Restriction map for
pF1KB8839
(Linear) (Six-base) MAPSORT of: KIBB8839.seq Check: 9204 from: 1 to: 1398
KIBB8839.orf
With 182 enzymes: SgfI *
March 3, 2008 11:08 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1031 1398
Size: 1031 367
ApaI G_GGCC'C
Cuts at: 0 1203 1398
Size: 1203 195
ApaLI G'TGCA_C
Cuts at: 0 1238 1398
Size: 1238 160
AvaI C'yCGr_G
Cuts at: 0 629 1139 1398
Size: 629 510 259
Fragments arranged by size:
629 510 259
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 354 387 993 1026 1398
Size: 354 33 606 33 372
Fragments arranged by size:
606 372 354 33 33
BamHI G'GATC_C
Cuts at: 0 91 801 1398
Size: 91 710 597
Fragments arranged by size:
710 597 91
BanI G'GyrC_C
Cuts at: 0 982 1398
Size: 982 416
BanII G_rGCy'C
Cuts at: 0 468 847 1203 1398
Size: 468 379 356 195
Fragments arranged by size:
468 379 356 195
BbsI GAAGACnn'nnnn_
Cuts at: 0 1037 1351 1398
Size: 1037 314 47
Fragments arranged by size:
1037 314 47
BclI T'GATC_A
Cuts at: 0 596 1398
Size: 596 802
BfrBI ATG'CAT
Cuts at: 0 971 1398
Size: 971 427
Bme1580I G_kGCm'C
Cuts at: 0 1203 1242 1398
Size: 1203 39 156
Fragments arranged by size:
1203 156 39
BmrI ACTGGGnnnn_n'
Cuts at: 0 388 1398
Size: 388 1010
BmtI G_CTAG'C
Cuts at: 0 301 1398
Size: 301 1097
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 145 452 784 1398
Size: 145 307 332 614
Fragments arranged by size:
614 332 307 145
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 9 847 1121 1398
Size: 9 838 274 277
Fragments arranged by size:
838 277 274 9
BseYI C'CCAG_C
Cuts at: 0 745 1398
Size: 745 653
BsiHKAI G_wGCw'C
Cuts at: 0 770 847 1223 1242 1398
Size: 770 77 376 19 156
Fragments arranged by size:
770 376 156 77 19
Bsp1286I G_dGCh'C
Cuts at: 0 468 770 847 1203 1223 1242 1398
Size: 468 302 77 356 20 19 156
Fragments arranged by size:
468 356 302 156 77 20 19
BspHI T'CATG_A
Cuts at: 0 602 1398
Size: 602 796
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 409 1398
Size: 409 989
BsrDI GCAATG_nn'
Cuts at: 0 327 1335 1398
Size: 327 1008 63
Fragments arranged by size:
1008 327 63
BsrGI T'GTAC_A
Cuts at: 0 236 1398
Size: 236 1162
BstEII G'GTnAC_C
Cuts at: 0 105 1398
Size: 105 1293
BstXI CCAn_nnnn'nTGG
Cuts at: 0 414 933 1398
Size: 414 519 465
Fragments arranged by size:
519 465 414
BstYI r'GATC_y
Cuts at: 0 91 801 1398
Size: 91 710 597
Fragments arranged by size:
710 597 91
BtgI C'CryG_G
Cuts at: 0 1028 1398
Size: 1028 370
BtsI GCAGTG_nn'
Cuts at: 0 756 1398
Size: 756 642
DraI TTT'AAA
Cuts at: 0 1353 1398
Size: 1353 45
DraIII CAC_nnn'GTG
Cuts at: 0 1262 1398
Size: 1262 136
DrdI GACnn_nn'nnGTC
Cuts at: 0 313 1398
Size: 313 1085
EarI CTCTTCn'nnn_
Cuts at: 0 484 1398
Size: 484 914
EcoICRI GAG'CTC
Cuts at: 0 845 1398
Size: 845 553
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 145 452 784 1031 1398
