Restriction map for
pF1KB8846
(Linear) (Six-base) MAPSORT of: KIBB8846.seq Check: 1763 from: 1 to: 1605
KIBB8846.orf
With 182 enzymes: SgfI *
March 3, 2008 11:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1476 1605
Size: 1476 129
AccI GT'mk_AC
Cuts at: 0 667 806 1531 1605
Size: 667 139 725 74
Fragments arranged by size:
725 667 139 74
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 592 960 1605
Size: 592 368 645
Fragments arranged by size:
645 592 368
AflIII A'CryG_T
Cuts at: 0 621 1168 1605
Size: 621 547 437
Fragments arranged by size:
621 547 437
AhdI GACnn_n'nnGTC
Cuts at: 0 534 1605
Size: 534 1071
AlwNI CAG_nnn'CTG
Cuts at: 0 1506 1557 1605
Size: 1506 51 48
Fragments arranged by size:
1506 51 48
ApoI r'AATT_y
Cuts at: 0 1032 1605
Size: 1032 573
AvaI C'yCGr_G
Cuts at: 0 489 787 897 1605
Size: 489 298 110 708
Fragments arranged by size:
708 489 298 110
BanI G'GyrC_C
Cuts at: 0 871 1605
Size: 871 734
BanII G_rGCy'C
Cuts at: 0 292 354 580 898 1605
Size: 292 62 226 318 707
Fragments arranged by size:
707 318 292 226 62
BbvCI CC'TCA_GC
Cuts at: 0 321 1605
Size: 321 1284
BciVI GTATCCnnnnn_n'
Cuts at: 0 704 1605
Size: 704 901
BglI GCCn_nnn'nGGC
Cuts at: 0 417 1006 1605
Size: 417 589 599
Fragments arranged by size:
599 589 417
BglII A'GATC_T
Cuts at: 0 833 1605
Size: 833 772
Bme1580I G_kGCm'C
Cuts at: 0 277 1605
Size: 277 1328
BmgBI CAC'GTC
Cuts at: 0 345 374 1605
Size: 345 29 1231
Fragments arranged by size:
1231 345 29
BmrI ACTGGGnnnn_n'
Cuts at: 0 1164 1462 1605
Size: 1164 298 143
Fragments arranged by size:
1164 298 143
BmtI G_CTAG'C
Cuts at: 0 97 1195 1605
Size: 97 1098 410
Fragments arranged by size:
1098 410 97
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 517 1605
Size: 517 1088
Bpu10I CC'TnA_GC
Cuts at: 0 321 1605
Size: 321 1284
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 749 1278 1605
Size: 749 529 327
Fragments arranged by size:
749 529 327
BsaI GGTCTCn'nnnn_
Cuts at: 0 430 535 590 1427 1605
Size: 430 105 55 837 178
Fragments arranged by size:
837 430 178 105 55
BsaAI yAC'GTr
Cuts at: 0 548 1005 1605
Size: 548 457 600
Fragments arranged by size:
600 548 457
BsaHI Gr'CG_yC
Cuts at: 0 509 1605
Size: 509 1096
BsaWI w'CCGG_w
Cuts at: 0 512 1605
Size: 512 1093
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 295 325 705 735 1605
Size: 295 30 380 30 870
Fragments arranged by size:
870 380 295 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 303 306 309 448 752 1135 1605
Size: 303 3 3 139 304 383 470
Fragments arranged by size:
470 383 304 303 139 3 3
BsiEI CG_ry'CG
Cuts at: 0 1242 1605
Size: 1242 363
BsiHKAI G_wGCw'C
Cuts at: 0 292 354 580 1304 1605
Size: 292 62 226 724 301
Fragments arranged by size:
724 301 292 226 62
BsmI GAATG_Cn'
Cuts at: 0 1377 1605
Size: 1377 228
Bsp1286I G_dGCh'C
Cuts at: 0 277 292 354 580 898 1304 1605
Size: 277 15 62 226 318 406 301
Fragments arranged by size:
406 318 301 277 226 62 15
BspEI T'CCGG_A
Cuts at: 0 512 1605
Size: 512 1093
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 448 608 1476 1605
Size: 448 160 868 129
Fragments arranged by size:
868 448 160 129
BsrBI CCG'CTC
Cuts at: 0 904 1605
Size: 904 701
BsrDI GCAATG_nn'
Cuts at: 0 934 1605
Size: 934 671
BssHII G'CGCG_C
Cuts at: 0 777 1605
Size: 777 828
BstYI r'GATC_y
Cuts at: 0 833 1435 1605
Size: 833 602 170
Fragments arranged by size:
833 602 170
BstZ17I GTA'TAC
Cuts at: 0 668 1532 1605
Size: 668 864 73
Fragments arranged by size:
864 668 73
Bsu36I CC'TnA_GG
Cuts at: 0 650 1605
Size: 650 955
BtgI C'CryG_G
Cuts at: 0 406 740 875 1097 1272 1605
Size: 406 334 135 222 175 333
Fragments arranged by size:
406 334 333 222 175 135
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 74 1605
Size: 74 1531
DraI TTT'AAA
Cuts at: 0 339 1605
Size: 339 1266
DrdI GACnn_nn'nnGTC
Cuts at: 0 820 1605
Size: 820 785
EaeI y'GGCC_r
Cuts at: 0 152 1605
Size: 152 1453
EarI CTCTTCn'nnn_
Cuts at: 0 486 568 738 1605
Size: 486 82 170 867
Fragments arranged by size:
867 486 170 82
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 203 1605
Size: 203 1402
EcoICRI GAG'CTC
Cuts at: 0 290 352 578 1605
Size: 290 62 226 1027
Fragments arranged by size:
1027 290 226 62
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 517 592 960 1605
Size: 517 75 368 645
Fragments arranged by size:
645 517 368 75
EcoNI CCTnn'n_nnAGG
Cuts at: 0 701 1117 1605
Size: 701 416 488
Fragments arranged by size:
701 488 416
EcoO109I rG'GnC_Cy
Cuts at: 0 813 1605
Size: 813 792
EcoRV GAT'ATC
Cuts at: 0 165 1605
Size: 165 1440
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 295 327 520 552 705 737 891 923
Size: 295 32 193 32 153 32 154 32
Cuts at: 923 1030 1062 1605
Size: 107 32 543
Fragments arranged by size:
543 295 193 154 153 107 32 32
32 32 32
HindIII A'AGCT_T
Cuts at: 0 1254 1605
Size: 1254 351
MscI TGG'CCA
Cuts at: 0 154 1605
Size: 154 1451
MslI CAynn'nnrTG
Cuts at: 0 448 614 914 1102 1307 1486 1605
Size: 448 166 300 188 205 179 119
Fragments arranged by size:
448 300 205 188 179 166 119
MspA1I CmG'CkG
Cuts at: 0 158 1605
Size: 158 1447
NcoI C'CATG_G
Cuts at: 0 740 1097 1272 1605
Size: 740 357 175 333
Fragments arranged by size:
740 357 333 175
NheI G'CTAG_C
Cuts at: 0 93 1191 1605
Size: 93 1098 414
Fragments arranged by size:
1098 414 93
NspI r_CATG'y
Cuts at: 0 625 1605
Size: 625 980
PciI A'CATG_T
Cuts at: 0 621 1605
Size: 621 984
PmlI CAC'GTG
Cuts at: 0 548 1005 1605
Size: 548 457 600
Fragments arranged by size:
600 548 457
PpuMI rG'GwC_Cy
Cuts at: 0 813 1605
Size: 813 792
PshAI GACnn'nnGTC
Cuts at: 0 1353 1605
Size: 1353 252
PvuII CAG'CTG
Cuts at: 0 158 1605
Size: 158 1447
SacI G_AGCT'C
Cuts at: 0 292 354 580 1605
Size: 292 62 226 1025
Fragments arranged by size:
1025 292 226 62
SapI GCTCTTCn'nnn_
Cuts at: 0 568 738 1605
Size: 568 170 867
Fragments arranged by size:
867 568 170
SfcI C'TryA_G
Cuts at: 0 393 1605
Size: 393 1212
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 417 1605
Size: 417 1188
SmlI C'TyrA_G
Cuts at: 0 764 1257 1605
Size: 764 493 348
Fragments arranged by size:
764 493 348
StuI AGG'CCT
Cuts at: 0 469 1605
Size: 469 1136
StyI C'CwwG_G
Cuts at: 0 470 740 1097 1272 1605
Size: 470 270 357 175 333
Fragments arranged by size:
470 357 333 270 175
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 342 1605
Size: 342 1263
TatI w'GTAC_w
Cuts at: 0 78 1605
Size: 78 1527
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 963 995 1605
Size: 963 32 610
Fragments arranged by size:
963 610 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1169 1605
Size: 1169 436
XmnI GAAnn'nnTTC
Cuts at: 0 520 1605
Size: 520 1085
Enzymes that do cut:
AarI AccI AcuI AflIII AhdI AlwNI ApoI AvaI
BanI BanII BbvCI BciVI BglI BglII Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspMI BsrBI BsrDI BssHII BstYI BstZ17I Bsu36I BtgI
BtgZI DraI DrdI EaeI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRV Hin4I HindIII MscI MslI MspA1I
NcoI NheI NspI PciI PmlI PpuMI PshAI PvuII
SacI SapI SfcI SfiI SmlI StuI StyI TaqII
TatI TstI XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AleI AlfI
AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbsI BcgI BclI BfrBI BlpI BplI
BsaBI BseYI BsgI BsiWI BsmBI BspHI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstXI BtsI ClaI CspCI
DraIII EagI EcoRI FalI FseI FspI FspAI HaeII
HincII HpaI KasI KpnI MfeI MluI MmeI NaeI
NarI NdeI NgoMIV NotI NruI NsiI PacI PasI
PflMI PfoI PmeI PpiI PsiI PspOMI PspXI PsrI
PstI PvuI RsrII SacII SalI SanDI SbfI ScaI
SexAI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI SwaI Tth111I XbaI XhoI XmaI ZraI