymes that do cut:

     AcuI     AvaI    BamHI    BmgBI    BpuEI    BseRI    BstYI     BtgI
  Eco57MI EcoO109I     FalI   MspA1I     NcoI    PpuMI    PvuII     SmlI
     StyI    TaqII

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I     BmrI     BmtI     BplI     BpmI   Bpu10I
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI     BsgI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI    EcoNI    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII
    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     MslI     NaeI     NarI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI