Restriction map for
pF1KB8840
(Linear) (Six-base) MAPSORT of: KIBB8840.seq Check: 7304 from: 1 to: 1401
KIBB8840.orf
With 182 enzymes: SgfI *
March 3, 2008 11:08 ..
AccI GT'mk_AC
Cuts at: 0 506 1401
Size: 506 895
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 457 1291 1401
Size: 457 834 110
Fragments arranged by size:
834 457 110
AflII C'TTAA_G
Cuts at: 0 553 1401
Size: 553 848
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 281 464 1401
Size: 281 183 937
Fragments arranged by size:
937 281 183
AlwNI CAG_nnn'CTG
Cuts at: 0 162 600 1401
Size: 162 438 801
Fragments arranged by size:
801 438 162
ApaI G_GGCC'C
Cuts at: 0 706 1226 1401
Size: 706 520 175
Fragments arranged by size:
706 520 175
ApaLI G'TGCA_C
Cuts at: 0 37 1401
Size: 37 1364
AvaI C'yCGr_G
Cuts at: 0 461 926 1401
Size: 461 465 475
Fragments arranged by size:
475 465 461
BamHI G'GATC_C
Cuts at: 0 1337 1401
Size: 1337 64
BanI G'GyrC_C
Cuts at: 0 577 758 829 1401
Size: 577 181 71 572
Fragments arranged by size:
577 572 181 71
BanII G_rGCy'C
Cuts at: 0 433 706 820 1226 1332 1401
Size: 433 273 114 406 106 69
Fragments arranged by size:
433 406 273 114 106 69
BbvCI CC'TCA_GC
Cuts at: 0 1178 1401
Size: 1178 223
BglI GCCn_nnn'nGGC
Cuts at: 0 133 895 1063 1401
Size: 133 762 168 338
Fragments arranged by size:
762 338 168 133
BglII A'GATC_T
Cuts at: 0 1191 1401
Size: 1191 210
BlpI GC'TnA_GC
Cuts at: 0 427 1331 1401
Size: 427 904 70
Fragments arranged by size:
904 427 70
Bme1580I G_kGCm'C
Cuts at: 0 41 270 706 761 832 885 1226 1401
Size: 41 229 436 55 71 53 341 175
Fragments arranged by size:
436 341 229 175 71 55 53 41
BmrI ACTGGGnnnn_n'
Cuts at: 0 788 794 1401
Size: 788 6 607
Fragments arranged by size:
788 607 6
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 278 1357 1401
Size: 278 1079 44
Fragments arranged by size:
1079 278 44
Bpu10I CC'TnA_GC
Cuts at: 0 920 959 971 1109 1178 1401
Size: 920 39 12 138 69 223
Fragments arranged by size:
920 223 138 69 39 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 556 1401
Size: 556 845
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 276 306 1401
Size: 276 30 1095
Fragments arranged by size:
1095 276 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 614 1195 1401
Size: 614 581 206
Fragments arranged by size:
614 581 206
BseYI C'CCAG_C
Cuts at: 0 137 1073 1401
Size: 137 936 328
Fragments arranged by size:
936 328 137
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 488 1401
Size: 488 913
BsiHKAI G_wGCw'C
Cuts at: 0 41 306 433 1401
Size: 41 265 127 968
Fragments arranged by size:
968 265 127 41
Bsp1286I G_dGCh'C
Cuts at: 0 41 270 306 433 706 761 820 832
Size: 41 229 36 127 273 55 59 12
Cuts at: 832 885 1226 1332 1401
Size: 53 341 106 69
Fragments arranged by size:
341 273 229 127 106 69 59 55
53 41 36 12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 322 428 1401
Size: 322 106 973
Fragments arranged by size:
973 322 106
BsrDI GCAATG_nn'
Cuts at: 0 1378 1401
Size: 1378 23
BssSI C'ACGA_G
Cuts at: 0 263 878 1401
Size: 263 615 523
Fragments arranged by size:
615 523 263
BstAPI GCAn_nnn'nTGC
Cuts at: 0 162 1401
Size: 162 1239
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1005 1401
Size: 1005 396
BstYI r'GATC_y
Cuts at: 0 117 1102 1191 1337 1401
Size: 117 985 89 146 64
Fragments arranged by size:
985 146 117 89 64
Bsu36I CC'TnA_GG
Cuts at: 0 1235 1401
Size: 1235 166
BtgI C'CryG_G
Cuts at: 0 59 1401
Size: 59 1342
BtsI GCAGTG_nn'
Cuts at: 0 88 204 343 479 598 1401
Size: 88 116 139 136 119 803
Fragments arranged by size:
803 139 136 119 116 88
EaeI y'GGCC_r
Cuts at: 0 374 866 1401
Size: 374 492 535
Fragments arranged by size:
535 492 374
EarI CTCTTCn'nnn_
Cuts at: 0 77 1159 1201 1401
Size: 77 1082 42 200
Fragments arranged by size:
1082 200 77 42
EcoICRI GAG'CTC
Cuts at: 0 431 1401
Size: 431 970
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 278 457 1291 1357 1401
Size: 278 179 834 66 44
Fragments arranged by size:
834 278 179 66 44
EcoNI CCTnn'n_nnAGG
Cuts at: 0 82 1401
Size: 82 1319
EcoO109I rG'GnC_Cy
Cuts at: 0 75 159 540 702 703 727 788 1223
Size: 75 84 381 162 1 24 61 435
Cuts at: 1223 1249 1401
Size: 26 152
Fragments arranged by size:
435 381 162 152 84 75 61 26
24 1
FspI TGC'GCA
Cuts at: 0 196 1401
Size: 196 1205
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 102 134 1285 1317 1401
Size: 102 32 1151 32 84
Fragments arranged by size:
1151 102 84 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 204 1401
Size: 204 1197
MscI TGG'CCA
Cuts at: 0 376 868 1401
Size: 376 492 533
Fragments arranged by size:
533 492 376
MspA1I CmG'CkG
Cuts at: 0 1326 1335 1401
Size: 1326 9 66
Fragments arranged by size:
1326 66 9
NspI r_CATG'y
Cuts at: 0 22 409 1401
Size: 22 387 992
Fragments arranged by size:
992 387 22
PasI CC'CwG_GG
Cuts at: 0 730 825 1079 1401
Size: 730 95 254 322
Fragments arranged by size:
730 322 254 95
PflMI CCAn_nnn'nTGG
Cuts at: 0 144 1401
Size: 144 1257
PpuMI rG'GwC_Cy
Cuts at: 0 788 1401
Size: 788 613
PspOMI G'GGCC_C
Cuts at: 0 702 1222 1401
Size: 702 520 179
Fragments arranged by size:
702 520 179
PstI C_TGCA'G
Cuts at: 0 158 333 605 1401
Size: 158 175 272 796
Fragments arranged by size:
796 272 175 158
SacI G_AGCT'C
Cuts at: 0 433 1401
Size: 433 968
SanDI GG'GwC_CC
Cuts at: 0 788 1401
Size: 788 613
SbfI CC_TGCA'GG
Cuts at: 0 158 333 1401
Size: 158 175 1068
Fragments arranged by size:
1068 175 158
SfcI C'TryA_G
Cuts at: 0 154 216 329 601 1401
Size: 154 62 113 272 800
Fragments arranged by size:
800 272 154 113 62
SmaI CCC'GGG
Cuts at: 0 463 928 1401
Size: 463 465 473
Fragments arranged by size:
473 465 463
SmlI C'TyrA_G
Cuts at: 0 535 553 1401
Size: 535 18 848
Fragments arranged by size:
848 535 18
SphI G_CATG'C
Cuts at: 0 409 1401
Size: 409 992
SrfI GCCC'GGGC
Cuts at: 0 928 1401
Size: 928 473
StyI C'CwwG_G
Cuts at: 0 848 890 911 932 938 992 1019 1025
Size: 848 42 21 21 6 54 27 6
Cuts at: 1025 1037 1052 1058 1401
Size: 12 15 6 343
Fragments arranged by size:
848 343 54 42 27 21 21 15
12 6 6 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 502 565 1401
Size: 502 63 836
Fragments arranged by size:
836 502 63
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 219 251 276 308 333 365 1401
Size: 219 32 25 32 25 32 1036
Fragments arranged by size:
1036 219 32 32 32 25 25
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 570 945 1059 1401
Size: 570 375 114 342
Fragments arranged by size:
570 375 342 114
XmaI C'CCGG_G
Cuts at: 0 461 926 1401
Size: 461 465 475
Fragments arranged by size:
475 465 461
Enzymes that do cut:
AccI AcuI AflII AlfI AlwNI ApaI ApaLI AvaI
BamHI BanI BanII BbvCI BglI BglII BlpI Bme1580I
BmrI BpmI Bpu10I BpuEI BsaXI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspMI BsrDI BssSI BstAPI BstXI BstYI
Bsu36I BtgI BtsI EaeI EarI EcoICRI Eco57MI EcoNI
EcoO109I FspI Hin4I MmeI MscI MspA1I NspI PasI
PflMI PpuMI PspOMI PstI SacI SanDI SbfI SfcI
SmaI SmlI SphI SrfI StyI TaqII TstI XcmI
XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflIII AgeI AhdI
AleI AloI ApoI AscI AseI AsiSI AvrII BaeI
BbeI BbsI BcgI BciVI BclI BfrBI BmgBI BmtI
BplI BsaI BsaAI BsaBI BsaHI BsaWI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BsrBI BsrFI BsrGI BssHII
BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI DraIII
DrdI EagI EciI EcoRI EcoRV FalI FseI FspAI
HaeII HincII HindIII HpaI KasI KpnI MfeI MluI
MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PciI PfoI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PvuI PvuII RsrII SacII
SalI SapI ScaI SexAI SfiI SfoI SgfI SgrAI
SnaBI SpeI SspI StuI SwaI TatI Tth111I XbaI
XhoI XmnI ZraI