Size: 145 307 332 247 367
Fragments arranged by size:
367 332 307 247 145
EcoO109I rG'GnC_Cy
Cuts at: 0 274 444 1200 1398
Size: 274 170 756 198
Fragments arranged by size:
756 274 198 170
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1365 1398
Size: 1365 33
HaeII r_GCGC'y
Cuts at: 0 1391 1398
Size: 1391 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 844 876 940 972 1398
Size: 844 32 64 32 426
Fragments arranged by size:
844 426 64 32 32
HincII GTy'rAC
Cuts at: 0 150 171 1398
Size: 150 21 1227
Fragments arranged by size:
1227 150 21
MfeI C'AATT_G
Cuts at: 0 1268 1398
Size: 1268 130
MslI CAynn'nnrTG
Cuts at: 0 412 607 1398
Size: 412 195 791
Fragments arranged by size:
791 412 195
MspA1I CmG'CkG
Cuts at: 0 436 1398
Size: 436 962
NheI G'CTAG_C
Cuts at: 0 297 1398
Size: 297 1101
NsiI A_TGCA'T
Cuts at: 0 973 1398
Size: 973 425
NspI r_CATG'y
Cuts at: 0 744 971 1398
Size: 744 227 427
Fragments arranged by size:
744 427 227
PasI CC'CwG_GG
Cuts at: 0 797 933 1398
Size: 797 136 465
Fragments arranged by size:
797 465 136
PpuMI rG'GwC_Cy
Cuts at: 0 274 444 1398
Size: 274 170 954
Fragments arranged by size:
954 274 170
PsiI TTA'TAA
Cuts at: 0 53 1376 1398
Size: 53 1323 22
Fragments arranged by size:
1323 53 22
PspOMI G'GGCC_C
Cuts at: 0 1199 1398
Size: 1199 199
PvuII CAG'CTG
Cuts at: 0 436 1398
Size: 436 962
SacI G_AGCT'C
Cuts at: 0 847 1398
Size: 847 551
SmaI CCC'GGG
Cuts at: 0 1141 1398
Size: 1141 257
SspI AAT'ATT
Cuts at: 0 1077 1398
Size: 1077 321
TatI w'GTAC_w
Cuts at: 0 236 1398
Size: 236 1162
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 266 298 508 540 704 736 1232 1264
Size: 266 32 210 32 164 32 496 32
Cuts at: 1264 1398
Size: 134
Fragments arranged by size:
496 266 210 164 134 32 32 32
32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1164 1398
Size: 1164 234
XmaI C'CCGG_G
Cuts at: 0 1139 1398
Size: 1139 259
Enzymes that do cut:
AcuI ApaI ApaLI AvaI BaeI BamHI BanI BanII
BbsI BclI BfrBI Bme1580I BmrI BmtI BpmI BseRI
BseYI BsiHKAI Bsp1286I BspHI BspMI BsrDI BsrGI BstEII
BstXI BstYI BtgI BtsI DraI DraIII DrdI EarI
EcoICRI Eco57MI EcoO109I FalI HaeII Hin4I HincII MfeI
MslI MspA1I NheI NsiI NspI PasI PpuMI PsiI
PspOMI PvuII SacI SmaI SspI TatI TstI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApoI AscI
AseI AsiSI AvrII BbeI BbvCI BcgI BciVI BglI
BglII BlpI BmgBI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BsgI BsiEI BsiWI BsmI
BsmBI BspEI BsrBI BsrFI BssHII BssSI BstAPI BstBI
BstZ17I Bsu36I BtgZI ClaI CspCI EaeI EagI EciI
EcoNI EcoRI EcoRV FseI FspI FspAI HindIII HpaI
KasI KpnI MluI MmeI MscI NaeI NarI NcoI
NdeI NgoMIV NotI NruI PacI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PspXI PsrI PstI PvuI
RsrII SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI
SphI SrfI StuI StyI SwaI TaqII Tth111I XbaI
XhoI XmnI ZraI